组织切片

Objective To explore a new method of cochlear sectioning to get high qualitative cochlear section catering for immunohistochemistry of cochlea.

目的 探索一种新的耳蜗组织切片方法，以获得高质量的切片，满足其免疫组织化学染色的需要。

The liver fibrotic model was induced by CCl4 i.p for 5wk. Telmisartan was given at the same time model establishment in each intervened group by administration lasted for 6wk. Decollate to get the blood of all rats after 7 weeks . ALT、AST and ALB of serum was detected by automatic biochemistry equipment. Hyaluronic acid was detected by ELISA detection. Liver and spleen was obtained to calculate the index. The liver pathological changes were observed in each group of rats after HE staining to evaluate the therapeutic effect of Telmisartan; TGF-β1、PPAR-γand PDGF of liver tissue was detected by the method of immunohistochemistry respectively; The mRNA expression of TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γand iNOS was detected by RT-PCR detection, respectively.

腹腔注射CCl4制备SD大鼠肝纤维化动物模型，连续灌胃6周，处死动物取血，检测血清ALT、AST、ALB；ELISA法测定血清透明质酸；取肝、睥，并计算肝、脾指数，组织切片HE染色后进行组织病理学疗效评估；用SP免疫组化法测定肝组织TGF-β1、PPAR-γ及PDGF蛋白表达；半定量RT-PCR方法检测肝组织TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γ和iNOSmRNA的表达。

Methods EOM specimens were obtained from 12 patients with severe TAO and 10 healthy subjects within 1 hour after accidental death and the paraffin-embedded sections were observed by streptavidin-peroxidase immunohistochemical methods.

方法收集手术治疗的12例严重的TAO患者的眼外肌组织标本及10例健康人意外死亡1 h内取出的眼外肌组织标本，4％中性甲醛固定24 h，石蜡包埋组织，作连续切片，采用免疫组织化学链霉卵白素·过氧化物酶连接法对12例严重TAO患者及10例健康人的眼外肌石蜡组织切片进行光学显微镜观察。

Methods The cochlear samples cochlea of guinea pig and mouse were infiltrated with increasing concentrations of sucrose, followed by degassed embedding medium before final orientation and freezing. The sections were stained by hematein、eosin and immunohistochemistry. The morphology of the tissue sections was observed.

取1天龄大鼠、成年豚鼠、成年小鼠耳蜗标本经蔗糖快速浸透，用离心管定向并用新型组织包埋剂Tissue-Tek包埋，液氮中快速冷冻后在恒冷箱切片机中切片，然后分别做苏木素、伊红染色和免疫组化染色，观察组织切片的形态结构。

Fabrication of tissue section:after spinal cord injury ld,3d,5d,8d,14d,21d,we sacrificed rats in batch.4% formaldehydum polymerisatum were pured into the cardiac muscle and fixed for 24h.Then obtain the specimen of spinal cord from T_5 to T_(13).Establishing slices,HE dyeing,bcl-2 dectation,terminal doxynucleotidyl transferase-mediated dUTP nick end labeling.On the fourteenth day after spinal cord injury,we detect Gale grading score and tiltboard experiment.

组织切片的制备：脊髓损伤后1d，3d，5d，8d，14d，21d共6个时间段分批处死动物。4%多聚甲醛心肌灌注固定24h，切取每只大鼠T_5～T_(13)节段脊髓组织，常规制成切片，给于HE染色，Bcl-2检测，TUNEL原位末端标记以及伤后第14天检测Gale评分和斜板实验，检测大鼠脊髓损伤后细胞凋亡和脊髓神经功能恢复情况。

Provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation in tissue sections and fixed cell preps.

提供完整的组份，包括阳性，阴性对照组织切片，便于检测组织切片和固定细胞中的片段化DNA，结果可通过显微镜分析。

Historically, critical technical advances made during the mid and late 19th century, including the development of the first high-quality microscopes, the manufacture of synthetic stains, the discovery of effective tissue fixation and embedding techniques, and the development of modern-style microtomes for cutting thin tissue sections produced the first revolutionary shifts in brain tumor classification.

