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组织切片

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ObJective To investigate the distribution of hematoporphyrin monomethyl ether in rabbit choroidal melanomas by dye fluorescence angiography and fluorescence microscopy.

目的 应用血管造影和组织冰冻切片荧光显微镜观察的方法,研究国产新型光敏剂血啉甲醚(hernatoporphyrin monomethyl ether,HMME)在兔脉络膜黑色素瘤眼部组织的分布。

This paper presented histopathology of the syndromes between parotitis and putrid skin disease of Soft shelled Turtle in comparison with health individuals.

对鳃腺炎与腐皮病并发症的鳖的肝、脾、肾、肺、肠和精巢等 6种器官进行病理组织学切片,并与健康鳖的相应器官组织进行比较。

AIM: To observe the expression and distribution of C5a receptor(C5aR) on epiretinal membranestissue from patients with proliferative retreous body retina lesion and cultured retinal pigmentary epithelial cells.

目的 :检测手术中取出的增生性玻璃体视网膜病变患者的视网膜前膜组织及培养的人视网膜色素上皮细胞中的C5a受体(C5aR)的表达及分布,阐明C5a受体在发生PVR的病理过程中可能的作用。方法:手术取出ERM组织,常规制备石腊切片。

Day puppies were inoculated by abdominal cavity and nasal cavity with CDV,canine distemper patho-sample of duplicating meet with success and through differentdrug of treatment, specific antibody, dexamethasone, causing death by letting. Theresults of pathohistology observation on 14 PID: Control group, the hearts showedcardiac muscle hemorrhage and necrosis, the livers showed a large area of hepatictissue hyperemia, hemorrhage and necrosis, the spleens showed the quantity oflymphocyte grow downwards, the blood vessels of lung congested, congestion andhaemorrhage were serous fluid exudation, the kidneys showed heavy haemorrhageand hyperemia, the cerebrum showed the number of microglial cells of cerebrumincreased.

通过特异性抗体和地塞米松、特异性抗体、地塞米松三种不同的方法治疗后,放血致死,取材作病理切片,观察各组病理组织学变化,结果为:感染后14d阳性对照组的心脏表现为心肌出血、坏死;肝脏表现为肝组织大面积充血、出血坏死;脾脏表现淋巴细胞数量大量减少;肺脏表现为肺泡结构破坏,有坏死灶;肾脏表现为弥漫性的充血出血;大脑表现为胶质细胞增多。

In this thesis, rat-tails were used as experiment objects, there are 40 slices including transverse and longitudinal, HE-dyed and undyed. Excited by femtosecond laser, the changes of backward SHG and TPEF versus sample preparation, excitation wavelength, excitation power, penetration depth and so on were observed by two-photon laser scanning confocal microscope, effect factors of backward SHG and TPEF and differences of both were discussed and compared, and related comments were also presented.

以鼠尾组织作为实验对象,共40个切片,分为横向和纵向、HE染色和未染色四组,采用飞秒激光器作为激发光,用双光于激光扫描共焦显微镜(two-photon laser scanning confocal microscope, TPLSCM)观察和分析了样品在不同的制备方式、激发波长、激发功率、扫描深度等条件下的背向SHG和TPEF的变化曲线,讨论和比较了生物组织的背向SHG和TPEF的影响因素以及二者之间的异同,并尝试对实验现象做出了一定的解释。

Methods Twenty-two adolescent SD female rats were selected. All rats were killed by dislocation of cervical vertebra, the eyeballs were in paraffin imbedding and made to a series of sections, using immunohistochemical method; ERα and ERβ distribution were investigated in uvea tissue of rats; and quantitied by Tanaka scores analytical method. The uteri of rats was used as positive control and PBS as negative control.

选择青春期SD雌性大鼠22只,采用颈椎脱臼处死大鼠,取眼球作常规石蜡包埋,连续切片, SP免疫组织化学方法显示ERα、ERβ在葡萄膜组织的表达;采用Tanaka记分法对ERα、ERβ进行定量分析,并设正常大鼠子宫作阳性对照;PBS代替一抗作阴性对照。

The control group and exercise groups were killed by decapitation respectively at rest and immediately after exhaustive exercises, whereat 3 milliliters of blood taken from cervix were used as serum preparation.

安静对照组和运动组大鼠分别于安静状态和运动后即刻断头处死,颈部取血3ml,制备血清,取大鼠心肌组织分别制备电镜检测标本、石蜡切片、组织匀浆液和线粒体匀浆液。

INTERVENTIONS: In experimental group, the emetic, cisplatin, was injected abdominally 10 mg/kg. In the control, the physiological saline of same dose was injected. Afterwards, the activity changes in rats were observed at room temperature, quiet and light-avoided environment. Six hours later, the brain tissue was collected for frozen continuous sectioning. Immunohistochemical staining method was used to observe the distribution of Fos positive neurons in brainstem and forebrain nuclei and to count positive cell.

干预:实验组腹腔注射催吐剂顺氯氨铂10 mg/kg,对照组注射等剂量的生理盐水后,于室温、安静、避光环境下观察大鼠的行为学变化。6 h后取大鼠脑组织,进行冷冻连续切片,用免疫组织化学染色方法观察大鼠脑干和前脑核团的Fos阳性神经元的分布,并进行阳性细胞记数。

Histopathologic features demonstrated acanthosis, sclerotic collagen and some interspersed lymphohistiocytic infiltration at the periphery of the lesion.

组织病理显示所有的切片均表现为纤维组织不同程度的增生,其外周血管充血及炎细胞浸润。

Using CP (Colophony-Paraffin) embedding tissue section technique and immunocytochemical method (Streptavidin-Peroxidase, SP), we have investigated the distribution of GABA and 5-HT immunoreactive neurons in the brain of female and male ants, Camponotus Japonicus. Special attention has been paid to the comparative research on the differences between female and male ants. We also try to give possible interpretations leading to these differences in the behavioral aspects.Here we show that in the brain of female and male C.

本实验采用树脂石蜡组织包埋切片技术(国家发明专利ZL98125709.7)和链霉菌抗生物素蛋白-过氧化物酶(Streptavidin-Peroxidase,SP)免疫组织化学方法,对日本弓背蚁雄蚁和雌蚁成虫脑中的GABA和5-HT能神经元的分布进行了详细的研究,比较了雄蚁和雌蚁的异同,并对可能造成这些差异的行为学联系进行了初步的探讨。

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