组织切片

And more, ERK contents between group A and B, group C and D were not dramatically different. The pathological lesions indicated that intestinal tissue necrosis was severe in group C and D, which were graded 3 points, but obviously lessened in group A and B, which was graded 1 point, with ITF interfering. Conclusion: Intestinal inflammation was ameliorated after ITF was injected hypodermically or intraperitoneally.

结果：A、B组组织匀浆中ERK的面密度值各为87.68±19.24、82.65±21.35，较C、D组(30.23±7.79、30.74±8.19)明显升高(P＜0.01)，C、D组与E组(5.41±1.46)比较也有升高(P＜0.05)；并且随著ERK的激活伴随著明显的核转位。A、B组间及C、D组间ERK含量无显著差异(P＞0.05)；病理切片显示C、D组HE染色切片见肠壁损伤轻重不一，可见全肠粘膜绒毛坏死，病理评分的中位积分为3分；A、B组肠上皮细胞少量脱落，顶端绒毛坏死，病理评分的中位积分为1分。

Methods Seventy-eight adult female PVGrats were divided into experimental group (n=60) and control group (n=18), intraneural injection technique was used to supply three kinds of NO donors with different half-life(DETA NONOate, DPTANONOate and Spermine NONOate) or saline into the rat sciatic nerve subperineurally under light microscope. At the 1th,2th,3th,7th,and 14th day of duration after sciatic nerve injection, a segment of injected sciatic nerve was taken out and embedded it in TAAB resin after tissue processing. Then it was cut into thin layers (1μm) and the histological changes were observed under high power microscope, meantime the number of degenerated axons were counted.

选择78只雌性PVG大鼠，分为实验组和对照组，以在体神经内微注射实验方法，将三种半衰期不同的NO供体(DETA NONOate，DPTA NONOate和Spermine NONOate)及生理盐水分别注入大鼠左侧坐骨神经的神经束膜下，于注射后的不同时间(1天、2天、3天、7天和14天)取出注射部位的坐骨神经，经组织学处理后做成树脂包埋块，以超薄切片机切片，然后在高倍显微镜下观察并计数神经横断面的变性轴突数目。

The leaf structure and morphology, the structure and localization of oil cells in the leaves of 82 species and 1 subspecies in 10 genera of the Magnoliaceae were comparatively studied by using the methods of tissue clearing, paraffin sectioning and thin sectioning.

首次利用组织透明法、石蜡切片法及薄切片法对国产木兰科10属82种1亚种植物叶片的结构和油细胞的分布密度、结构及其在叶肉中的分布进行了比较研究。

At the end of 12 and 18 weeks, rats were killed, taken the muscle of posterior limb, after separating the gastroenemius、soleus、plantaris, to prepare the frozen section of the each kind of muscle respectively to be confirmed to form the myopathy model by the histochemical stain.(3) RT-PCR、Western blot and immunohisto-chemistry stain were respectively used to detect the expression of NF-κB、MMP-2 and MMP-9 during different period.

2实验组用酒精给大鼠灌胃，对照组用含与酒精体积相等的生理盐水灌胃，两组均喂饲改良的全营养高脂饲料，于12周及18周后，取后肢肌肉，在分离出腓肠肌、比目鱼肌、跖肌后，将各类肌肉均分别地制备成冰冻切片和石蜡切片，经组织化学染色确认成模。

On the frozen sections, the smear preparations, the paraffin sections and the desquamated epithelia samples,various antigens in eight kinds of tissues and cells were demonstrated at the light and electron microscopic levels by using the HRP-SPA immunohistochemical method,and being compared with the PAP method.

应用HRP—SPA免疫组化方法，并对比PAP法，在冰冻切片、细胞悬液涂片、石蜡切片和脱落细胞涂片上，在光镜及电镜水平上观察了八种组织细胞内的不同抗原物质。

Methods We established the model of human hair follicle in vitro in Williams E serum-free medium. We can continuously observe morphologic change of hair follicle under an invert microscope. The length of hair follicle was measured by eyepiece micrometer and DNA synthesis rate of human hair follicle in vitro by measuring the rates of incorporation of 3H-TdR.Histology form was observed by Cryo-section and transmission electron microscope.

在Williams E无血清培养基中，建立离体人头皮毛囊培养模型；通过倒置显微镜观察毛囊的形态变化；目镜测微器测量毛囊长度；3H-TdR 掺入检测毛囊DNA合成率；组织学检测用冰冻切片光镜观察和电镜切片透射电镜观察。

Also observed that the structure of the corresponding organs of the physiological function of physiology, roundworm body wall and the body wall earthworms, roundworm longitudinal serial sections and longitudinal sections form, Once mouthparts in other nematode mouthparts comparison of the education and discussed.

并对观察到的组织的形态结构与其对应的生理器官的生理功能、蛔虫体壁与蚯蚓体壁的比较、蛔虫纵向连续切片与纵向切片形态比较、蛔虫口器于其他线虫口器比较等方面进行了讨论研究。

Objective: To discuss the significance of quality control and standardization of HE and immunohistochemical stainings of puncture liver tissues.

目的：探讨肝脏穿刺组织HE染色切片及免疫组化染色切片的质量控制与标准化操作在提高病理诊断准确率中的意义。

In the positive group of the micrometastasis, the difference was significant statistically ( p 0. 01). There was significant difference of the recurrence rate between the two groups (p .01).ConclusionHie method of conjointing the intermission - continuous - section and CK20 immunohistochemistry technique may be valuable to re-estimate the LN metastasis status and stage of colorectal cancer and predict the prognosis.

1、间断切片法结合CK20免疫组织化学方法有助于检出Dukes B期大肠癌淋巴结微转移，增加每个淋巴结的切片数量有助于提高微转移的检出率； 2、Dukes B期大肠癌低分化及癌肿侵犯肠周径＞3／4者易发生淋巴结微转移； 3、淋巴结微转移阳性组与阴性组的生存率与复发率有显著差异

30 Dogs and 180 rats were divided into 6 groups randomly and respectively,then irradiated by pulse microwave with 5 different power densities ranged from 1.004 mW/cm~2 to 0.974 kW/cm~2 in different group.12 months after irradiation,animal temperature,heart rate,electrocardiogram and serum myocardial enzyme spectrum were tested to study the changes of heart function,and the pathologic histological changes of myocardium were observed with paraffin slice dyed by HE,Pollak and PTAH respectively,and ultrastructu...

30只犬，180只大鼠，各随机分为6组，分别以5个功率密度(从1.004 mW/cm2至0.974 kW/cm2)脉冲微波辐照，在辐照后即刻(0 h)至12个月的不同时相点，分别检测其体温、心率、心电图、心肌酶谱等指标，以研究心脏功能的变化；分别取左右房室肌、窦房结、血管等作石蜡切片，并HE、Pollak、PTAH染色，以研究其病理组织学变化；取大鼠心室肌作超薄切片，电镜观测其超微结构的改变。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。