组织化学的

Animals after chemolysis failure to homogenate respectively,centrifugate and take the pure liquid,mcasure collagenase activity with Blanckaert method,the data handled with statistics did not show obvious difference.

组织化学检测：将实验动物组、对照组、手术组和化学溶解术失败再手术组标本的纤维环内层、中层和外层分别匀浆、离心，取上清液，按Blanckaert法测定胶原酶，所得据经统计学处理差异无显著性。

In ACC, NGF,TrkA and p75 mainly existed in cells of duct-like, cribriform and mass-like structures.According to the optical density scanning of immunohistochemistric stain of NGF and TrkA in salivary gland and tumor tissues and quantification analysis, it was shown that the average optical density of NGF and TrkA increased in order of salivary gland tissues around neoplasm, mucoepidermiod carcinoma, and adenoid cystic carcinoma.

2叫 吉林大学硕士学位论文通过对NGF和TrkA在涎腺组织和涎腺肿瘤中的免疫组织化学染色结果进行光密度扫描，对所测平均光密度定量分析，结果显示：NGF和TrkA平均光密度值按照肿瘤周围涎腺组织、豁液表皮样癌和腺样囊性癌依次升高，统计学分析在各组间均有极显著差异(P.01)，表明NGF和TrkA通过自分泌机制调控涎腺腺样囊性癌和豁液表皮样癌的生长与增殖，通过旁分泌机制参与涎腺腺样囊性癌向神经周围的浸润。

Objective To study the relationship between IL-1,MMP-13and pathogenesis of knee osˉteoarthrosis.Methods IL-1and MMP-13were measured concurrently in synovial fluid samples from23patients with mild OA,28patients with severe OA and6patients with disciform meniscus.The determinations of positive rate of cells in genual synovium with IL-1and MMP-13were carried out using immunohistochemical techonology.

目的 探讨白细胞介素-1（IL-1）、基质金属蛋白酶-13（MMP-13）在膝关节骨性关节炎发病中的作用方法提取23例OA病变轻微患者、28例OA病变严重患者、6例对照者的滑膜组织及关节液，采用免疫组织化学方法检测膝关节滑膜组织中IL-1、MMP-13的阳性细胞率及通过夹心ELISA法测定膝关节滑液中IL-1、MMP-13含量。

Sixty-three cases of AFP-positive gastric carcinoma were divided into four subtype: hepatoid type 22 cases (34.9％), fetal gastrointestinal type 15 cases (23.8%), and mixed type of the hepatoid type with enteroblastic differentiation to be 24 cases(38.1%), and yolk sac tumor-like type 2 cases (3.2％).

应用光镜、电镜、组化和免疫组织化学方法，对87例AFP阳性胃癌中临床病理资料较完整的63例进行病理组织形态观察，并用2例3个月妊娠的胚胎胃肠组织以同样方法行相关的对比研究。

Methods: The experimental type Ⅱ diabetic rats were induced by injection of strepiozotocin (STZ, 30mg/kg ) and fed with high fat and high glucose fond. The rats were divided into three groups: a normal conrmal control group, a diabetes group and a treated group. The treated group were hypodermically injected insulin 3U/d at interfemus for 4 months, white DC and NC group were vehicle groups that were treated with the same close saline, By using SABC method, thee express ion of collagen type Ⅰ and Ⅲ in SAN was detected.

高糖高脂饮食加小剂量链脲佐菌素腹腔注射(STZ，30mg/kg)制备Ⅱ型糖尿病大鼠模型，并随机分为正常对照组、糖尿病组和胰岛素治疗组（每只3U/d股内侧皮下注射），对照组和糖尿病组注射等剂量的生理盐水每天1次，持续4个月；窦房结组织行SABC法免疫组织化学显色，检测窦房结组织中的Ⅰ型和Ⅲ胶原的表达及变化。

Methods: HPV 16/18 infection was detected by nested-polymerase chain reaction, the p53 mutation was detected by direct sequencing, and the p53 and the HPV 16/18 E6 proteins were studied using immunohistochemistry on 129 pterygial specimens and 20 normal conjunctivas.

