组织化处理的

After Bel-7402 was treated with melittin, detection methods were applied as followed, 1 measure the change of proliferation by MTT ; 2 light microscope and transmission electron microscope were used to observe the morphous and biological behaviour and ConA agglutination test and clonality test were applied to measure the adhesion capability and growth potency of Bel-7402; 3 ELISA , colorimetry , bromocresol green mehod were used to determine AFP level, the activity of y - GT and ALP, ALb level respectively; 4 the change of c-myc gene expression was detect by immunohistochemical method.

观察蜂毒素作用于人肝癌Bel-7402细胞株后(1)MTT方法测定对肿瘤细胞增殖的影响(2)光学显微镜和透射电镜下观察对形态及生物学行为的影响，ConA凝集试验及克隆形成能力反映处理后细胞粘附能力及生长潜能(3)ELISA法测定甲胎蛋白含量、重氮反应比色法测定γ-谷氨酰转肽酶活力、比色法测定碱性磷酸酶活力、溴甲酚绿法测定白蛋白含量，检测对肝癌细胞代谢与分泌的影响(4)免疫组织化学法检测处理前后c-myc基因表达的差别。

After treatment with 50 mmol·L-1 crocin, the inhibited growth of tumor was observed. Electronic microscope was used to observe the morphological changes. The expressions of Bcl-2, Bax, Survivin and Cyclin D1 were detected by immunohistochemistry.

随机分为对照组和处理组。50 mmol·L-1 crocin处理后，观察其对肿瘤的抑制作用；电镜观察肿瘤组织形态改变；SP-免疫组织化学法分析Bcl-2，Bax，survivin，Cyclin D1蛋白表达。

In the pretreatment group, Bak Foong Pill extracts were given for 14 days prior to MPTP. Immunostaining of tyrosine hydroxythase was used to observe the damage of dopamine neurons, and of Bax to detect the protein levels of Bax. High performance liquid chromatography-electrochemical detection was used to investigate the contents of DA and its metabolites dihydroxy-phenylacetic acid and homovanillic in the Str.

给予BFP预处理后，用酪氨酸羟化酶免疫组织化学法观察黑质神经元的变化；同时应用免疫组织化学法检测Bax蛋白表达；应用高效液相-电化学方法检测纹状体巴胺及其代谢产物二羟基苯乙酸和高香草酸的含量变化。

In the pretreatment group, Bak Foong Pill extracts were given for 14 daya prior to MPTP. Immunostaining of tyrosine hydroxythase was used to observe the damage of dopamine neurons, and of Bax to detect the protein levels of Bax. High performance liquid chromatography-electrochemical detection was used to investigate the contents of DA and its metabolites dihydroxy-phenylacetic acid and homovanillic in the Str.

给予BFP预处理后，用酪氨酸羟化酶免疫组织化学法观察黑质神经元的变化；同时应用免疫组织化学法检测Bax蛋白表达；应用高效液相－电化学方法检测纹状体多巴胺及其代谢产物二羟基苯乙酸和高香草酸的含量变化。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

The thesis thinks the present conditions and characteristics of appeals for help involved in lawsuit are that appellants are nature persons primarily, appealing causes are to refuse to accept decisions and reflect to execute improperly, cases type are civil cases primarily, as well as showing those characteristics that appeal case are getting long-term, group appeal are getting organizing and appeal action are getting non-rationalizing and so on from the angle of a judge who is engaged in the administration of justice, and on the basis of relevant data which are a case of appeals for help involved in lawsuit of two level courts and centralized dealing ways of autonomous prefectures.

论文导师刘志坚，论文学位硕士，论文专业法律论文单位兰州大学，点击次数 2，论文页数 59页File Size2592K本文从一个从事审判工作的法官的角度出发，以自治州两级法院涉诉上访案件的个案及自治州涉法上访集中处理的有关资料为基础分析认为涉诉上访的现状及特点是上访人以自然人为主、上访事由以不服判决和反映执行不力为主、案件类型以民事案件为主，同时呈现出上访案件长期化、群体上访组织化、上访行为非理性化等特点。

Through analysis and discussion in the following chapters, four strategies are summarized for teachers' further reference.(1) The individualized physical education program－Through the plan with similar to IEP, teachers arrange physical activities properly for children with mental retardation.(2) Cognitive behavior modification－Through demonstration and guidance, students are able to change behaviors by modeling and correcting their cognitive patterns.(3) Task analysis approach－Teachers have to divide a particular movement skill into several individual sub-skills and then students would be able to master that specific skill by linking every well-practiced sub-skill.(4) Data processing mode－Students are motivated to learn with fun because the target skill has been analyzed into meaningful and organized procedures.

经过分析与探讨，本文提供四大体育教学策略供从事体育教学之教师参考：一、个别化体育计划——透过类似个别化教育计划（Individual Education Program， IEP）的计划，让教师先对智能障碍学童的需求与体育活动作适当的安排；二、认知行为改变技术——教师以示范、引导的方式，使智能障碍学童经由模仿，修正认知，让行为得以改变；三、工作分析法——教师先将动作技能分解成数个分项，智能障碍学童经充份练习每一分项，最后再将每个细部动作串联；四、讯息处理模式——透过教师引发学习动机，并将动作以有意义化与组织化的学习方式，让智能障碍学童从乐趣中学得动作技能。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失