组织切片

Figure 2 Staining sections of blood vessels in the composite of BMP group at different time points Immunohistochemistrical staining,×100

图2 复合rm-BMP人工骨组不同时期血管染色切片免疫组织化学染色，×100

Model group and the normal group were taken distilled water,rats in the Tangshukang group were taken Tanggukang powder, western medicine control group were taken Calcium Carbonateand alpha D3. once a day for 12 weeks. Ca,P, BALP,BGP in blood and bone miner density;bone mineral contents, osseous vitodynamics,the content of Ca and P in the cremains were tested respectively.The femur was taken and detected the expression of TGF-β_1imRNA , the duodenum was taken and detected expression of VDR mRNA and CaBp-D9K mRNA with situ hybridization.

分别给予糖疏康、碳酸钙阿法迪三和生理盐水灌胃给药。12周后取材，测体重、血糖、血清钙、磷、血清骨源性碱性磷酸酶、骨T-羧基谷氨酸蛋白、骨密度、骨矿含量、骨的生物力学、骨干重、灰重、骨钙、磷等指标、组织病理切片骨形态计量学指标测定、并进行维生素D受体mRNA、转移生长因子-β_1(TGF-β_1)mRNA、钙结合蛋白(CaBp-D9K)mRNA原位杂交。

Three gastric noncancerous tissues were cryo-sectioned, stained with Cresyl Violet, and pathologically rechecked. Epithelial cells were obtained by LCM and RNA was isolated. Agilent 2100 bioanalyzer was used to check the RNA quality.

选取3个低温保存的胃癌旁组织样本，冰冻切片进行甲酚紫染色和病理学检查，利用激光显微切割技术分离非癌上皮细胞，提取RNA并以Agilent 2100生物分析仪鉴定RNA的纯度和完整性。

Methods Normal adult SD rats were perfused and cut into sections on a cryostat. Immunohistochemistry, immunofluorescent and Nissl staining technique were used.

正常成年SD大鼠5只，常规固定，恒冷箱切片，免疫组织化学、免疫荧光染色、尼氏染色。

To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性，我们利用原位杂交和免疫组化等方法，研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动，结果表明：（1）热对各阶段的雄性生殖细胞都有损害，粗线期的初级精母细胞尤为敏感，实验性隐睾内的精原细胞尚未完全受到"致命"影响，说明精原细胞对热有一定的耐受性，但即使成为粗线期／双线期初级精母细胞，却未能获得由有丝分裂过渡到减数分裂的能力，呈现不同程度的凋亡，所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞，更谈不上长形精子细胞和精子的形成；（2）腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录，说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的；（3）正常兔睾丸组织中，〓在精原细胞和粗线期／双线期精母细胞中均有表达：cyclin B1蛋白在减数分裂前期的粗线期／双线期初级精母细胞中的表达量增加，于变态末期的精子细胞中达高峰。

AIM: There is disadvantage in the conventional method of making bone decalcification and complete sections.

目的：常规的脱钙骨切片制作方法在脱钙、组织结构完整性等方面存在一定的缺陷。

Effects of fixation and decalcification on the immunohistochemical localization of bone matrix proteins in fresh frozen bone sections ［J］.

改良脱钙液制作脱钙骨石蜡切片与传统方法的比较［J］。中国组织工程研究与临床康复，2008，12（11）：

Decalcification is thorough, structure is clear and tissue integrity is better by the modified method of making rat thigh bone and tibia sections.

改良的脱钙大鼠股骨和胫骨石蜡切片制作方法能使标本脱钙更彻底、结构更清晰、组织完整性更好。

Observe the bone formation and depauperation of alveolar bone by X-ray and histological examinations,using those of the extraction sockets by normal healing as control.

于术后2、4、8周分别将犬处死，取拔牙创处牙槽骨标本，行X线和组织学切片检查，观察成骨情况及牙槽骨萎缩情况。

But once the supplemental levels of vitamin E were higher than 400mg/kg no difference in feeding failure was detected, indicating deficiency of vitamin E detrimentally affected fish feeding. Plasma AST activities in the 0 and 3000mg/kg groups were significantly higher than those of the other groups.

维 他命E 添加亦影响肝脏天门冬胺酸转胺酵素活性，添加量最低与最高（0 与3000 mg/kg ）两组显著高於其它三组，组织学切片显示低浓度组肝细胞有空胞化的情形发生。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。