组织

We found that 1 mutant EG4 cells showed typical characteristics of pluripotent stem cells which had no obvious difference with wild cells; 2 When induced by 10〓 M retinoic acid , mutant EG4 cells differentiated into adipocytes with high frequency compared with mutant cells, suggested that EGFR plays a role in adipocyte differentiation; 3 when injected into nude mice, mutant teratocarcinomas contained a large amount of connective tissues as well as skeletal muscle, while wild EG4 cells produced frequently cartilage, keratinocyte and neuroepithelium.

我们建立了稳定表达胞内区功能缺失的外源EGFR cDNA片段的EG4细胞，分析其生长分化特性，发现 1）突变型细胞可在未分化状态下维持长时间的增殖，表明EGFR对EG多能干细胞表型无明显影响；2）〓 M的维甲酸A(retinoid acid A，RA)诱导后，大部分对照组细胞分化为脂肪细胞，而突变型细胞分化为脂肪细胞的比例明显较少，表明EGFR在脂肪细胞的发育分化中具有一定的调节作用；3）畸胎瘤切片分析显示，突变型瘤组织分布有大量的未分化细胞和结缔组织，分化细胞以肌肉细胞为主；对照组瘤组织含丰富的角质上皮、软骨、神经管等依赖EGFR的分化组织。

Three hours OLV does not cause injury of the nondependent lung, yet six hours OLV leads to injury of the nondependent lung. Low-dose ALM would not deteriorate the injury of the nondependent lung.

OLV 3hr不会造成无通气肺组织的损伤，而OLV 6hr则引起无通气肺组织的损伤：小剂量ALM在提高HPV反应，改善OLV期间动脉氧合的同时，并不加重无通气肺组织的损伤。

Malondialdehyde production and total superoxide dismutase activity in samples of nondependent lung and dependent lung were detected and analysed in both groups on time of OLV 3hr and OLV 6hr to determine whether long-term OLV led to injury of the nondependent lung and whether the injury would be deteriorated by low-dose ALM.

通过对OLV 3hr及OLV 6hr时两组实验犬无通气肺与通气肺组织标本中丙二醛含量及总超氧化物歧化酶活力的测定，观察长时间OLV是否引起无通气肺组织损伤，并同时探讨小剂量ALM对无通气肺组织损伤的影响。

Methods Malignant tumor tissues were obtained from 11 patients with colon cancer, 15 patients with cervical cancer, 10 patients with breast cancer, 9 patients with esophageal cancer, 12 patients with lung cancer and 16 patients with gastric carcinoma. Nonmalignant tissue was used as negative control. Three tumor cell lines were also studied. Hfgl 2 was examined both at the level of mRNA and protein by immunohistochemistry as well as RT-PCR.

采用免疫组化方法检测了11例结肠癌、15例宫颈癌、10例乳腺癌、9例食管癌、12例肺癌、16例胃癌患者的病理组织切片中hfgl 2及纤维蛋白的表达，分析二者的组织学关系，用RT-PCR方法检测了部分肿瘤组织与肿瘤细胞系中的hfgl 2 mRNA。

Learning organizations were preached in the book of the fifth enforce, which was written by PeterSenge. who is an American management artiste. He resembled numberous experices of the first class of entrepreneurs in America and in the world. And then he wrote the book.In the book he preseroted this kind of orgnization structure.

学习型组织是美国管理大师彼得·圣吉汇集无数美国及世界一流企业家的实践，研究分析世界一流企业组织结构和管理经验，在《第五项修炼》中所倡导的一种组织类型。

To dissect single cells promising to culture from tissues, we researched the time of hydrolyzation of the tissues by tryposin, the result showed that the optimum time of hydrolyzation of the tissues was 5~10min when pretreated with 0.25% tryposin under the optimum conditions of pH8.0 at 37 C.We cultured four tissues: embryo at mediat-anaphase, head of high-age nymphae, spermary and ovary of adult.

组织块分离出来后，为了能获得单个的、有培养前景的细胞，本实验又研究了低浓度的胰蛋白酶在其最适温度和最适pH条件下，获得分离程度适宜的组织块的不同酶解时间，结果0.25％的胰蛋白酶在37℃、pH8.0的条件下酶解组织块的最适时间是5～10min。

Methods BMSCs isolated from rabbit iliac bone marrow were culture-expanded in vitro. The injectable tissue-engineered bone constructed from autologous PRP, FG, and BMSCs was cultured in vitro, and its biological characteristics were observed including the time of gel formation, histological features, seed cell survival and microscopic structures. Results The constructed injectable tissue-engineered bone began gel formation within 20 to 30 s, and after a week-long culture, the gelatine began to degrade, and numerous well viable fusiform cells could be seen to adhere to the bottom of the Petil dish, Scanning electron microscopy identified globular and olivary cells embedded in the fibrin glue, and numerous small particles could be seen around of the cells.

从兔髂骨处抽取骨髓体外培养BMSCs并诱导向成骨细胞分化，抽取兔自体动脉血提取PRP，以FG、BMSCs、PRP共同构建可注射型组织下程骨并体外培养观察其生物学特性如凝胶形成时间，组织学特点、细胞存活情况及其超微结构特征等结果构建的可注射型组织工程骨可在短时间内形成凝胶，体外培养1周时其中细胞生长良好，电镜下见纤维蛋白网格结构致密，种子细胞及血小板颗粒分布广泛。

PR content of the mixed type was higher than that of the hyperplastic t ype; and the PR content of the hyperplastic type was higher than that of the atr ophic type. The relationship between ER and PR contents of the vulvar dystrophy and adjacent normal tissue was in positive correlation. The PR content of vulvar squamous cell carcinoma went down along with the drop of its dedifferentiation degree. The PR content of carcinoma was much lower than that of the adjacent nor mal tissue.

结果：ER的含量混合型高于萎缩型，PR的含量混合型高于增生型，增生型高于萎缩型（ P值均＜0.05）；外阴营养不良及其邻近正常组织的ER与PR的含量之间呈正相关关系（P ＜0 。01），外阴鳞状细胞癌PR的含量随分化程度的降低而逐渐降低，且癌组织PR含量明显低于其邻近的正常组织。

METHODS: A total of 50 white rabbits were randomly divided into anterior cruciate ligament transection group, medial collateral ligament transection + meniscectomy group and normal control group. The specimens were harvested at weeks 1, 2, 3, 4, and 5 after operation. The osteocartilaginous section of the medial femur condyles were observed by gross observation, histological examination, and Markin scores. The contents of proteoglycan and collagen fiber were also measured.

取大耳白兔50只，随机分为3组前交叉韧带切除组、内侧副韧带+半月板切除组分别切除右后肢前交叉韧带、右后肢内侧副韧带和半月板，正常对照组不干预，分别在建模1，2，3，4和5周后分批于膝股骨内髁软骨取材，进行软骨组织大体和组织学观察、软骨损伤程度Markin评分；测定软骨组织蛋白多糖及软骨基质中胶原纤维含量。

Methods: The immunohistochemistry was used to investigate the protein expression of p27 in 46 cases of osteosarcoma and 16 cases of osteochondroma. The expression of p27 and histological category and differentiation levels of osteosarcoma were observed.

应用S-P免疫组织化学方法检测46例骨肉瘤和16例骨软骨瘤组织中p27的表达，并观察p27表达在骨肉瘤组织学分型、分化程度等临床指标之间的差异。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。