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Our studies indicate that the increased contents of glucose and accumulation of active metabolic intermediates due to metaboic disorder contribute to the cutaneous environmental alterations associated with diabetes mellitus.Thus,the cutaneous environmental alteration is one of the initial events for impaired healing.The succession of progressive injury in deep partial thickness burn wounds is carried out depending on the physiological outcome of parabiotic tissues.The loss of dernal template due to the damage to the integrity and the continuity of dermal tissue may be one of the leading reasons of hyperplastic scar formation.

通过对糖尿病合并创面难愈、烧伤创面的进行性损害以及增生性瘢痕形成的机制研究,我们发现:糖尿病代谢紊乱所致皮肤组织中糖含量增高和代谢产物蓄积引起的皮肤微环境改变,是导致糖尿病创面难愈的始动因素之一;烧伤后的组织进行性损害的发生发展取决于&间生态&组织的病理转归;真皮组织缺损导致的模板作用缺失可能是引起增生性瘢痕形成的始动环节。

Methods The expression of caspase-3 and PCNA was detected by tissue microarray and immunohistochemical method in 95 hepatocellular carcinoma tissues,68 parahepatic cancer tissues and 19 normal liver tissues.

应用组织芯片技术和免疫组化方法检测95例肝细胞肝癌组织、68例癌旁组织和19例正常肝组织中Caspase-3和PCNA的表达。

Methods The expression of caspase-3 and PCNA was detected by tissue microarray and immunohistochemical method in 95 hepatocellular carcinoma tissues,68 parahepatic cancer tissues and 19 normal liver tissues.

目的 探讨Caspase-3和PCNA在肝细胞肝癌的表达及其在肝细胞肝癌发生、发展中的作用方法应用组织芯片技术和免疫组化方法检测95例肝细胞肝癌组织、68例癌旁组织和19例正常肝组织中Caspase-3和PCNA的表达。

Compared with those in control group,the number of positive immunoreactivity cell in RSR and intestine increased and its intensity of immunoreactivity enhanced obviously after rabbit was infected with Pasteurella multocida.

免疫组织化学观察发现,产抗菌肽细胞在兔圆小囊的黏膜上皮、圆顶上皮以及淋巴组织的滤泡生发中心、圆顶区和帽区均有分布;在兔感染多杀性巴氏杆菌后,圆小囊及其他肠道组织中的抗菌肽免疫组织化学阳性细胞数量明显增多,阳性反应增强。

The test result shows that the mechanical properties of the dual-phase structure are much higher than those of ferritic plus pearlitic structures, that the dual-phase structure is of better corrosion resistance in some cases for Ca_2 has corrosion-moderating effects, and that hot-rolling dual-phase structure of the low-carbon Mn-Si steel wit...

试验结果表明:双相组织的力学性能远高于通常的铁索体+珠光体组织;在一定条件下,双相组织具有更好的耐蚀性,Ca_2有明显的缓蚀作用;采用台阶淬火工艺,不含Cr、Mo的低碳锰硅钢也易于获得热轧双相组织

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色:翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成;基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质,在翼状胬肉体部深层基质中存在粗糙的纤维组织;正常球结膜组织细胞排列整齐;基质为疏松结缔组织,胶原纤维平行排列,其间可见成纤维细胞,散在少量中性粒细胞、毛细血管;Masson染色:翼状胬肉浅层基质中存在致密的胶原纤维染色,深层基质中的胶原纤维存在变性样改变;VG染色:翼状胬肉组织深层基质中存在大量变性的胶原纤维,其间夹杂黑色的弹性纤维;免疫组化染色法:MMP-3在翼状胬肉上皮细胞中呈强表达,成纤维细胞中呈中等强度表达,血管内皮细胞中未见表达;LN在血管壁中呈强表达,在上皮细胞基底膜中呈中等强度表达,在整个基质中未见明显表达;col Ⅲ在整个翼状胬肉基质中呈强表达。

Results Among the 149 patients of tuberculous pleuritis and pleural fluid, 16 patients in the thoracoscope group (n=19) were found to have granulation tissue, chronic inflammation and so on in their pleural tissue, which confirmed the histological changes of tuberculous pleuritis and the detection rate was 84.21%.

结果 胸腔镜组16例发现胸膜组织中存在肉芽组织和慢性炎症等,符合结核性胸膜炎的组织学改变,检出率为84.21%;经皮针吸胸膜活检组67例符合结核性胸膜炎的组织学改变,检出率为51.54%,两组检出率比较差异有统计学意义(P.05)。

Results The expression of MMP-9 was low in procerebrum at different phases in sham operated group.

结果假手术组各个时间段大鼠前脑组织少量表达MMP-9;缺血再灌注6h组大鼠脑组织即有MMP-9的表达,缺血再灌注24h组、48h组大鼠脑组织大量表达MMP-9;缺血再灌注3d、5d组大鼠脑组织亦可见MMP-9的表达,但较缺血再灌注24h、48h组相比MMP-9表达减少;除川芎嗪6h组不具统计学意义外,其它时间段川芎嗪治疗组与缺血再灌注组相比MMP-9含量均降低。

New productional organization adapt to the ability of the market demand uncertainty and advantage of efficiency that bacome to a dominant role of production organization.2、Technological innovation is the basement to the evolution of industrial organization.

新生产组织形式适应市场需求不确定性的能力和相对旧组织的效率优势,使其在本行业和其他行业及国家逐渐被模仿而不断扩散,最终成为占主导地位的生产组织。2、产业组织演化的基础是技术创新。

Methods: Forty adult Wistar rats were used for these operations of transplantation of frozen autogenous tooth-mandible compound and fresh ATMC. Then the healing process after transplantation of frozen ATMC and fresh ATMC was compared by clinical, radiographical, histological and microangiographical observation.

选用健康成年Wistar大白鼠40只,任取2只用于观察正常颌骨的组织学图像,其余的大鼠随机分为新鲜移植组和冷冻组,行牙-下颌骨复合组织块移植到胫骨区的手术,分别于术后1、2、3、4、6、8、12、16、20周时处死动物,采用大体观察、X线片、组织学及微血管造影等观察手段对牙-下颌骨复合组织块移植后的愈合过程进行比较。

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