组织切片

Results:(1) The 90Y activity was higher while the contumination of 90Sr was less than 0.0031%;(2) The efficiency of 90Y-labeled antibodies was over 90%,the activity of labeled antibody maintained 82.3%;(3) Tumor size of all 90Y-antibody-treated model was significantly less than that of all 90Y-Cl3-treated model, the average survival time of the mice of the 90Y-antibody-treated group was significantly longer than that of the 90Y-Cl3-treated group;(4) In the tissue sections extensive necrosis was observed in all 90Y-antibody-treated tumor, the living tumor cells were immunohistochemically stainable with PcNA.

结果：(1)EDTA淋洗法制备90Y纯度高，其相对含量可达99.997％；(2)单克隆抗体的90Y标记率达90％以上，抗体蛋白活性保持82.3％；(3)治疗结果表明治疗组肿瘤明显小于对照组，原肿瘤的指数生长规律遭到破坏，治疗组生存期明显长于对照组；(4)组织学切片显示治疗组肿瘤有广泛坏死，PcNA免疫组化显示治疗组肿瘤细胞增殖受到明显抑制。

The structure and 5-HT immunoreactivity in the brain and suboesophageal ganglion of three beetle s, Ambrostoma quadriimpressum, Henosepilachna vigintioctomaculata and Oxycetonia jucunda, were first studied by means of colophony-paraffin embedding serial section technique and strepteavidin-peroxidase immunohistochemical method.

采用树脂石蜡组织包埋连续切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶染色法，首次对三种甲虫(榆紫叶甲、小青花金龟、马铃薯瓢虫)的脑进行了详细观察研究，分析了三种甲虫脑组织结构特征，初步构建了三种甲虫脑内5-HT抗原系统免疫组化反应模式。

The structure and 5-HT immunoreactivity in the brain and suboesophageal ganglion of three beetles, Ambrostoma quadriimpressum, Henosepilachna vigintioctomaculata and Oxycetonia jucunda, were first studied by means of colophony-paraffin embedding serial section technique and strepteavidin-peroxidase immunohistochemical method. The results showed that the brains of these three taxonomically closely related beetles were remarkably different in composition and size.

采用树脂石蜡组织包埋连续切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶染色法，首次对三种甲虫(榆紫叶甲、小青花金龟、马铃薯瓢虫)的脑进行了详细观察研究，分析了三种甲虫脑组织结构特征，初步构建了三种甲虫脑内5-HT抗原系统免疫组化反应模式。

At 1st、2nd、3rd months after surgery the rats were killed respectively and the implanted tibiae were got , the tissue samples were fixed with 10% Formaldehyde soaked and 8% formic acid was used to dissolve the calcium for 10 days.

于种植术后1个月、2个月、3个月时，取出带种植体的胫骨，取下的标本立即放入10%甲醛缓冲溶液固定、经过8%甲酸溶液脱钙、梯度酒精脱水后，制作HE染色的组织学切片，做光镜及骨组织的形态计量学观察。

We use a dog femoral shaft transcortical implant model in animal experiment. Bioactive hydroxyapatite, porous and without coating implants were implanted in 10 dogs.

动物实验采用犬股骨干横穿皮质骨模型，10只实验犬，分别植入三种不同假体，在植入后2.4.6.8.12周分批收集标本，利用X线摄影，不脱钙骨切片组织学观察。

The samples were prepared and stained with modified Mallory's trichrome staining and observed under light microscope.

分离含有牙胚的上、下颌骨，固定脱钙，25 g/L火棉胶、石蜡双重包埋，常规组织学切片，分别用改良Mallory's三色和HE染色，镜下观察。

In functional study, overexpression assay was conducted in potato. Interestingly, tuberous swelling along roots was observed in some transgenic potato lines in addition to stem-derived tuber formation. Conclusively, we propose a hypothesis to illustrate the mechanism by which IbMADS1 participates in the initiation of tuberization in sweet potato.

利用解剖切片观察并比较转殖马铃薯与野生型甘薯的根部块状膨大区域，可以发现两者在膨大初期，中柱内含的细胞数皆增多，尤其是木质部细胞出现不正常增生情形，进而扩展中柱区域；因此，我们可以初步推论在甘薯块根发育初期，IbMADS1藉著作用於根部分生组织，尤其是中柱的原始形成层，从而促进细胞分裂，并参与甘薯的初期结薯。

After administration of L-N〓-nitro-Arginine, a specific antagonist of NOS, it was found that the basal and stimulated NO release of blood vessels (aorta, internal carotid, renal and mensenteric artery) was decreased further. The increase of coronary blood flow stimulated by Ach was also reduced further, while the platelet aggregation degree and erythrocyte aggregation index increased significantly. Pathological examination indicated that aortic endothelium was destroyed, the blood vessels in cerebral, cardiac and renal tissues were hypertrophied further and some were almost occluded. The above fin dings resulted in SHR feeded with L-NNA having high incidence of stroke rate and high degree of renal cirrhosis when compared to control SHR. The infarct and hemorrhagic focuci in cerebral tissues, infarct focuci in cardiac tissues, atrophy and hyalinedegeneration of renal glomeruli and degeneration even necrosis of renal tubuli in renal tissues were al so found. The increase ratio of brain and heart to body weight and decrease ratio of kidney to body wei ght further demonstrated that the cerebral, cardiac and renal injuries were aggravated.

在上述结果的基础上进一步给予SHR NOS特异性拮抗剂左旋亚硝基精氨酸后，血管(胸主、颈内、肾及肠系膜动脉)NO基础释放及激动剂刺激释放进一步减少，Ach刺激冠脉流量的增加和胸主动脉内膜NOS活性也进一步减少，血小板聚集程度及红细胞聚集指数显著上升，病理切片显示胸主动脉内皮细胞破坏明显，脑心肾组织内的血管进一步增厚甚至几乎阻塞，导致饲L-NNA的SHR缺血性及出血性脑卒中发生率显著高于对照SHR，脑体重比显著升高；心肌内出现明显的缺血梗塞疤痕灶，心体重比显著升高；肾小球明显萎缩、玻璃样变性，肾小管也变性甚至坏死，使肾硬化程度显著增加呈颗粒性固缩肾，肾体重比也显著减少。

Methods The cochlear sections of the guinea pigs exposed to experimental blast underpressure were observed using light microscope.

成年豚鼠暴露于不同强度的实验性冲击波负压后，光学显微镜下观察耳蜗的组织学切片病理变化情况。

The quantity and appearance of Foslike immunopositive neurons in ventrolateral part of PAG were investigated by immunocytochemical staining method.

采用免疫组织化学ABC法对大鼠中脑冰冻切片进行染色，显微镜下进行观察并拍照，对Fos免疫阳性细胞进行形态与计数分析。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。