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组织切片

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Methods The third toes were sectioned by histological method and observed under microscope.

方法用30例成人第三趾经组织学处理切片,借助于显微镜进行观察。

All mice were killed by cutting neck on 7th day,ulnas and femur were taken out.The bone marrow histologic morphology and ultrastructure of mice ulnas were ovserved.

于第7天断颈处死小鼠,取出尺骨和股骨,计数骨髓单个核细胞,并行尺骨组织学切片和超微结构观察。

The ulnas of fetal mice were cultured for 48 hours in BGJb medium treated respectively with estradiol and purearin. After fixing long bones and decalcification, paraffin sections were made, and then performed immunohistochemical staining for determining the level of typeⅠcollagen protein in growth plates. Positive cell locations in the resting zone, proliferative zone and hypertrophic zone of epiphyseal plate were observed under light microscope.

剥离出胎鼠尺骨,置于BGJb培养基中,葛根素和雌二醇作用48 h后,将长骨固定,脱钙后作石蜡切片,进行免疫组织化学染色,光镜下观察骺板静止区、增殖区和肥大区阳性细胞定位,图像分析系统下测定免疫反应阳性细胞面积。

Pathologic studies on animal liver dissection reveals significant effect of the bioactive antioxidant of Young Barley Grass Powder in the protection of the liver from acute liver damage or chronic liver disease.

而在实验动物病理组织的切片上也发现,抗氧化活性成分不论对急性肝损伤或慢性肝病变,都有保护的功效。

After 1and 2 weeks, the cervices of mice of different groups underwent histophathologic examination.

经1~2周后,各组小鼠分别做宫颈切片,进行病理组织学检查。

After 1and 2 weeks, the cervices of mice of different groups underwent histophathologic examination.results cervicitis occurred in more than 90% mice of the vegina mechanical stimulation group.

经1~2周后,各组小鼠分别做宫颈切片,进行病理组织学检查。结果阴道机械刺激组90%以上小鼠宫颈产生炎症。

Diseased cobias were collected from some net-cage farms at Hsiqu Liouchiou Island, next to Dunggang, Taiwan. Some little nodes appeared on surfaces of their fins and skins. When the symptom became serious, they looked like sarcomas. Almost every organ, including liver, spleen, kidney, fin, heart, stomach, intestine of a hard-sick cobia were dealt through the following processes:fixation, section and staining. Only at the connective tissues of fins and epithelium did we find hypertrophy cells which own big nuclei and inclusion bodies around the nuclei.

中文摘要本实验自屏东小琉球采集某家养殖业者箱网网中的罹病海鲡,外观上,鳍与部分体表出现结节状之小颗粒,严重时,呈现瘤状肉柄,取其各部位组织包括:肝脏、脾脏、肾脏、鳍、心脏、胃、肠等进行固定、切片及染色,发现唯有於鳍与皮肤部位之结缔组织细胞,明显可见肥大之现象,肥大之细胞拥有巨大细胞核与围绕著细胞核的包涵体网状结构,由於肥大细胞之堆叠,导致鱼体之鳍与一些表皮部位有颗粒状的「结节」存在。

Methods Both sides of the condyles of femur from 3 female sheep with ovariectomy-induced osteoporosis sine employed in this experiment.

3只经去势后骨质疏松的绵羊选取双侧股骨髁,植入EPS,饲养3个月依次进行切片组织学观察。

Anatomy study on the protocorm-like bodies formation and development of Cymbidium hybridium was conducted.

以大花蕙兰类原球茎发生发育不同阶段的培养物为试材,采用石蜡切片法对其进行组织学研究。

The rats were killed by cutting theirnecks and physical blood was taken for preparing serum, interleukin 2 (IL-2) was detected by radioimmunoassay ; the spleen was taken for measuring ConA-induced reaction of lympho- cyte proliferation and induced production of IL-2; and the activity of induced production of spleen's IL-2 was determined by the means of cell proliferation of mouse thymus; the skin and trachea were taten for slice with paraffin wax, dyeing with methybenzoamine blue so as to survey mast cells .

将大鼠断头留取躯干血制备血清,用RIA法检测白细胞介素2(IL-2);取脾脏行ConA诱导的淋巴细胞增殖反应测定和IL-2诱生;并用小鼠胸腺细胞增殖法检测脾IL-2诱生活性;取皮肤和气管做石蜡切片、甲苯胺兰染色检测肥大细胞,结果显示:应激组较对照组脾淋巴细胞增殖降低,cpm分别为9466±3.17和24019±3879(P.001);MC明显减少,分别为8.6±3.0/HP和25.4±3.7/HP(P.001),前组形态异常较其甚(MC多较小,呈多角或不规则状),且组织中可见分散的崩解颗粒。

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呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

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