组织切片

Then the mice were killed in different time after given the medicine wine, and the wounded limbs were got follow the articular genua, and the limbs were put in the 10% formaldehyde solution to fix them, then the histopathologic sides were done, and the change in the tissue structure were observed in light microscope.

于给药后不同时段分别处死小鼠，然后沿膝关节部位剪取小鼠的击伤腿置于10 %甲醛溶液中固定，作病理组织切片，光镜下观察组织结构形态学变化评价，评价永康宁骨康宁药酒对小鼠击伤腿肿胀有无消肿作用。

Immunohistochemical method: The CD34 monoclonal antibody was selected as the first antibody, with the immunohistochemical SP procedure, then the pathological slice was displayed by DAB and stained again by hematein. One slice was used as a negative control and reacted with PBS fluid(0.01mmol/L) which was the substitute of anti- CD34.(the kits coming from Boster Biotechnology CO.LTD). The number of the vessels and vascular endothelial cells were displayed by immunohistochemical method.

免疫组化方法：病理组织切片以CD34单克隆抗体作为第一抗体，按照免疫组织化学SP步骤进行，DAB显色，苏木素复染，每组用0.01mmol/l的PBS液替代CD34抗体作阴性对照(试剂购自于武汉博士德公司)，应用免疫组化显示血管，对不同时期的血管生成情况及早期的炎性反映情况进行观察。

Objective The origin and the nature of the Hodgkin and Reed-Sternberg cell of Hodgkins lymphoma has been attracting a lot of medical researchers engaged in studying it,for its character by scattered large atypical cells residing in a complex admixture of inflammatory cells.Great improvement has been made since a new method of isolation of single H/RS cells from a frozen section had been set up by KUppers in 1994,and many studies have approved of the B-cell derivation of H/RS cell.lt has been reported that H/R-S cells might partly be originated from B-cell in our research before,but at the same time,we also found that only 18.8% of H/RS cells express CD20,31.3% of immunoglobulin heavy chain rearrangement have been revealed.

目的 霍奇金淋巴瘤(Hodgkins lymphoma，HL)由于它的恶性肿瘤细胞—H／RS细胞一般只占肿瘤组织的极少部分(不到1％)，且散在分布在背景细胞间，因此对于H／RS细胞的来源和性质研究一直是人们探索的目标。1994年德国科隆大学Kūppers等发展了一种从冰冻组织切片上用显微操作仪挑取单个H／RS细胞的显微切割方法进行H／RS细胞基因分析后，人们对H／RS细胞来源的研究有了突破性的进展，多数支持B细胞来源。

The lung of rats were made into lung print smears and lung sections. Pathological changes in lung tissues was observed after staining with gormori methenamine silver and hematoxylin-eosin.

第9周时剖杀，取肺制作肺组织印片和肺组织切片，分别采用六胺银和苏木素－伊红染色，观察病理学变化。

Pneumocystis carinii cysts were observed in the lung print smear after staining with gormori methenamine silver,and pathological changes in the lung section were observed after staining with hematoxylin-eosin.

3肺组织印片六亚甲基四胺银染色观察卡氏肺孢子虫(Pneumocystis carinii，Pc)包囊，肺组织切片苏木素-伊红(hematoxylin-eosin，HE)染色观察其病理变化。

Finally,experiments of bio-micro-dissection on liver tissue slice are carried out with the tool,and better microdissection result is obtained.

最后，针对肝脏组织病理切，利用该显微切割工具进行超声振动显微切割实验，获得了较好的切割效果，充分验证了生物组织切片超声振动显微切割可行性。

The microscopical structure of the liver tissue were observed by HE staining.

大鼠肝脏组织切片HE染色后，行光镜检查，观察肝组织显微结构变化。

The CNTF and FGF2 positive cell number and the positive area percentage, as well as the number of neuron and neuroglia calculated by immunofluorescence analysis in the spinal cord.

将嗅鞘细胞移植入脊髓半横断SD大鼠，观察术后7天和21天嗅鞘细胞在脊髓内的存活情况，并对脊髓组织切片进行CNTF和FGF2免疫荧光组织化学染色，计数阳性细胞并计算阳性面积百分比。

The results showed that: 1mucosal structure of reticulum and omasum in three groups were all integrated; 2fresh weight and the ratio of fresh weight to body weight of reticulum and omasum raised with the increase of the proportion of forage.

选择9头健康的荷斯坦公牛，随机分为3组，日粮精粗比分别为6∶4、5∶5和4∶6，试验3个月后，通过制作组织切片及电镜观察，测定3组试验牛网胃和瓣胃官发育及组织形态的变化情况。

At the end of feeding for 2, 4, 6 and 8 wk, 6 rats in each group were randomly selected, the serum fasting blood sugar, fasting insulin and the fasting insulin resistance index, and the concentration of IL-18 in serum and liver tissue serosity were measured.

将48只♂SD大鼠随机分为正常饮食组及高脂饮食组，各24只，喂养至第2、4、6、8周末时， 2组各随机抽取6只，抽取门静脉血测空腹血糖、空腹胰岛素，计算空腹胰岛素抵抗指数；检测血清及肝组织匀浆IL-18浓度；光镜检查肝组织切片以评价肝脏脂肪变性程度。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。