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This is the Progress in Histochemistry and Cytochemistry publishes comprehensive and analytical reviews within the entire field of histochemistry and cytochemistry.

这是《组织化学和细胞化学的进展》的网页。

Objective To study the significance of expression of cyclooxygenase-2(COX-2)and vascular endothelial growth factorin breast cancerand its correlation.Methods Paraffin imbedding tissues from 60 cases with BC were detected for expression of COX-2 and VEGF by immunohistochemistry,the relationship of its expression with clinical pathological parameters were analyzed.

目的 探讨环氧化酶-2(COX-2)和血管内皮细胞生长因子在乳腺癌组织中的表达及其表达之间的关系方法 S-P免疫组织化学方法检测60例乳腺癌石蜡包埋组织中COX-2和VEGF的表达,分析其表达之间的关系及其与临床病理参的关系。

In this study, the expression and function of FGF-13 in developmental follicles and skin during embryonic development,normal vibrissa follilce stem cells and wound follicles in vitro were obserbed,so as to illuminate the possible role of FGF-13 in the skin follicles during embryonic development and damage healing and provide the experimental base for further studying the characteristic and mechanism of the role of FGF-13 in skin.

采用免疫组织化学(Immunohistochemistry,IHC)、免疫细胞化学(Immunocytochemistry, ICC)和原位杂交(in situ hybridization,ISH)技术及体外大鼠触须毛囊的器官培养,研究了三种不同的生物学过程中FGF-13在皮肤毛囊中的表达及作用。

To obtain precise result, itis necessary, in the aspect of hardware, to set up one stable optics system with highSNR, only if so, can we do the measurement with the steady NIR. To do so, we designthe optics system using AOTF, and take some measures to increase the light energyand the system SNR, which includes the three layers structure of the light chamber,the equipments to cover the useless light, polarizing to reduce the influence of 0 gradelight and focalize the light with lens. Apart from the hardware aspect, according to theexperiments, we can see that the different temperatures between at setting up themodels and at predicting the results will affect the prediction results in a way, and theaffection extent will be increased along with the increase of the temperaturedifference. Moreover, the light energy that can be detected will be different with thecontact pressure, and the match of the refractive indexes between the tissue and thesurroundings will enhance the light transmission depth, which will take moreinformation of the tissue. So, during the measurement, we should ensure the sametemperature between measuring and predicting, the same contact pressure at everymeasurement and the match of the indexes between the tissue and the surroundings aspossible as we can.

要提高测量精度,从硬件方面就必须有一个稳定的、信噪比较高的分光光路系统,以此得到稳定的近红外光来进行无创检测,针对这个目的,我们设计了 AOTF 分光光路系统,并在系统中采用一定的措施如三层灯室结构、遮光筒以减小杂散光、起偏和检偏来减小 0 级光影响、透镜汇聚等等来提高出射光的能量和系统的信噪比;从其他影响因素方面,通过实验,我们得到不同的建模温度对于预测结果有一定的影响,且影响随着温度的差值越大而越大,另外散反射光谱的能量也随着接触压力的不同而不同,组织的折射率与环境匹配将加大光的入射深度,从而能携带更多的组织信息,所以在测量时我们尽可能保证同一温度、保证同一接触压力以及利用物理或者化学的方法使组织与环境折射率匹配等。

According to previous studies, the hosts —parasites immune interactions were investigated in rabbits following immunization with extracts from Ixodes sinensis basing on medical entomology, immunology, biochemistry, pathology and histochemistry and reported from the following eight fields

本研究在以往研究工作的基础上,首次选用我国江南地区具有重要医学和兽医学意义的优势蜱种:中华硬蜱的不同抗原对新西兰兔进行免疫接种,对宿主—寄生虫免疫相互作用从医学昆虫学、免疫学、生物化学、病理学、组织化学等不同方面进行研究,就以下8个方面首次进行报道。

Methods Healthy Sprague-Dawley rats received basal ovalbumin-sensitized combined with intra-esophageal mucosal acid exposure. The neuronal expressions of c-fos proto-oncogene were detected with immunohistochemical counter-staining and computerized color image analyzer.

采用腹腔注射鸡卵清蛋白基础致敏联合食管酸灌注的方法处理SD大鼠,并采用免疫组织化学方法和显微图像分析技术评价内脏高敏感性-食管化学刺激大鼠模型的可靠性。

GFAP overexpessed but NOS neuron decreased, distorted and pale. The glia in SM-DB complex of ONR hypertrophied, but NOS neuron no degeneration. The increased GFAP immunoreactivity and the morphometric and cytological changes in SM-DB complex astrocytes may reflect a sustained upregulation of cellular activity with the insults of neurons, resulting in hypertrophy of glial perikarya and cell processes.

老年减退组星形胶质细胞的细胞体密度较老年正常组和青年组明显增加(P 在GFAP免疫细胞化学和NOS组织化学双重染色的切片上,老年减退大鼠SM-DB星形胶质细胞明显肥大,突起粗壮弯曲,GFAP超量表达,而NOS阳性神经元数量减少,胞体变形,突起减少,染色深度下降。

The cytochemistrialstaining was used to detect the activities of PAMs.The results showed that the activities of ACPaseat the early stage of postinoculation,the activities of glycogen or glycoprotein,ATPase and ANAEin PRRSV-infected PAMs were markedly decreased.

用酶组织化学染色法对感染PRRSV的仔猪肺泡巨噬细胞内的细胞化学成分进行了动态研究,发现PRRSV急性感染早期肺泡巨噬细胞ACPase活性减弱,感染后各时期肺泡巨噬细胞内糖类、ATPase和ANAE活性均减弱。

Methods: expression of cox-2 gene in 60 cases of breast carcinoma tissues was detected by sp immunohistochemistry.

采用免疫组织化学 sp法,检测60例乳腺癌组织中环氧化酶-2的表达情况。

Methods : Using immunohistochemical method, expression of VEGF and MVD in 50 operation cases of carcinoma of endometrium and 30 normal cases were examined.

采用免疫组织化学方法检测 50例子宫内膜癌和 30例正常子宫内膜组织中 VEGF和 MVD的表达。

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