组织切片

Methods Healthy male SD rats of different age groups were recruited(1-, 2- and 3- week groups, 1- and 2- month groups, 6- month grown group, and 18- month aged group; n=5 each). Immunohistochemistry was performed on the coronal sections of brain of the rats perfused with paraformaldehyde.

健康雄性SD大鼠，分为7天、2周、3周、1月、2月、成年（6月龄）和老年（18月龄）7组，每组5只，共35只，多聚甲醛灌注固定，冰冻冠状切片，免疫组织化学染色，光镜观察摄像，细胞计数。

Cell survival and differentiation were studied with immunohistochemistry, Prussian blue staining, and HE staining.

采用免疫组织化学、普鲁士蓝和HE染色对脑组织切片进行干细胞存活、分化及病理学研究。

Cobra venom factor was given to prevent hyperacute rejection and FK506 was given to prevent cellular rejection. The xenograft recipients were killed and cardiac xenografts harvested 4, 8, 12, 16, 24 hours after transplantation. Histologic examination was used to demostrate the degree of rejection and coagulation while revers transcriptase-polymerase chain reaction was used to detect the expression of tissue factor mRNA, and the guinea pigs hearts, harvested but not transplanted, were studied as controls in all samples.

建立豚鼠－大鼠异种异位心脏移植动物模型，使用CVF抑制超急性排异反应，FK506抑制细胞免疫排斥反应，分别在移植术后4、8、12、16、24h切取移植心脏，通过病理切片观察排异反应和凝血强度，同时通过RT-PCR方法检测组织因子mRNA表达强度，未移植豚鼠心脏作对照。

muscles biopsy of 25 patients with lipid storage myopathy were freezed and cut into slices. Specimens by routine histology and acid or alkaly myosin ATPase, oil red O and PAS stain were observed the difference of types of keletal muscle fibers by ATPase stain and degree of fatty and glycogen dispersed in sarcoplasm,explored substancs metabolite .

方法将25例LSM患者活检骨骼肌作为研究材料，做冰冻切片，采用常规组织学和酸、碱预孵育肌球蛋白ATPase法、油红O法、PAS法观察各型肌纤维ATPase染色差别及肌浆内糖原和脂肪的分布，以探讨其物质代谢。

Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats. After 1 and 2 weeks, rats were sacrificed. Adjacent sections were doubly stained by BrdU/microtubule-associated protein 2 (MAP-2) and BrdU/glial fibrillary acidic protein.

通过注射自体动脉血到尾状核制作大鼠脑出血模型，出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4点，1，2周后处死大鼠，相邻脑组织切片行5'-溴脱氧尿嘧啶/微管相关蛋白2和5'-溴脱氧尿嘧啶/胶质纤维酸性蛋白免疫组织化学双染。

METHODS: Cerebral cortex cells of 8-week aborted human fetus were harvested and cultured in vitro to obtain human embryonic NSCs. Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats.

通过注射自体动脉血到尾状核制作大鼠脑出血模型，出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4 点，1，2周后处死大鼠，相邻脑组织切片行5'-溴脱氧尿嘧啶／微管相关蛋白2和5'-溴脱氧尿嘧啶／胶质纤维酸性蛋白免疫组织化学双染。

Toluidine blue staining and typeⅡcollagen immunohistochemistry staining also showed positive results in both groups, but they were more significant in the experimental group.

石蜡切片HE染色可见类似正常软骨的组织学特征，甲苯胺蓝染色及Ⅱ型胶原免疫组化染色阳性，实验组的阳性染色程度较高。

Methods: For 66 cases whose bone marrow showed hypocellularity in BM aspirate smear, trephine biopsy and reticulin staining were done at the same location in the same patient and at the same time as the BM aspirate smear was done.

对66例骨髓涂片显示细胞增生低下患者的同期同部位骨髓活检组织用塑料包埋半薄切片，做常规染色及网状蛋白染色，光镜下观察。

9 cases of tympanum body paraganglioma were observed, the data of their clinical manifestation, digital image, pathology and immunohistochemistry section were analyzed in detail.

分析检查9例鼓室副神经节瘤病例的临床表现、影像资料、病理切片、免疫组织化学检查。

In this paper, the constitution of the variegation and the distribution of different protoplasts in muted fresh tissues of GWG variegated plant of Pelargonium zonal 'Ms.

Pollock'银边绿心型新鲜叶组织为材料，研究斑块结构切片及不同质体细胞的分布，以探索叶斑异化变型机制。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。