组织切片

Five days following hormone additions, we obtained transverse sections of mid-stems of A. anomala and S. variegata and analyzed aerenchyma formation using E80i Nikon microscope, ACT-2U and Simple PCI software.

处理5 d后，采用切片法制备其茎中部横切面切片，用E80i Nikon显微镜进行观察，并运用ACT-2U和Simple PCI软件分析野古草和秋华柳茎中通气组织的形成情况。

Methods: We divide the rats into 5 groups in random, with Morris method, detect the change of some active oxygen radicals such as super-oxide demitasse, Nitric Oxide Synthase, malondialdehyde etc. Also, we detect the change of cholinesterase, Monoamine Oxidize; using HE,PAS staining, immuno-histochemical technique, detect hippocampus CA1 cerebral pathology slices optical density value of NOS, CHE and lipofuscin of neural cell; detecting apoptosis rate and the expression of P53 , which are neuronal apoptosis related genes.

大鼠随机分为5组，采用"Morris水迷宫"法观察脑缺血性模型大鼠学习记忆功能，检测超氧化物歧化酶、一氧化氮合酶、丙二醛等自由基代谢异常的改变，检测胆碱酯酶、单胺氧化酶等老化相关酶的变化、脑组织神经细胞免疫组织化学技术、检查海马CA1区脑组织的病理切片，检测NOS、CHE光密度值；流式细胞技术：检测细胞凋亡率，及促细胞凋亡基因、P_(53)蛋白表达；病理切片采用HE染色、PAS染色及免疫组化法检查，检测对大鼠海马神经元细胞一般病理变化及脑细胞内脂褐含量。

Objective To aid at the histological evaluation of hyaline cartilage and bone, we provide a simple and rapid method of tissue preparation for articular joint histology. Methods A defect 3 mm in diameter and 4 mm in depth, penetrating the subchondral bone plate, was created on the medial femoral condyle of rabbit knee using a stainless-steel electric drill.

目的：探索一种简单、快速的制作兔膝关节骨组织石蜡切片的方法，观察关节软骨和骨组织微观结构，为制备高质量骨组织石蜡切片和组织病理学研究提供实验基础。

A plantlet had been obtained. Histological sections proved that the embryos had a typical histological structure of monocot somatic embryos.

组织学切片证明所诱导的愈伤组织是胚性组织，其所产生的体胚具有典型的单子叶植物体细胞胚的组织结构。

There were inflammatory cells infiltrating in the myocardium sparely and no calcification in the pericardium in IVIG and IVIG+VitC groups. The size of spleens enlarged especially in IVIG and IVIG+VitC groups.

心脏分为三部分：一部分作病理切片和H-E染色，一部分作冰冻切片，在荧光显微镜下用直接免疫荧光法测定心肌组织中沉积的IgG水平，一部分作电镜检查。

Objective To compare the two diagnostic methods（bone marrow fragments smear and bone biop-sy）in hematology diseases.Methods Take bone marrow using routine aspiration and make bone marrow fragments smears;using trephine take a piece of bone marrow send it to the pathology department have a histodiagnosis.

目的 对造血系统疾病进行骨髓小粒拉片与切片两骨髓检查的方法进行比较方法常规髂后上嵴骨髓穿刺抽取骨髓液制成涂片；取骨髓小粒制成拉片；再用环钻法取骨髓组织制成切片并作相应的染色，结合临床进行分析。

And 21 d after 0.4 mm occlusal alteration treatment, mechanical pain thresholds of bilateral masseter muscles were quantitatively measured by modified electronic anesthesiometer in control group and occlusal interference group. The rats were euthanized by transcardiac perfusion after deep anesthetization at different time points.

实验组动物在各时间点测试后于全麻下行心脏灌注，对照组在21 d测试完毕后全麻下行心脏灌注，取双侧咬肌组织，制备石蜡切片，对切片进行常规HE染色、抗PGP9.5和抗SP免疫组化染色。

Objective In order to reduce the time which the patient stayed on the operative table, it must be to short the time to make the section, to rise the quality to make the section, so as to rise the rate of diagnose exactly. Methods The unfixed tissue blocks were put into the freezing chamber and cut it for 5 or 10 μm section at once after it were frozen,then put it into the fixactives for 20 seconds and stained with haematoxylin and eosin.

目的 了减少患者在手术台上的停留时间，必须缩短切片制作时间，提高制片的质量，如此可提高诊断准确率方法将未经固定的组织块放入冷冻箱内，冷冻后切片5μm或10μm，即刻于固定液里固定20s，用HE染色。

A firm tumor was identified adjoining the capsule of the superficial lobe of the parotid gland. Both frozen section and pathologic reports showed histiocytic necrotizing lymphadenitis.

术中发现紧邻腮腺浅叶被膜有一坚实、大小3×3cm的肿瘤，冰冻切片及永久切片报告均为组织球性坏死性淋巴结炎。

ABSTRACT Objective To discuss the clinical pathologic features and differential diagnosis of mixed germ cell sex cord stromal tumorwith malignant variant of germ cell tumor Methods The clinical pathologic datum and immunophenotype were studied in the case of ovary MGCSCST with malignant variance of germ cell tumor Results The patient's genital anatomy and female phenotype were normal,karotypes was 46xx with uterogestation The tumor tissue consisted of mixed germ cells and sex cord stromal cells with evident malignancy of mixed germ cells There was no atypical structure of gonadoblastoma in tumor tissue Conclusion MGCSCSST has complex morphosis,thus,to prevent misdiagnosis,more samples should be collected and more slices be cut The patient may have prognosis malo when there are high malignant variants of mixed germ cells in tumor tissue

目的 探讨混合性生殖细胞性索间质肿瘤(mixed germ cell sex cord stromal tumor，MGCSCST)伴恶性生殖细胞肿瘤变异型的临床病理特点及鉴别诊断。方法卵巢MGCSCST伴恶性生殖细胞肿瘤变异型的病例进行临床病理特征和免疫表型分析。结果患者生殖器的解剖结构和女性表型正常，染色体组型是46xx，足月妊娠。肿瘤组织由混合性生殖细胞和性索间质肿瘤构成，混合性生殖细胞有显著的恶性特征。瘤组织中没有典型的性腺母细胞瘤结构。结论 MGCSCST有复杂的形态结构，因此更多的取材和切片可以防止误诊，当肿瘤组织中的混合性生殖细胞有高度恶性变异时，患者预后不良。卵巢肿瘤；生殖细胞性索间质肿瘤；免疫组织化学

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。