组织

Fibroplastic proliferation was observed at 14th days and later.

肝脏碘油栓塞HIFU损伤后即刻损伤组织表现为肝细胞结构破坏，肝细胞核固缩。3天后坏死区与正常组织分界清楚，坏死区细胞完全破坏。14天出现明显增生的纤维结缔组织，包绕坏死组织。

PartⅡ: Analysis of the paraffin embeded tissue slides [obtained from trail and control group at pre-treatment (d0) and post-operation (d15±1 days) ] and frozen section slides (from 4 typicalcases: 1 of IAC group, the other sBRM group at d5, d7 and d10), by H-E stain, monoclonal antibodies (CD3/CD4/CD8/CD68/CD57) marking, SP-DAP or APAAP-AEC stain, and TUNEL in situ apoptotic cell DNA fragments fluorescin labeling.

二。组织病理学研究：对上述两组病人治疗前后原发灶肿瘤组织蜡块分别作H-E和CD3、CD68表型免疫组化染色；并对其中四例典型病人的疗中间断连续活检新鲜组织作H-E染色，CD3、CD4、CD8和CD57表型免疫组化染色和凋亡细胞DNA断片TUNEL法荧光标记检测。

To obtain precise result, itis necessary, in the aspect of hardware, to set up one stable optics system with highSNR, only if so, can we do the measurement with the steady NIR. To do so, we designthe optics system using AOTF, and take some measures to increase the light energyand the system SNR, which includes the three layers structure of the light chamber,the equipments to cover the useless light, polarizing to reduce the influence of 0 gradelight and focalize the light with lens. Apart from the hardware aspect, according to theexperiments, we can see that the different temperatures between at setting up themodels and at predicting the results will affect the prediction results in a way, and theaffection extent will be increased along with the increase of the temperaturedifference. Moreover, the light energy that can be detected will be different with thecontact pressure, and the match of the refractive indexes between the tissue and thesurroundings will enhance the light transmission depth, which will take moreinformation of the tissue. So, during the measurement, we should ensure the sametemperature between measuring and predicting, the same contact pressure at everymeasurement and the match of the indexes between the tissue and the surroundings aspossible as we can.

要提高测量精度，从硬件方面就必须有一个稳定的、信噪比较高的分光光路系统，以此得到稳定的近红外光来进行无创检测，针对这个目的，我们设计了 AOTF 分光光路系统，并在系统中采用一定的措施如三层灯室结构、遮光筒以减小杂散光、起偏和检偏来减小 0 级光影响、透镜汇聚等等来提高出射光的能量和系统的信噪比；从其他影响因素方面，通过实验，我们得到不同的建模温度对于预测结果有一定的影响，且影响随着温度的差值越大而越大，另外散反射光谱的能量也随着接触压力的不同而不同，组织的折射率与环境匹配将加大光的入射深度，从而能携带更多的组织信息，所以在测量时我们尽可能保证同一温度、保证同一接触压力以及利用物理或者化学的方法使组织与环境折射率匹配等。

The invention clones geranyl pyrophosphate synthase GGPPS gene from the ginkgo to construct plant expression vectors which contain ggpps gene, the ggpps gene is inducted into immature embryos of the ginkgo to induct out the callus tissue by the mediating of agrobacterium tumefaciens, PCR and semiquantitative RT-PCR detect the conformation and expression status of exogenous target gene ggpps, high-performance liquid chromatography and an evaporative light-scattering detector are employed to determine the terpene lactones content inside the callus tissue of the ginkgo, and the obtained callus tissue of the ginkgo of which the terpene lactones content is increased is screened.

本发明从银杏中克隆香叶基香叶基焦磷酸合成酶GGPPS基因，构建含ggpps基因的植物表达载体，用根癌农杆菌介导，将ggpps基因导入银杏幼胚并诱导出愈伤组织，PCR和半定量RT-PCR检测外源目的基因ggpps的整合和表达情况，高效液相色谱法及蒸发光散射检测器测定银杏愈伤组织中萜内酯含量，筛选获得银杏萜内酯含量提高的转基因银杏愈伤组织。

Results The results showed that the number of mast cells around the periphery of gingival cancer tissue increased significantly.

结果 在牙龈癌周围组织中肥大细胞显著增多，在组织化学上与正常牙龈组织的肥大细胞不同，不含肝素。

Objective To evaluate the role of CD44 in the pathogenesis of the glomerular diseases.

目的 观察CD44在胚胎肾组织和肾小球疾病患儿肾活检组织中的表达，以探明CD44在肾组织损伤发病机理中的作用。

Methods Realtime fluorescence quantitive RTPCR was used to detect the expression level of Tiam1 mRNA in gastric cancer tissues of 66 cases, paracancerous tissues (over 3cm) of 66 cases and gastric benign lesion tissues of 11 cases. Meanwhile, CK18 was selected as the hallmarker for lymph node micrometastasis to detect the level of CK18 mRNA expression in 199 lymph nodes from 24 cases of gastric carcinomas diagnosed negative by pathologic examinations.

采用TaqMan探针实时荧光定量PCR方法，定量检测66例胃癌患者癌组织、癌旁组织及11例胃良性病变组织中的Tiam1 mRNA拷贝数，同时将CK18作为淋巴结微转移的标志基因，定量检测24例病理诊断无淋巴结转移的胃癌患者199枚淋巴结的CK18mRNA拷贝数。

Sixty-three cases of AFP-positive gastric carcinoma were divided into four subtype: hepatoid type 22 cases (34.9％), fetal gastrointestinal type 15 cases (23.8%), and mixed type of the hepatoid type with enteroblastic differentiation to be 24 cases(38.1%), and yolk sac tumor-like type 2 cases (3.2％).

应用光镜、电镜、组化和免疫组织化学方法，对87例AFP阳性胃癌中临床病理资料较完整的63例进行病理组织形态观察，并用2例3个月妊娠的胚胎胃肠组织以同样方法行相关的对比研究。

Control of fusion and cell position are important parameters for creating functional heterotypic microtissues as well as the use of microtissues as building units to create larger tissue structures.

在创建有功能的异型微组织方面，以及把微组织当构建单位构建大组织器官的工作中，对融合和细胞定位的控制都是重要的参数。

The results show that the microstructure of as-cast Ti-45Al-7Nb-0·15B-0·4W becomes fineafter being HIPped and heat treated, and a more perfect lamellar microstructure is got. The temperature ofαphase

结果发现： Ti-45Al-7Nb-0·15B-0·4W的铸态组织经过热等静压和均匀化处理后，其晶团尺寸减小，层片组织更为完整； Ti-45Al-7Nb-0·15B-0·4W合金的α相转变温度为(1 290±5)℃； Ti-45Al-7Nb-0·15B-0·4W合金在超过1 295℃的温度进行热处理时，其显微组织由近层片

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失