组织化学的

Methods By immunohistochemical method, the expression of bcl-2 and bad gene were observed in 8 cases of normal breast tissues 19 cases of breast hyerplasia, 20 cases of breast atypical, 48 cases of breast cancer in situs ,and test the expression of ER , PR and bcl-2 , bad in the breast cancer, Eight normal breast tissues were used control.

利用免疫组织化学染色SABC法，原位观察19例乳腺单纯性增生，20例乳腺非典型增生，48例乳腺癌组织中bcl-2、bad蛋白的表达以8例正常乳腺组织作对照。

Immunohistochemical stains were used to show the tryptase + mast cell and the chymase + mast cell distribution in order to observe the change of the mast cell in the model group, the control group and the treatment group.

取右下肺组织，用抗类胰蛋白酶单克隆抗体和抗类糜蛋白酶单克隆抗体免疫组织化学染色观察实验组与正常组之间肥大细胞数量的变化，HE染色观察肺组织一般病理变化。

It will provide the rational for exploring the ideal treatment for liver cancer by detecting the expression of c-Met,hTERT and MAGE-1,which has high expression in liver cancer,in cancer tissues and tissues surrounding tumor by immunohistochemistry,analyzing the different expression of gene in tumor tissue and tissues with different distance from tumor,and then initially debate the molecular boundary in liver tumor exairesis.

通过免疫组织化学方法检测原发性肝细胞癌组织中高表达基因c-Met、hTERT、MAGE-1的表达以及它们在肝癌癌周组织中的表达，分析基因在肝癌组织和癌周不同距离组织的差异表达，初步探讨肝癌的分子切缘，为探索新的治疗肝癌途径奠定坚实的理论基础。

Methods The expressions of Rb protein were detected in 52 patients with breast cancer, 20 patients with breast fibroadenoma or breast cystic hyperplasia by S-P immunohistochemical method.

采用S-P免疫组织化学染色法对52例乳腺癌组织和20例良性乳腺病变组织中Rb蛋白的表达进行检测。

Results The results showed that the number of mast cells around the periphery of gingival cancer tissue increased significantly.

结果 在牙龈癌周围组织中肥大细胞显著增多，在组织化学上与正常牙龈组织的肥大细胞不同，不含肝素。

Methods The expressions of PCNA were checked by means of immunohistochemistry in the tissue of hydatidiform mole of 32 cases.

葡萄胎组织32例，应用免疫组织化学方法染色检测葡萄胎组织的PCNA表达。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Methods: Immunohistochemistry was applied to examine the expression of PTEN, P27 and cyclin D1 in 58 cases of human colorectal carcinoma, 58 cases of tumor sideward mucosa and 14 cases of normal colorectal mucosa.

应用免疫组织化学方法检测58例大肠癌组织、58例癌旁组织和14例正常大肠黏膜中PTEN、 p27和cyclin D1蛋白的表达情况。

Positive expressive rate of cyclin D1 in colorectal carcinoma was 60.34%（35/58）, which was higher than that in tumor sideward mucosa and normal colorectal mucosa.

应用免疫组织化学方法检测58例大肠癌组织、58例癌旁组织和14例正常大肠黏膜中PTEN、 p27和cyclin D1蛋白的表达情况。

Methods Sixty adult healthy male Wistar rats were enrolled in the experiment. PTSD-like rat model was established by single prolonged stress. There was no SPS stimulation in the control group and the rats of the other five groups were undisturbedly raised for 24 hours, 4 days,7 days,14 days and 28 days respectively after SPS stimulation.The expressions of glucocorticoid receptors in the locus ceruleus nucleus of all groups were detected with Nissl staining, immunohistochemistry,Western blotting and PCR, and image analysis and statistical analysis were performed.

成年健康雄性Wistar大鼠60只，使用无连续单一应激方法建立PTSD大鼠模型，分别取SPS处理后24h、4d、7d、14d和28d大鼠蓝斑组织，同时取正常蓝斑组织作为对照，应用尼氏染色观察蓝斑形态，采用免疫组织化学、免疫印迹和RT-PCR方法分别观察及检测各组蓝斑神经元GR表达的变化，并进行图像分析和统计学处理。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失