组织切片

Intestine, lung, heart, kidney and liver tissue samples were also collected after reperfusion 2 hours, and (1) protein concentration of bronchia alveolus lung fluid ,(2)HSP70 expression in heart and kidney (3) histology pathology and (4) oxidation injury (MDA and GSH-PX) in above tissues were determined.

休克2小时，收集小肠、心肌、肺、肝、肾等组织用于测定(1)支气管肺泡灌洗液的蛋白含量(2)免疫组化检测心脏和肾脏HSP70的表达(3)组织病理切片(4)组织抗氧化指标。

Familiar with tissue selection method, tissue section, Hematoxylin Eosin staining, and simple microscopic examination of organs.

熟悉组织取材，包埋，切片，染色等常规病理组织学技术，并能简单分析显微镜下组织结构。

M ? min~(-1). All rats were broken the neck after the anaesthesia on the berry to fetch the liver organization, a part was used to test the organization glycogenic content and NO content, another part to prepar paraffin wax section and to measure hepatocyte apoptotic and the expression of Bcl~2 and Bax protein.

按实验设计，所有大鼠均在实施腹腔麻醉术后断头处死取肝脏组织，一部分肝组织用以测定大鼠肝糖原含量和NO含量，另一部分制备肝组织石蜡切片，用以检测大鼠肝凋亡细胞和Bcl-2、Bax的表达。

On the top of the beauty in histology is the combination of the structure and its function.

从人体细胞形态、切片的色彩、组织结构的形态以及层次比例等方面展示了显微镜下的人体组织结构的形态美，人体结构与功能的和谐构成的生命之美是人体组织学美的顶峰。

The results are as follows:(1) The morphological investigation indicated that the nectaries werelacked on the main nervation, calyx and bract of cotton line 97014 comparedwith TM-1. Alveolate hollow was absent at main nervation of the mature leaf.Results of cytological analysis showed that 97014 lacked nectariferous structure.Where nectary inserted were parenchyma cells, cupped structure and brownnectariferous substance on the lower epidermis were not found.

主要研究结果如下：(1)以棉花无蜜腺品种97014与有蜜腺棉花遗传标准系TM-1为材料，进行形态学比较研究，结果表明棉花无蜜腺品种97014叶片中脉、花萼和苞叶上都缺少蜜腺，成熟叶片叶脉、花萼和苞叶上均无蜂窝状凹陷；组织与细胞切片观察发现，97014缺少蜜腺组织结构，在蜜腺着生处为排列整齐的薄壁细胞，且在下表皮看不到凹陷的组织结构和褐色的蜜腺分泌物。

Rats with CHF were divided randomly into two groups, one group was injected dexamethasone(1mg /kg) intramuscularly at the first and forth day respectively,the other group was injected equi-volume saline likewise, and observing the changes of symptoms of rats with CHF; measuring rats hemodynamics index including blood pressure, heart rate and left ventricular end-diastolic pressure 4 days later. Part 2: To measure every group rats myocardialα1、β1、β2、β3-AR density by immunohistochemical method ,and to understand the AR density changes .Taking the first part rats'myocardium tissues for testing myocardialα1、β1、β2、β3-AR density and 5 normal rats for normo-contrast group and taking gray scale of slice as AR density. Part 3: The study enrolled 35 patients with DCHF. Patients were included if they had orthopnea and refractory edema due to acute DCHF that was severe and had taken more 1 week hospitalization and intravenous therapy in addition to diuretics.

观察心衰大鼠的症状，4天后对两组大鼠进行血流动力学检测，检测指标包括血压、心率和左室舒张末压；第二部分：取第一部分实验动物大鼠的心肌组织，并取5只正常大鼠的心肌组织作为正常对照，用免疫组化法检测大鼠心衰前后及糖皮质激素治疗前后大鼠心肌组织肾上腺素能受体α1、β1、β2、β3受体的变化，取镜下切片的灰度值反应受体的密度；第三部分：研究对象为临床难治性心衰病人35例，病人的选择标准包括由于急性失代偿的心力衰竭出现的端坐呼吸，难治性水肿，患者病情严重，住院超过1周，包括利尿剂之外药物的静脉治疗。

Disk height index of intervertebral disks on X-ray films, areas and average T2 relaxation time of high intensity zone of disks on T2-weight MRI and histological score on pathological sections （HE staining, Masson staining and safranin O-fast green staining） were measured at 2 months, 4 months and 6 months after operation.

术后2、4、6个月时对正常及模型椎间盘观察X线片上的椎间盘高度指数、MRIT2加权相上的椎间盘高信号区的面积和平均T2弛豫时间、椎间盘纤维环损伤区的形态学变化、组织病理切片（HE染色、Masson三色胶原染色和番红O-固绿染色）椎间盘组织形态学变化、生物化学变化和组织学评分等指标。

METHODS Sterile polyvinyl alcohol sponge discs which were labeled as physiological saline group and sinapine groups were implanted into the subcutaneous part of rat.

方法将聚乙烯醇海绵盘随机分为生理盐水对照组和不同剂量芥子碱组，植入小鼠皮下，构建盘状血管生成模型，观察术后12d的血管生成情况及组织生成情况，以蛋白定量检测试剂盒检测在海绵组织中的可溶性蛋白含量，对海绵冰冻切片行苏木精-伊红染色，应用计算机图像处理分析系统分析海绵内组织生长情况。

We found that 1 mutant EG4 cells showed typical characteristics of pluripotent stem cells which had no obvious difference with wild cells; 2 When induced by 10〓 M retinoic acid , mutant EG4 cells differentiated into adipocytes with high frequency compared with mutant cells, suggested that EGFR plays a role in adipocyte differentiation; 3 when injected into nude mice, mutant teratocarcinomas contained a large amount of connective tissues as well as skeletal muscle, while wild EG4 cells produced frequently cartilage, keratinocyte and neuroepithelium.

我们建立了稳定表达胞内区功能缺失的外源EGFR cDNA片段的EG4细胞，分析其生长分化特性，发现 1）突变型细胞可在未分化状态下维持长时间的增殖，表明EGFR对EG多能干细胞表型无明显影响；2）〓 M的维甲酸A(retinoid acid A，RA)诱导后，大部分对照组细胞分化为脂肪细胞，而突变型细胞分化为脂肪细胞的比例明显较少，表明EGFR在脂肪细胞的发育分化中具有一定的调节作用；3）畸胎瘤切片分析显示，突变型瘤组织分布有大量的未分化细胞和结缔组织，分化细胞以肌肉细胞为主；对照组瘤组织含丰富的角质上皮、软骨、神经管等依赖EGFR的分化组织。

All the 45 rabbit-models were sacrificed after treatment and their tissues,including peripherial tumor and central tissue of the ablation area,were sectioned, embedded in paraffin, fixed in 10% Formalinand and spitted constantly with 4μm–section.

切取肿瘤中心组织及边缘组织为检测标本，全部组织经10%Formalin固定，常规石蜡包埋，4μm连续切片，对取材标本行HE染色观察治疗后病理形态学变化。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。