组织化学的

MATERIALS AND METHODS: The protein expressions of MMP-7 and MMP-9 were evaluated by immunohistochemistry in 18 normal villi. 49 hydatidiform moles, in which 8 were malignantly transformed moles.

材料与方法：采用免疫组织化学PV-6000法测定49例葡萄胎（其中8例恶变，列为葡萄胎恶变组）和18例正常绒毛组织中的MMP-7和MMP-9蛋白表达量。

The result of experiments revealed that the separation of resistant gene in the transgenic plant progeny was consistent with Mendel's law of segregation.

组织化学定位证实GUS主要集中在维管束组织，说明这一反式调控系统在转基因植株中能有效地调节gusA基因的表达。

Methods The gingival cancer tissue of 24 cases and normal gingival tissue of 7 cases were studied with histochemical and electron microscopical methods.

方法对24例牙龈癌和正常组织中的肥大细胞用组织化学方法结合电子显微镜进行观察。

The CNTF and FGF2 positive cell number and the positive area percentage, as well as the number of neuron and neuroglia calculated by immunofluorescence analysis in the spinal cord.

将嗅鞘细胞移植入脊髓半横断SD大鼠，观察术后7天和21天嗅鞘细胞在脊髓内的存活情况，并对脊髓组织切片进行CNTF和FGF2免疫荧光组织化学染色，计数阳性细胞并计算阳性面积百分比。

Methods The expression of CD147 and MMP-2 proteins were detected by immunohistochemical method in 46 cases of gliomas, and compared with those in 14 cases of normal brain tissues. The correlation between them was analyzed.

应用免疫组织化学链霉素抗生物素蛋白－过氧化酶连接法，检测46例胶质瘤瘤组织中CD147蛋白及MMP-2蛋白的表达，分别与正常脑组织14例进行对照。

Methods The expression of survivin was detected by S-P immunohistochemical technique in 46 cases of human osteosarcoma.

应用免疫组织化学S-P法对46例原发性骨肉瘤组织中survivin蛋白的表达水平进行检测。

The samples were fixed with paraformaldehyde and embedded in paraffin.

应用免疫组织化学技术检测鼻粘膜组织中水通道蛋白1、2、3、4、5的分布情况。

METHODS: A total of 129 gastric mucosa biopsies and its corresponding serum specimens were collected from patients with superficial gastritis (n=30), gastric ulcer or erosion (n=35), atrophic gastritis (29), and gastric cancer (n=35). The expression of PGC in the gastric mucosa was detected by immunohistochemistry, and the concentration of serum pepsinogen A and pepsinogen C were determined by enzyme linked immunosorbent assay.

129例胃黏膜活检组织及其血清标本（浅表性胃炎30例，胃黏膜糜烂溃疡35例，萎缩性胃炎29例，胃癌35例），采用免疫组织化学染色检测胃黏膜标本中PGC的表达情况；采用酶联免疫吸附实验检测血清PGA和PGC含量。

Methods:The expression of CK20mRNA in portal venous blood from 30 colorectal cancer patients was detected by fluorescent quarto RTPCR,and the results of CD44v6 and PCNA in colorectal cancer tissue were determined by means of immunohistochemistry,and then compared with control groups through statistics analysis.

应用荧光定量RTPCR法检测50例大肠癌患者回流门静脉血中CK20mRNA，同时应用免疫组织化学方法测定大肠癌癌组织中CD44v6、PCNA的表达，并与对照组比较。

Methods The expression of COX-2 and Survivin of 30 cases of SACC and 20 cases of normal salivary gland was examined with immunohistochemical technique.

采用免疫组织化学检测30例涎腺腺样囊性癌及20例正常涎腺组织中COX-2与Survivin蛋白的表达情况。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。