历史上，在19世纪中期及末期出现的重大技术进步，包括第一台高质量显微镜的发展，人造染色剂的制造，有效的组织固定和包埋技术的发明，能够切割较薄组织切片的现代设计切片机的发展等，促成了第一次脑肿瘤分类的革命性变化。

Detecting each marker and cmparison the location of sequential tissuesection could determine the area loceation relationship of the markers, but it is difficulte to determine their correlation intra-cells exactly. No report of detecting virus genome and antigenes on a one section with triple staning techniques exists in the lit erature. in this report. by combining immunohistochemistry and in situ hybridization (with dioxigenin labelled HBV DNA probe), a triple staining technigce was established. With this technic, on one formalin-fixed and paraffin embeded section, HBV DNA, HBsAg and HBcAg were determinated one after the other.

各个细胞内病毒的复制和表达水平以及与肝细胞病变的关系，是乙型肝炎发病机理研究的一个重要方面：目前常用的在系列切片上对各标志物分别检测，比较观察的方法，一般只能大致了解各待检物在某一区域的定位关系，很难准确地了解它们在同一细胞内的关系：国内外尚未见有在同一张切片上三重标记染色检测病毒核酸及其多种抗原的报道：本文结合免疫组织化学ABC法及地高辛配基标记HBV DNA探针原位杂交，建立三重标记染色技术：在一张福尔马林固定石蜡包埋肝组织切片上先后检测，同时显示HBV DNA、HBcAg和HBsAg。

For the 23 core biopsies suggesting phyllodes tumor, excisional biopsy confirmed the diagnosis in 19 but revealed fibroadenoma in four patients, for a positive predictive value of 83%. Final pathological analysis of the equivocal core biopsies diagnosed fibroadenoma in five patients and phyllodes tumor in four patients. Final analysis determined that none of the lesions were malignant.

23个核心组织切片检查建议为叶状瘤的患者，进行切除的组织切片检查后，确认了19名患者的检验结果，但是有4人为纤维性瘤，因此得到83%的阳性预测值，有歧异的粗针检查进行最后的病理分析后，发现五名患者为纤维性瘤，而四名患者为叶状瘤，最后的分析测定这些肿瘤都不是恶性的。

The results showed that (1) COX-2 mRNA and protein existed in the mature male rat testis. COX-2 was localized in spermatocytes by immunohistochemistry.(2) COX-2 also existed in adult man testis. COX-2 protein was positively stained in spermatocytes and Leydig cells.(3) 2 weeks after administration of rofecoxib, the level of testosterone in the whole testis reached its lower values, being only 50% of control values. However, testosterone level recovered during 4 weeks. After such treatment, histologic examination of these testes showed atrophy of the seminiferous tubules and maturation arrest of spermatogenesis.(4) 2 weeks post-EDS, expression of COX-2 decreased significantly (P.005), in comparison with vehicle-treatment control. 4 weeks after treatment, a new generation of fetal like Leydig cells repopulated in the testicular interstitium resulting in COX-2 expression partially recovered. Although the expression of COX-2 mRNA and protein are enhanced at 8th week after using external testosterone, it wasn't significant higher than control group.

实验研究证明：(1)正常成年雄性大鼠睾丸组织中COX-2在mRNA和蛋白质水平均存在表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞；正常成年男性睾丸组织中同样存在COX-2表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞和Leydig细胞；(2)服用特异性COX-2酶抑制剂rofecoxib 2周后，实验组大鼠睾丸组织内睾酮的含量减少，为正常对照组的50％；持续用药4周后睾丸组织内睾酮浓度逐渐恢复至正常水平；COX-2酶活性降低后病理组织切片显示睾丸内曲细精管萎缩，生精紊乱，持续用药4周时影响最明显；(3)注射特异性Leydig细胞杀灭剂EDS 2周后，实验组大鼠睾丸组织内睾酮浓度降至极低水平时，COX-2的蛋白和基因表达水平也显著低于正常空白对照组（P.005）；使用EDS后第4周，睾丸组织中的睾酮浓度逐渐回升，同样组织中COX-2蛋白表达和mRNA表达水平也相应提高接近正常水平；使用EDS后4周开始给予外源性睾酮，6周和8周时COX-2表达水平的绝对值虽有提高，但与正常大鼠空白对照组比较并无显著性差异，说明外源性雄激素刺激睾丸合成COX-2的作用并不明显。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。