组织化

Methods: 9 cases of exogenic OVC、6 cases of cystoid and infiltrative OVC、15 cases of well-differentiation OSCC and 15 cases of poor-differentiation OSCC were selected; two specimens, which were carcinoma tissues and the sur...

方法取9例外生型口腔疣状癌、6例囊肿／浸润型口腔疣状癌、15例高分化鳞癌、15例低分化鳞癌，应用半定量逆转录多聚酶链式反应方法检测口腔疣状癌和口腔鳞癌中OPN mRNA的表达水平，应用免疫组织化学法检测上述标本中OPN的表达及分布，判断差异。

Cases healthy subjects served as control. In addition, density of glomerular matrix membrane and quantity of cell in glomeruli were measured with CMIAS image analysis system by computer. Global sclerosis, cellular crescents, fiber or fibrocellular crescents were measured with Memphis scope. Tubulointerstitial lesions were examined with semi-quantitative grades (including O-III grades).Results Compared to normal control, there was a significant increase of urinary TGF-betaK IL-6 and Col-lV levels in patients with IgAN (p. 01). There were also significantly positive correlation between the levels of urinary TGF-betaK Col-IV and density of glomerular matrix membrane ., interstitial fibrosisp.

采用酶联免疫吸附法测定尿TGF-β1(Transforming growth factor-β1)、尿IL-6(interleukin-6)和尿Col-Ⅳ(type Ⅳ Collagen)；采用免疫组织化学法检测肾组织TGF-β1、Col-Ⅳ表达；应用CMIAS多功能真彩色病理图像分析系统对肾小球基质基底膜面密度和细胞个数进行半定量测定；对球性硬化、节段硬化、细胞新月体及纤维或纤维细胞新月体所占肾小球百分数和肾小球IgA沉积免疫荧光强弱的判断采用Memphis法进行评分；肾小管间质病变程度采用光镜下半定量分级法(包括0-Ⅲ级)。

The animals were sacrificed 55 minutes later, then immunohistochemical methods were used to show the change of nNOS in hippocamp.

采用免疫组织化学法，观察中药复方对戊四唑致痫小鼠nNOS表达的影响。

Methods Immunohistochemical staining was used to detect the expression of p53,p27 and mdm2 in 68 cases of GIST.Their relations with histomorphology and biological behavior were analyzed statistically.

收集68例手术切除的GIST标本，应用免疫组织化学法检测肿瘤组织中p53、p27和mdm2蛋白的表达，并结合病理形态学及生物学行为评价进行统计学分析。

Methods:Animal models of 25 adult mixed breed dogs were established by transverse osteotomy at bilateral iliopectineal crest,which were fixed with ATMFS anterior column connector and steel plate respectively. The animals were examined at 2,4,6,8,and 12 weeks by X-ray,macropathological observation and biomechanical test. Tetracin fluorescent labeling,HE staining,Masson triad colour staining,in situ hybridization and immunohistochemical method were performed and histological image analysis were applied.

建立犬双侧骨盆弓状线骨折的动物模型，分别采用ATMFS前柱固定器和5孔重建钢板内固定，于术后2、4、6、8、12周分别行影像学检查、大体观察及生物力学测试；采用四环素荧光标记法、HE染色、Masson三色染色法、原位杂交法、免疫组织化学法进行组织学观察并图像分析；采用RT-PCR法检测骨折端不同时期骨钙蛋白和核心转录因子mRNA相对表达量的变化。

It is an objective psychological guarantee system in view of psychology, is a result of utilitarian calculations, and an institutionally and structurally operative system in view of managerial science.

可以这样认为，契约型政府信任关系是在对社会制度和治理秩序的主观设计和建构中历史生成的，从哲学的角度看，它经历了一个从怀疑主义到契约论的过程；从心理学的角度看，它为与国家二元分立中的社会提供了一种客观性的心理保障机制；从经济学的角度看，它是个体功利性计算的集体选择；从管理学的角度看，它的制度化具有组织化的可操作性。

Methods: Thirty cases of gastric cancer were collected from June to October, 2007.The anterior lobe of transverse mesocolon biopsy was undertaken intraoperatively, CK19、CK20 im munohistochemical staining were performed on the tissue from biopsy, compared with HE staining and peritoneal lavage cytology.

2007年6月—2007年10月期间30例行手术治疗的胃癌患者，术中行横结肠系膜前叶活组织检查，免疫组织化学法检测CK19及CK20的表达，并行HE染色，以腹腔灌洗检查癌细胞作比较。

Methods: The distribution of nitric oxide synthase-containing neurons in the gastrointestinal tract of the Microtus mandarinus was studied with NADPH-diaphorase histochemistry.

用NAD-PH-黄递酶组织化学法对NOS在棕色田鼠胃肠肌间神经丛的分布进行比较研究。

Methods:Immunohistochemical technique was used to detect the p21 WAF1/CIP1and P53 protein expressions in:20 usual leiomyoma,70 borderline leiomyoma:including 40 cellular leiomyoma,13 bizarre leiomyoma,4 mitotically active leiomyoma,10 atypical leiomyoma, 3 smooth muscle tumors of uncertain malignant potential and 18 leiomyosarcoma...

应用免疫组织化学法检测 2 0例普通型子宫平滑肌瘤，18例子宫平滑肌肉瘤及 70例交界性子宫平滑肌瘤：包括 4 0例富于细胞型子宫平滑肌瘤、13例奇异型子宫平滑肌瘤、4例核分裂活跃型子宫平滑肌瘤、10例不典型子宫平滑肌瘤及 3例恶性潜能未定型子宫平滑肌瘤的p2 1WAF1/CIP1和P5 3蛋白的表达。

Under new situations,they appear some flew characteristics in the organized crimes:the number is increasing with a rapid growth;the character is developing more typical towards underworld;the equipment used in the crimes is becoming moderner;the degree of systematization is turning higher and higher.The tendency towards enterprise is changing dearer and clearer;the means is becoming softer and softer;they appear territorial and international tendency.

在新形势下，有组织犯罪呈现出一些新特点：带黑社会性质的犯罪数量上迅速增长，质量上正向典型的黑社会犯罪演化；装备更趋现代化；犯罪的&组织化&程度越来越高，&企业化&趋势更加明显；犯罪手段上越来越重视&软&手段的运用；犯罪活动呈现跨区域化、国际化的发展趋势。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。