本研究利用巢叠式聚合脢锁反应分析自129例罹患翳状赘肉之翳状赘肉组织及20例正常无翳状赘肉的结膜组织检体中，是否有人类乳突病毒16及18型的感染，并利用直接定序的方式检测p53基因是否发生突变，此外亦以免疫组织化学染色法侦测p53蛋白与人类乳突病毒E6蛋白。

ABSTRACT Objective To discuss the clinical pathologic features and differential diagnosis of mixed germ cell sex cord stromal tumorwith malignant variant of germ cell tumor Methods The clinical pathologic datum and immunophenotype were studied in the case of ovary MGCSCST with malignant variance of germ cell tumor Results The patient's genital anatomy and female phenotype were normal,karotypes was 46xx with uterogestation The tumor tissue consisted of mixed germ cells and sex cord stromal cells with evident malignancy of mixed germ cells There was no atypical structure of gonadoblastoma in tumor tissue Conclusion MGCSCSST has complex morphosis,thus,to prevent misdiagnosis,more samples should be collected and more slices be cut The patient may have prognosis malo when there are high malignant variants of mixed germ cells in tumor tissue

目的 探讨混合性生殖细胞性索间质肿瘤(mixed germ cell sex cord stromal tumor，MGCSCST)伴恶性生殖细胞肿瘤变异型的临床病理特点及鉴别诊断。方法卵巢MGCSCST伴恶性生殖细胞肿瘤变异型的病例进行临床病理特征和免疫表型分析。结果患者生殖器的解剖结构和女性表型正常，染色体组型是46xx，足月妊娠。肿瘤组织由混合性生殖细胞和性索间质肿瘤构成，混合性生殖细胞有显著的恶性特征。瘤组织中没有典型的性腺母细胞瘤结构。结论 MGCSCST有复杂的形态结构，因此更多的取材和切片可以防止误诊，当肿瘤组织中的混合性生殖细胞有高度恶性变异时，患者预后不良。卵巢肿瘤；生殖细胞性索间质肿瘤；免疫组织化学

Methods Samples from 78 patients with pituitary adenomas, including 37 invasive and 41 noninvasive, and 5 normal pituitary tissue specimens from autopsied patients were examined. The expressions of OPN mRNA and OPN were examined by real-time fluorescence quantitative PCR and immunohistochemical SP method, respectively.

分别对78例垂体腺瘤手术标本（其中侵袭性37例，非侵袭性41例）和5例来源于尸检的正常垂体组织进行检测，采用实时荧光PCR技术检测组织中OPN mRNA表达，免疫组织化学SP法检测OPN蛋白表达，结合临床资料分析OPN mRNA及其蛋白的表达与垂体腺瘤侵袭性的关系。

Methods samples from 78 patients with pituitary adenomas, including 37 invasive and 41 noninvasive, and 5 normal pituitary tissue specimens from autopsied patients were examined. the expressions of opn mrna and opn were examined by real-time fluorescence quantitative pcr and immunohistochemical sp method, respectively. the relation of opn and opn mrna expressions to invasiveness of pituitary adenomas was analyzed.

分别对78例垂体腺瘤手术标本（其中侵袭性37例，非侵袭性41例）和5例来源于尸检的正常垂体组织进行检测，采用实时荧光pcr技术检测组织中opn mrna表达，免疫组织化学sp法检测opn蛋白表达，结合临床资料分析opn mrna及其蛋白的表达与垂体腺瘤侵袭性的关系。

Objective To investigate the distribution of basophils in airway walls of fatal asthmatics, nonfatal asthmatics and non-asthmatics by using a novel monoclonal antibody.

目的 采用一种新的针对嗜碱性粒细胞的单克隆抗体，对不同程度支气管哮喘患者支气管肺组织进行免疫组织化学检测，以明确嗜碱性粒细胞在不同组织结构中的分布及其临床意义。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。