组织切片

METHODS: The suspension of bletilla particles or bletilla gum was injected into the right internal carotid artery after the external carotid artery was exposed in Wister rats. In a certain period after operation, the assessment of neurological defects, red tetrazoline staining and HE staining were performed.

手术暴露大鼠右侧颈部血管，向右颈内动脉注入一定量的白芨微粒悬液或白芨胶，在术后一定时间内进行神经功能缺失评分、大脑切片红四氮唑染色和组织学H.E染色。

Pathology studies of the bronchial biopsies revealed endobronchial chondroid hamartoma.

经由切片病理组织报告此肿瘤为一软骨性过误瘤。

The model mice were then treated by giving GDNF into the striatum. After a seven-day survival period, the model mice were sacrificed to get the segment of substantial nigra fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry using anti -TH and anti-CB antibodies to label DA neurons and CB-containing neurons respectively, which were counted under microscope and analyzed statistically.

利用1-甲基－4-苯基1，2，3，6-四氢吡啶(1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine，MPTP)制备成年小鼠PD模型，通过单侧纹状体定位注射GDNF，取中脑黑质节段，做连续冠状石蜡切片，结合免疫组织化学染色方法，观察各组酪氨酸羟化酶(tyrosine hydroxylase，TH)、钙结合蛋白(calbindin D28k，CB)的表达，光镜观察并细胞计数，统计学分析。

After fixation,the brain was cut coronally into four blockes:the middle partof cerebrum,midbrain,pons and medulla oblongata.These blockes were embeddedroutingly and serial 8μm sections were prepared for HE,specific histochemical andimmunohistochemical stainings.

所有脑组织经固定后冠状切取大脑中部、中脑、桥脑及延髓4个组织块，常规包埋，连续切片（8μm）分别作HE、组化及免疫组化染色。

Cytohistological observations of embryogenic callus of litchi and longan were carried out by paraffin slice.

通过石蜡切片观察荔枝、龙眼胚性愈伤组织的结构和胚性细胞分裂生长的方式。

We herein report a male newborn infant presented with an erythematous and ulcerated mass on his lower back at birth and was found to be a malignant rhabdoid tumor, diagnosed by histopathologic and immunohistochemical studies.

我们报告一位男婴，出生在下背部发现一个红色、表面溃疡的皮肤肿块。经皮肤切片以及免疫组织化学诊断为类横纹肌肉瘤。

Methods: The expression of BRCA1 protein in 72 cases of breast cancer tissues and 15 cases of breast fibroadenoma tissues was examined by immunohistocbemical method.

采用免疫组化SP法对72例乳腺癌（35例朝鲜族，37例汉族）、15例乳腺纤维腺瘤组织石蜡切片进行BRCA1检测，分析与临床病理特征的关系。

In this research, the pubertal five-week-old SD rats were chose as experimental object to establish the animal model for simulating maxillary protraction, on which was imposed the orthopedic force of 85g with utilizing the self-devised maxillary protraction appliance. Located lateral X-ray cephalograms were taken to measure and analyze the changes of maxillary development after exerting the orthopedic force for 4 weeks. The technique of immunohistochemistry was used to investigate theexpression of TGF-, in frontomaxillary sutures and palatomaxillary sutures with identical force performing for different extent of time. The average hue was selected as indicator, which was measured by the means of the analysis system of pathological color images. The measuring data were analyzed by the one-way ANOVA in software SPSS 11.0 version.

本研究采用自行设计的上颌前牵引装置，以生长发育期5周龄SD大鼠为实验对象，施加85g的矫形力，建立了模拟上颌前牵引的动物模型，通过X线头影测量分析，观察施力4周后上颌骨的生长发育情况；应用免疫组织化学技术，检测同一力值作用不同时段下TGF-β_1在上颌骨的额颌缝、腭颌缝的表达，彩色病理图象分析系统测定每张切片中阳性染色细胞的平均灰度值，结果采用SPSS 11.0进行方差分析。

Methods The unfixed tissue blocks were put into the freezing chamber and cut it for 5 or 10 μm section at once after it were frozen,then put it into the fixactives for 20 seconds and stained with haematoxylin and eosin.

将未经固定的组织块放入冷冻箱内，冷冻后切片5μm或10μm，即刻于固定液里固定20s，用HE染色。

The steps are as the following:1、Preparing the cerebrospinal fluid one hour before the experiment,take some of them for cryopreservation;2、Without paralyse,cuting down the rat head quickly and dislodge the hippocamp part which is used in the experment in freezing liquid,and trim the hippocamp distrct.3、Fixing the hippocamp tissue on the vibratome,to cut down a brain slice with 300μm,use the haustorial tube to move the slice to the preincubate dish,cultivanting it for one hour.Then move it to the record incubation chamfer.4、Preparing the glass micloelectrode and fill it with NaCl,the corcentation is 3mol/L.5、adjusting the recorder,after cultivate the brain slice in the incubation chamfer for 2 hours,the expermentize can be gone on.6、In order to ensure the accurate of the experimental result,use only one medicine concentration for one brain slice in every experiment.

本实验研究方法是：1、实验前1小时配制好所需脑脊液并充以95% O2和5% CO2的混合气，取小部分冷冻保存；2、在乙醚麻醉下，快速断头并在冷冻液里取出包含海马的大脑部位，修整出所需海马区；3、将海马组织固定于振动切片机上切出300μm厚的海马脑片，用广口吸管转移至预孵育皿培养一小时，后再转移到记录用孵育槽内；4、拉制好玻璃微电极，并充以3mol/L的NaCl；5、调试好记录仪器，将脑片在孵育槽内培养2小时后进行实验；6、为了保证实验结果的准确性，每一块脑薄片只进行一个浓度的药品实验。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。