组织化

Methods The target DNA sequence of yrdC was obtained from human spleen tissue by using RT-PCR,construct high titer recombinant adenovirus Ad-yrdC by using AdEasy adenovirus carrier system,detect yrdC protein expression by using Western blot and immunohistochemical method,set up control group and transfected Ad-Null group,and study the effects Ad-yrdC on BGC-823 cell line by drawing cell growth curve and MTT chromatometry.

方法用RT-PCR方法获取人脾脏组织yrdC基因序列，采用AdEasy腺病毒载体系统构建携带yrdC基因的高滴度重组腺病毒Ad-yrdC，将其转染胃癌BGC-823细胞，应用Western blot和免疫组织化学法检测yrdC蛋白表达，设对照组和转染Ad-Null组，并分别绘制细胞生长曲线和MTT比色法研究Ad-yrdC对BGC-823细胞的影响。

The level of serum amylase, the pulmonary dry/wet weight ratio and histological changes were measured at different time points in each group. The expression of pulmonary CINC protein and mRNA were detected by immunohistochemistry and semi-quantitative reverse transcriptase polymerase chain reaction, respectively.

分别检测各组不同时间点血清淀粉酶、肺干湿重比和肺组织病理学改变，用免疫组织化学法和半定量逆转录聚合酶链反应法检测肺组织中CINC蛋白和CINC mRNA表达的变化情况。

METHODS: The acute pancreatitis model was induced by retrograde injection of 50 g/L sodium taurocholate into the bili-pancreatic duct in SD rats. Forty-eight mas-culinity SD rats were randomly divided into 2 groups: the sodium taurocholate group and the operational control group. The serum amylase level and the histol-ogical pathologic changes of pancreas were measured at different time point in each group. The expression of pancreatic CINC protein and mRNA were determined by immunohistochemistry and semi-quantitative reve-rse transcriptase polymerase chain reaction, respectively.

采用向胆胰管逆行注射50 g/L牛磺胆酸钠建立大鼠SAP模型， 48只♂SD大鼠随机分为手术对照组和SAP组，分别检测各组不同时间点血清淀粉酶和胰腺组织病理学改变，用免疫组织化学法和半定量逆转录聚合酶链反应法检测胰腺组织中CINC蛋白和CINC mRNA表达的变化情况。

The developmental press model quickly metamorphosed into an authoritarian one as state co-optation and control of the media became more institutionalized.

发展上的杂志报纸模型进入 authoritarian 之内很快地使变形一如州选举，而且媒体的控制变成更多组织化。

Along with economic globalization unceasingly thorough, drug-related crime to organization, collectivization development.

随着经济全球化的不断深入，毒品犯罪正在向组织化、集团化发展。

The last part of this thesis is to describe and standardize the organized information and interface by computer language.

研究的最后是对组织化的信息及接口采用通用的计算机语言进行描述和标准化。

METHODS: The expression of COX 2 and VEGF in 32 cases of endometriosis patients was detected by immunohistochemistry methods.

采用免疫组织化学法对32例EMs患者组织中COX 2和VEGF的表达进行检测。

Seeking for knowledge are to cram our brain with a lot of knowledge and then systemize and sort them out. The knowledge is expected to use widely in daily life. So, the more we learn; the wider we search.

求知识、做学问是将一大堆知识想尽办法地纳入一个人的大脑之中，将之系统化、组织化，以期在日常生活中将之广为运用，因此愈学愈多，愈搜愈广。

Results Administration of L-NAME could attenuate the activity of NOS and inhibit spermatogenic cells apoptosis in rats experimental cryptorchidism, and it could significantly inhibit weight loss of the cryptorchid.

手术建立大鼠单侧隐睾模型，实验分为隐睾组、隐睾+L-NAME组［术后腹腔注射溶于生理盐水的L-NAME 50 mg/(kg\5d)］、隐睾+生理盐水组、假手术组，7 d后采用化学比色法检测睾丸组织NOS活性，流式细胞术和原位缺口末端标记法检测生精细胞凋亡，RT-PCR和免疫组织化学法检测Bax基因表达变化。

HP were determined by rapid urease test,Warthin-Starry silver stain and polyme rase chain reaction,and expression of P21,P53,C-myc and C-erbB-2 were detecte d by immunohistochemicology (Streptaovidin/Peroxidase,SP) method,and were evalua ted with positive expressive integrality,the mean optical density an d the integral optical density in computer assisted image analysis system.

方法经内镜和病理诊断的胃癌91例，用快速尿素酶试验，改良W-S银染色和PCR方法检测上述标本中Hp；以S-P免疫组织化学法检测P21、P5 3、C-mcy及C-erbB-2，并用阳性表达积分半定量和免疫组化图像分析进行比较。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失