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Under new situations,they appear some flew characteristics in the organized crimes:the number is increasing with a rapid growth;the character is developing more typical towards underworld;the equipment used in the crimes is becoming moderner;the degree of systematization is turning higher and higher.The tendency towards enterprise is changing dearer and clearer;the means is becoming softer and softer;they appear territorial and international tendency.

在新形势下,有组织犯罪呈现出一些新特点:带黑社会性质的犯罪数量上迅速增长,质量上正向典型的黑社会犯罪演化;装备更趋现代化;犯罪的&组织化&程度越来越高,&企业化&趋势更加明显;犯罪手段上越来越重视&软&手段的运用;犯罪活动呈现跨区域化、国际化的发展趋势。

Autosomal dominant polycystic kidney disease , one of the most common monogenetic human disorders, is characterized by the formation and progressive expansion of multiple cystic lesions, which compromise the function of normal renal parenchyma and lead to nearly 10% of cases of end-stage renal failure in adults.

中文题名角质细胞生长因子在ADPKD肾囊肿组织中的表达及对囊肿衬里上皮细胞增殖作用的研究副题名外文题名 Expression of keratinocyte growth factor in ADPKD cystic tissue and effect of proliferation on ADPKD cyst-lining epitheliar cells 论文作者刘沙勤导师梅长林朱有华教授学科专业内科学研究领域\研究方向学位级别博士学位授予单位第二军医大学学位授予日期2002 论文页码总数104页关键词角质细胞生长因子肾病囊肿衬里上皮细胞株肾囊肿馆藏号BSLW /2003 /R692 /1 ADPKD是人类为之付出昂贵代价的一种全身性遗传性疾病,是引起慢性肾功能衰竭的第四位病因,在临床上以肾脏满布大小不一并不断增大的囊性液泡、最终引起肾功能进行性损害为特征性的改变。

Results (1) the laminin synthesis was significantly improved by PBMC from nontreatment group (P<0.05);(2) the 3 H-prolin incorporation was significantl decrease in the GEC treated by PBMC supernatant from nontreatment group(P<0.05);(3) the supernatant of PBMC from nontreatment group stimulated the collagen Ⅲ synthesis(P<0.05), while, there was no significant difference of collagen Ⅳ synthesis between 4 groups;(4) the activity of PBMC supernatant have no close relation with urinary protein excretion, pathological type and clinical type.

结果 (1)肾病极期未经激素治疗组PBMC上清明显促进了GEC合成层粘连蛋白;(2)未治PBMC上清抑制了GEC合成总胶原;(3)未治组PBMC上清促进了Ⅲ型前胶原的合成,而对Ⅳ型胶原的合成无明显影响;(4)肾病患儿PBMC的上述作用与是否足量激素治疗有关,而与尿蛋白能否阴转、肾组织病理类型、肾病临床类型等无直线相关关系。

On the basis of analyzing its history and existing circumstances, firstly, the strategy of pluricellular management is put forward. Secondly, the first bureau"s operation mechanism which focuses on the method of project construction and the problem that the bureau adjuster its enterprise structure and strategy and allocate resources on the basics of the existing strategic capability, facing on rapid changing environment. Thirdly, the view of enterprise"s foreign development, which is not a selection but a reality, is to be held.

在对中国路桥集团公路一局进行历史和现状分析的基础上,在第一个层面,首先提出了立足公路建设市场进行多元化的经营基本战略;在第二个层面分析了公路一局以项目施工法为核心建立向智力型施工企业发展的运行机制,解决了公路一局面对急剧变化的环境,在现有战略能力的基础上,进行组织结构调整和战略资源配置的问题;在第三个层面上分析了企业面对全球化,走出去不是一种选择,而是一种现实,提出了公路一局进行跨国经营的战略,重点论述了通过国际合作参与国际竞争,逐步走向国际市场的竞争手段。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物;培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感,据此可在传代过程中将二者分离纯化,获得纯细胞系;混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞;再加0.15%胰蛋白酶-0.02鞹A 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞,经过2~3 代,即可得到纯化的乳腺上皮细胞系。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物;培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感,据此可在传代过程中将二者分离纯化,获得纯细胞系;混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞;再加0.15%胰蛋白酶-0.02%EDTA 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞,经过2~3 代,即可得到纯化的乳腺上皮细胞系。

Methods 11 cases with asymmetry of the submammary fold were treated with the semi-periareolar insion, and separated the retromammary space, and then cutted up-part of the breast tissue according to the value of asymmetry and fixed upward, and incised part of the skin also(9 cases were done mama augmentation simultaneously).

对11例乳房下皱襞不对称患者,采用乳晕上缘半环形切口,游离乳房后间隙,根据双侧不对称的差值,将过低侧乳房的上极腺体组织弧形切除并向上与胸肌筋膜缝合固定(其中9例同期行隆乳术),切口上缘皮肤作适当切除。

They are difficult to eradicate, even though they are composed of histologically appearing squamoid and columnar epithelium lining cystic spaces filled with oily fluid.

尽管在组织学上由鳞状上皮和柱状上皮构成囊腔,囊内充满脂性液体,但难以根除。

The two can cause ultrastructural pathogenesis widely in many organs and tissues such as hepar, pancreas, lien, ren, pulmo, cerebral, cerebellum, cor, muscular and so on, of them ,caused by i~olates YG97 and Y98 more obvious in epithelial cells of digestive tract and unobvious in epithelial cells of respiratory tract ,but ultrastructural pathogenesis caused by NDV strain F48E8 ,more obvious and more serious in glandularis and muscularis ventriaculi and in epithelial cells of respiratory tract .

将鹅源分离株YG_(97)、鸡源分离株Y_(98)和NDV强毒株F_(48)E_8感染SPF鸡,运用扫描电镜和透射电镜观察该二株病毒对机体细胞的影响,结果显示三株禽副粘病毒均引起机体肝、胰、脾、肾、肺、小脑、大脑、心和肌肉等实质器官组织细胞的超微病变,其中YG_(97)和Y_(98)引起消化道粘膜上皮细胞发生微绒毛脱落、纤毛脱落、分泌颗粒增多、细胞游离面破溃等显著的超微病变,引起气管粘膜上皮细胞的不显著超微病变;F_(48)E_8强毒株引起胃粘膜显著而严重的超微病变;还引起气管粘膜上皮严重的超微病变。

In this essay, the culture methods of corneal epithelial cell and keratocytes have been studied. The method of rabbit corneal epithelial cells has been improved in order to solve the problem that the cells moved slowly and could not form monolayer when the traditional tissue nubbles cultivation was adopted.

本文对兔角膜上皮细胞和基质细胞的培养方法进行了研究,解决了传统角膜上皮细胞组织块贴壁培养时细胞迁出慢,不易形成单层的难题,建立了兔角膜上皮细胞原代培养的方法。

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