组织化学的

Animals after chemolysis failure to homogenate respectively,centrifugate and take the pure liquid,mcasure collagenase activity with Blanckaert method,the data handled with statistics did not show obvious difference.

组织化学检测：将实验动物组、对照组、手术组和化学溶解术失败再手术组标本的纤维环内层、中层和外层分别匀浆、离心，取上清液，按Blanckaert法测定胶原酶，所得数据经统计学处理差异无显著性。

Methodological contributions as well as papers in the fields of applied histo- and cytochemistry e.g.

该杂志发表整个组织化学和细胞化学领域内的综合和分析评论文章。

The protein and phospholipid constituent in membrane of neurocyte in brain of chicken with encephalomalacia was separated and studied with the method of SDS-PAGE electrophoresis and the method of high performance-thin layer chromatography respectively The fluidity of neurocyte membrane of chicken with encephalomalacia was determined for the first time The content of Ca and trace elements were determined in chicken encephalomalacia systematically and the status of Fe ion in cerebellum was observed. The activity of enzymes that were relative to metabolism of energy and neuro-medium in brain were checked systematically with the method of enzyme-histochemistry. Pathological examination was carried out by microscopic and ultramicroscopic observation. The apoptosis of neurocyte in brain was checked.

在进行临床观察、定期剖杀并检测体内V〓含量、体重和脑重的基础上，重点研究了与神经损伤有关的以下几个方面的内容：系统地检测了体内自由基代谢和抗氧化系统功能的变化；首次采用SDS-PAGE电泳法分离并检测了雏鸡脑软化症神经细胞膜蛋白组分，并以高效薄层层析法检测神经细胞膜磷脂组分；测定了脑软化症雏鸡神经细胞膜的膜流动性；首次系统检测了脑软化症雏鸡体内钙及微量元素含量，并进行了小脑组织总铁离子状态观察；采用酶组织化学方法系统地观察了脑组织中与脑能量代谢和神经递质有关的酶的活性；对相关组织进行了病理组织学和超微结构观察；并对脑组织中神经细胞的凋亡进行了检测。

Tissue samples were examined on 0, 7, 14, 21, 28 and 35 d with micro section paraffin and histochemistry staining method.

分别于0，7，14，21，28和35d取材，利用显微石蜡切片、组织化学染色的方法检测其肝、消化道和肌肉细胞糖原、酸性磷酸酶和碱性磷酸酶细胞化学特征。

Tissue samples were examined on 0, 7, 14, 21, 28 and 35day with micro section paraffin and histochemistry staining method.

分别于0、7、14、21、28和35 d取材，利用显微石蜡切片、组织化学染色的方法检测其肝、肠和肌肉细胞糖原、酸性磷酸酶和碱性磷酸酶细胞化学特征。

The expression pattern of PKC and morphology were investigated during inflammatory pain using immunocytochemistry and histochemistry.

应用组织化学和免疫细胞化学方法观察炎性痛过程中海马神经元形态学变化和PKC活性的改变。

Methods Mouse models of tibia fracture healing were established, and callus samples were collected 1, 3, 7, 14, 21 and 28 days after fracture. The development of callus and new bone formation were evaluated with roentgenology, Micro-CT and tetracycline double labeling method, and the expression of HIF-1α, vascular endothelial growth factor, Runx2 and ALP in callus were detected with RT-PCR, Western blotting and immunohistochemistry. The relationship between HIF-1α and fracture healing was analysed.

建立小鼠胫骨骨折模型，骨折后1、3、7、14、21、28 d取材，应用X线摄片、Micro-CT和四环素荧光双标记技术，观测骨痂组织的演变和新骨形成状况；采用RT-PCR、Western blotting和免疫组织化学方法，检测骨痂组织细胞HIF-1α、血管内皮生长因子和成骨性标志物（Runx2和ALP）的表达状况，分析HIF-1α与骨折愈合进程的关系。

Methods: The immunohistochemistry was used to investigate the protein expression of p27 in 46 cases of osteosarcoma and 16 cases of osteochondroma. The expression of p27 and histological category and differentiation levels of osteosarcoma were observed.

应用S-P免疫组织化学方法检测46例骨肉瘤和16例骨软骨瘤组织中p27的表达，并观察p27表达在骨肉瘤组织学分型、分化程度等临床指标之间的差异。

MethodsPrimary nasal pterygial tissues(15 eyes) and normal human conjunctival tissues(8 eyes) were determined by immunohistochemical staining, using monoclonal and polyconal antibodies specifically against MMP-3, LN and col Ⅲ. The intensity of reaction on individual sections was semiquantitatively graded.

方法应用S-P免疫组织化学染色方法检测15例翼状胬肉组织及8例正常球结膜组织中MMP-3、LN、col Ⅲ的免疫学定位及表达情况，用Masson及VG染色法检测各标本中胶原纤维及弹力纤维的分布情况。

Methods The expression of TP and MVD was detected by immunohistochemistry method.

采用免疫组织化学方法检测 2 5 1例癌组织和相对应的 92例正常组织TP和微血管密度的表达。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失