组织切片

For further studying the genetic mechanism of the reproductive isolation phenomenon of interspecific hybrid, paraffin sections of testes from 13 sexually mature male mules (Equus asinus×Equus caballus) and 12 hinnies (Equus caballus×Equus asinus) were prepared, and the samples of Giemsa meiosis and AgNO 3 synaptonemal complexes were observed and compared with spermatocyte meiosis of horse and donkey under light microscope and electronic microscope.

为了进一步探讨种间杂种生殖隔离现象的遗传机理，本研究采用性成熟公骡的睾丸为材料，制备组织切片和减数分裂联会复合体标本进行观察，发现个别性腺发育较好的个体虽然减数分裂存在大量异常，但确有少数精母细胞完成了减数分裂并产生精子。

The degree of the plumping of the cattle hide and the removal of the hair were evaluated during the li- ming process;the protein,suspending substance,sodium sulfide and effective calcium oxide contents in the liming liquor and the fat content in the limed hide were measured;also,the tissue slice of the limed hide was observed by optic microscope.

将不同量的十二烷基硫酸酯钠、十二烷基三甲基氯化铵和平平加O分别添加到浸灰液中，观察浸灰过程中皮膨胀情况和毛脱除情况，测定浸灰废液中蛋白质、悬浮物、硫化钠和有效氧化钙含量及浸灰皮油脂含量，并对浸灰皮组织切片进行显微镜观察。

At the culmination of the experiments, the brains of mice were sectioned and scrutinized through immunostaining.

试验最后阶段，通过免疫染色细致观察小鼠大脑的组织切片。

Made KAP6- 1.2 cRNA probe, In situ hybridization with the adult and fetal skins of arbas cashmere goat.

制备KAP6-1.2 cRNA探针，通过组织切片原位杂交在皮肤中进行定位表达分析。

Observation embryonic skin paraffin section of the Inner Mongolian Arbas cashmere goat by light microscope.

制作内蒙古阿尔巴斯绒山羊胚胎皮肤组织切片，显微镜下观察照相。

Tissues from condyles of femur were observed by HE staining and 2, 4, 6-trinitrophenol rosein staining, and mineral apposition rate of bone was calculated.

术后18周时，行股骨髁组织切片HE染色和品红苦味酸染色，观察植入物降解情况并比较骨矿物质沉积率。

BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

实验方法1分离、纯化、培养、鉴定兔BMSCs并以SPIO以25μg Fe/ml培养液浓度标记，对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察。2将不同细胞浓度标记细胞管(1×104/ml、5×104/ml、1×105/ml、5×105/ml、1×106/ml、5×106/ml)，以不同扫描序列T1WI，T2WI，T2*WI(GRE进行MR成像，再选择相同细胞浓度组(5×104/ml、1×105/ml)进行不同时相MR成像，并测量信号强度，进行统计学分析。3缺血再灌注肾损伤模型建立和处理，然后将标记和未标记细胞(5×105个)经耳缘静脉移植入家兔体内(共16只：注入标记细胞者10只，注入未标记细胞者6只)，两组均于注射前、注射后第0、1、3、5、8天应用MRI对移植细胞进行活体示踪并与肾脏组织切片对照，然后对收集的信号强度进行统计学分析。

Gradients of K(superscript +), Na(superscript +) and protein along midgut were determined. The permeability of midgut segments to K(superscript +), Na(superscript +), Ca(superscript 2+), Mg(superscript 2+) and methyl blue was observed.

对中肠细胞进行了组织切片观察；测定K，Na和蛋白质沿中肠的浓度梯度，并观察了中肠对K，Na，Ca(上标 2+)，Mg(上标 2+)和甲基蓝的通透性。

Results The histological observation of the cerebellar tissue in the animal experiment revealed that embryonic grafts could survive and that mitoses of the host Purkinje cells of the implanted group were increased substantially over the control groups. The short-term therapeutic effects were good in the 6 patients following cerebellar transplantation.

结果 动物实验的组织切片证实移植物在受体脑内再生，并且可见浦氏细胞分化成熟和迁徙到颗粒细胞层。6例患者接受小脑移植后近期疗效良好，症状和体征及客观指标均有改善。

The contents of serum fluorine were determined, thyroid tissue slices were made , the weight of thyroids was weighed ,and the clinical manifestations of experimental chicks were observed.

每30 d采集1次样品，测定血清中氟含量，制作甲状腺组织切片，称量甲状腺重量，观察鸡的临床表现。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。