组织化

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

The existence of the chimaeric gene, TA29-366/GUS/NOS-T, in each kanamycin resistant tobacco plant was demonstrated by PCR and Southern analysis. The histochemical analysis of GUS activity showed that the GUS was appeared only in the tapetal cells of tobacco anther.

以组织化学法分析GUS酵素的活性，证实GUS在TA29起动子的调控下，仅在转殖菸草花药的营养层细胞表现，而在叶子等其他组织并不表现。

Methods The expressions of Tiam 1 and PCNA in cancer tissues and normal gastric mucosa of 60 cases with gastric cancer were detected by immunohistochemistry method.

采用免疫组织化学法对60例胃癌组织及其相应正常胃黏膜组织标本进行检测。

Objective To observe expression of T lymphoma invasion and metastasis inducing factor 1 (Tiam 1) and proliferating cell nuclear antigen in gastric cancer and investigate their relationship with clinicopathological parameters.

目的探讨T淋巴瘤侵袭转移诱导因子1(Tiam1)和增殖细胞核抗原在胃癌中的表达及其与临床病理参数的关系。方法采用免疫组织化学法对60例胃癌组织及其相应正常胃黏膜组织标本进行检测。

We measured the expression of P-gp、GST-π、TopoⅡin 80 specimens by immunohistochemical technique.

采用S-P免疫组织化学法检测80例乳腺癌组织中P-gp、GST-π和TopoⅡ的表达情况。

Under the household contract management system, beacouse of the low systematism level, individual peasants are unadvantageous position in the gaming with low-level governments and market organizations.

内容提要家庭经营制度下的个体农民由于组织化程度较低，在与基层政府、市场组织等乡村社会各利益主体的博弈中处于较不利地位。

The HSS action showed a dose-effect manner and this action was proved uneffective with the larger dose of CCl〓.(2) Hepatic histological findings indicated that CCl〓-induced hepatic lesions could been alliviated by HSS.(3) CCl〓-induced reduction of hepatic mitochondrial succinic dehydrogenase activity was restored by the treatment of HSS.

相反，如果CCl〓所用剂量过大，HSS将无抗损伤作用；(2)HSS可使CCl〓损伤肝组织的程度减轻，用肝组织切片可充分证明这一现象；(3)HSS可使CCl〓损害肝细胞线粒体琥珀酸脱氢酶的活性恢复，用组织化学法可得到证实。

A continuous increase in FS increased L value, A-lys, SF, pressure and torque; but decreased TI, NSI, WHC and OAC; chewness increased from 4r/min to 8r/min, but decreased rapidly when FS raised from 12r/min to 16r/min, there was a little change between 8r/min and 12r/min, there was also changed unsignificantly between 16r/min and 20r/min.

随着喂料速度的升高，产品的亮度指数、有效赖氨酸含量、剪切力、压力和扭矩逐渐升高；组织化度、氮溶解指数、水分保持能力和吸油性逐渐下降；当喂料速度由4r/min升高至8r/min时，咀嚼性逐渐升高，由12r/min升高至16r/min时，咀嚼性迅速降低，8r/min与12r/min之间变化很小，16r/min与20r/min之间变化很小。

Methods The expression of XIAP in sixty cases of TCCB and ten cases of nontumorous urocystic tissue were detected by RT-PCR and immunohistochemistry.

方法应用RT-PCR和免疫组织化学法，检测XIAP在60例膀胱移行细胞癌和10例非肿瘤膀胱组织中的表达情况。

Methods: Forty-four specimens of renal tissues were harvested from urolithic patients,control tissues were harvested from non-urolithic patients (6 specimens) and renal tumorpatients performing nephrectomy (21 specimens). Immunohistochemical technique wasestablished to study the location of VKORC1 in renal tissues. Western blot technique wasused to detect the differential expression of VKORC1 protein in renal tissues.

方法收集肾脏皮质组织标本71例，其中草酸钙结石组44例，对照组27例(肾盂输尿管交界处狭窄和输尿管结核组6例，皮质厚度小于1.0cm；肾肿瘤皮质组21例，皮质厚度大于1.0cm)，应用免疫组织化学法检测VKORC1在组织中的表达部位，应用免疫印迹蛋白半定量法，检测各组VKORC1蛋白的表达，并分析其表达与肾脏积水及皮质厚度等因素的关系。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失