组织切片

The histopathological changes were evaluated through light microscope after disarticulation sections H.E.

组织病理学上的变化则经膝关节切片后以伊红性洋苏木素染色於光学显微镜下观察。

Observed the skin 24h,5d and 10d after injection,and maked frozen section from the ultraviolet radiation area of guinea pig skin.Observed migration and ecesis of the BMSCs marked by DAPI in ultraviolet radiation area of guinea pig skin by laser scanning confocal microscope.

注射后24h、5天及10天观察豚鼠皮肤变化，同时取照射区的皮肤制备冰冻切片，激光共聚焦显微镜下观察DAPI标记的BMSCs在紫外线照射区皮肤组织内的迁徙及定居情况。

The expression of complement components was studied with the use of immunohistochemistry on sections of the thickened ein graft.

增厚的移植静脉切片上的补体表达情况通过免疫组织化学的方法进行分析。

Results: The tHey and blood fat level in blood plasma and expression of MCP-1mRNAof aorta on model group is obviously higher than that of control group (P＜0.05);the result of immunohistochemistry shows that the expression of VCAM-1 and MMP-2enhanced, Bcl-2 is lower; aorta slice shows endarterium discontinuation, strippingpartly, smooth muscle cell arrange puffly and chaotic, infiltration of lipoid ofdifferent degree are observed in aorta wall, pathological changes suffusion, morexanthoma cell, plaque protrude lumens. The they and blood fat level in blood plasmaand expression of MCP-lmRNA, VCAM-1, MMP-2 of aorta on intervention group aremuch lower than that of model group (P＜0.05); Bcl-2 of intervention group isstrengthened, and the pathomorphology and immunohistochemistry of aorta showpathological changes are slighter than model group.

结果：模型组，血浆中同型半胱氨酸浓度、血脂水平、主动脉MCP-1mRNA表达显著高于正常对照组(P＜0.05)，免疫组化示主动脉内皮细胞VCAM-1、MMP-2表达增强，Bcl-2表达降低；主动脉切片发现动脉内膜不连续，部分剥脱，平滑肌细胞排列疏松、紊乱，主动脉壁均见到程度不同的脂质浸润，其病变弥漫，泡沫细胞较多，可见斑块突出管腔；干预组，血浆中同型半胱氨酸含量、血脂水平显著低于对照组和模型组(P＜0.05)，主动脉MCP-1mRNA、VCAM-1、MMP-2表达显著弱于对照组和模型组(P＜0.05)，Bcl-2表达增强(P＜0.05)，主动脉病理形态学和免疫组织化学检测示病变轻于模型组。

Pathological sections were made for haematoxylin and eosin staining and immunohistochemical staining.

制作HE染色和免疫组织化学染色病理切片。

And 4 of experimental group and 2 of control group were killed at 1, 3, 5, 10, 14, 20, 25 and 30 days postinfection, respectively. All the sera, the lymphocyte suspension of peripheral blood, thymus, Fabricius bursa and spleen, were collected.

本研究用AEV-NH937株组织毒1∶100倍稀释，0.03ml／只脑内接种1日龄SPF雏鸡，分别在攻毒后1、3、5、10、14、20、25和30天迫杀实验攻毒组4只，对照观察组2只，分离血清，制备外周血、胸腺、法氏囊和脾脏的淋巴细胞悬液，同时采集脑组织，10％福尔马林固定，制备石蜡切片，做H.E。

Aggressive fibromatosis is a disease of benign ,clone hyperplasia of fibrocyte.β-catenin,which starts downstream gene transcription,was found concentrated in cell nuclei in sporadic patients.

侵袭性纤维瘤病是一种良性克隆性纤维细胞增生疾病，在散发病人的组织病理切片中发现β-catenin向细胞核内浓聚，即向核内转移启动下游基因转录。

All of the tissues were fixed in 10% neutral formalin and enbedded in paraffin.

全部组织经 10％福尔马林固定，常规脱水后石蜡包埋，连续切片。

With the help of Axioplan 2 imaging system, we quantified the change of expression of ICAM-1, MUC5AC, goblet cell and NF-κB. Analyze the results by SAS method. Results (1) Grouped by index of lung function, the expression of ICAM-1 at the level of gene and protein had increased significantly in the epithelial cells of patients with COPD, compared with group of normal lung function.

取病灶远端的正常叶段支气管组织制备石蜡切片，原位杂交检测气道上皮中ICAM-1的mRNA的表达量；免疫组化检测气道上皮中ICAM-1和MUC5AC的蛋白表达变化；AB/PAS特殊染色法检测患者气道上皮细胞中杯状细胞的含量；PCR扩增法检测原代培养的气道上皮中MUC5AC的基因表达量和NF-κB的变化。

Tissue samples were examined on 0, 7, 14, 21, 28 and 35 d with micro section paraffin and histochemistry staining method.

分别于0，7，14，21，28和35d取材，利用显微石蜡切片、组织化学染色的方法检测其肝、消化道和肌肉细胞糖原、酸性磷酸酶和碱性磷酸酶细胞化学特征。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。