组织

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

There have the thin cuticle and well-developed aquiferous tissues in the mature leaves of two species.

两种植物成熟叶均具较薄的角质层和发达的储水组织，叶肉组织中没有栅栏组织和海绵组织的明显分化。

This dissertation first introduced actuality of farmer specialty cooperation of Taizhou city in briefly and analyzed its characteristics from associator relation, organization structure, stock structure , benefit return and other sides. Indicated that these organizations have remarkable city characteristic of Taizhou; Sumed up main operative methodof Taizhou to develop farmer specialty cooperation as follows:(1) increased policy support, improved develop environment;(2) established service flat roof;(3) reinforced work lead, accelerated criterion development;(4) intensified organization impulse,formed develop main.

本文首先简要介绍了台州市农民专业合作社发展现状；从社员关系、组织结构、股权结构、利益返还和表权方式等方面分析了台州市农民专业合作社的组织特色，指出这些组织特色具有明显的台州地域特征；总结了台州市发展农民专业合作社的主要做法：(1)加大政策扶持，改善发展环境；(2)建服务平台，培植典型示范；(3)加强工作指导，促进规范发展；(4)强化组织推动，形成发展全力。

During the development of dental germ, enamel matrix proteins secreted by epithelial root sheath could induce dental follicle cells to differentiate towards cementoblast and form acellular cementum finally on the surface of tooth root. Many researches of domestic and oversea scholars suggest that enamel matrix proteins could effectively induce periodontal regeneration, and the structure and function of this regenerated tissue are similar to healthy periodontal tissue. Moreover, the regeneration process is nearly the same to that of embryonic development of periodontal tissue.

自发现釉基质蛋白可诱使牙囊细胞向成牙骨质细胞方向分化并在根表面形成无细胞性牙骨质以来，国内外学者做了大量研究，结果表明釉基质蛋白能有效诱导牙周组织的再生，且这种再生组织的结构和功能更接近于健康的牙周组织，并且再生过程也与牙周组织的胚胎发育过程类似。

Methods S-P immunohistochemical staining was used to detect the expression of VEGF-C and its reˉceptor Flt-4in10neighboring noncancerous tissue and60NSCLC cases of non-small cell lung cancer and32lymph nodes cases with lymph nodes metastasis of non-small cell lung cancer.

目的 探讨血管内皮生长因子C及其受体Flt-4在人非小细胞肺癌组织中的表达及其与淋巴结转移等临床病理特征的关系方法采用免疫组化S-P法检测60例NSCLC原发灶组织、10例癌旁组织、32例伴有肺癌转移的淋巴结组织中VEGF-C、Flt-4的表达，并将结果与肺癌的临床病理特征进行分析。

RESULTS: In the SH-rhBMP-2 group, sparse chondroid tissue could be found at the 2 weeks of operation, which arranged orderly along the tibial tunnel from 4 to 8 weeks, tendon adhered to adjacent tissue stably, and bony tissue was appeared at the 12 weeks.

结果：透明质酸钠-重组人骨形态发生蛋白2亚组在术后2周隧道内见稀疏软骨样组织，4~8周时隧道内见沿骨隧道排列的软骨样组织，双股肌腱之间形成稳定组织连接。l2周时出现部分骨样组织包裹肌腱。

Hepatic chymase concentrations in S3 and S4 cases were greater than in S1 and S2,〔53+54=(31.3±24.6)ng/mg vs. S1+S2=(5.7±4.8) ng/mg, P.01〕. Cells immunoreactive for chymase were seen throughout portal areas and intralobular sinusoidal walls, largely colocalizing with fibrosis. Chymase appears to be involved in hepatic fibrosis in chronic hepatitis.

慢性肝炎纤维化分期重的S3和S4患者，其肝组织中Chymase浓度〔S3+S4=(31.3±24.6)ng/mg〕明显高于纤维化轻的S1和S2患者〔S1+S2=(5.7±4.8)ng/mg〕，P.01；免疫组化染色结果，Chymase标记的肥大细胞主要分布于纤维化旺盛的汇管区与类洞壁，其分布与纤维化部位相一致，且肝组织中Chymase浓度高的患者，其肝组织内Chymase标记的肥大细胞分布增多推测肝组织中Chymase浓度可能与慢性肝炎肝纤维化关系密切。

With the help of Shapley value solution on coalitional game,the main organization forms of participating agricultural industrialization management including peasant household,peasant cooperative economic organization and cooperative interest distribution mode of company were studied.

我国由于农业发展处于计划经济向市场经济过度的阶段，还没有形成完善的组织体系，各种农业经营组织混杂存在，这些组织的存在必须依托农民福利的改进，因此，组织间合作形成有效的合作机制就成为值得研究的紧迫论题。

A number of research outcomes were concludedThe main conclusions: 1 the capital intensive, recourses intensive, scale-based benefit, high pollution and high industrial correlativeness are five most influential factors in paper industry; 2 theoretically, resources endowment, financing ability, quality of enterprise, structure of industrial organization, relevant industries, and the institutional environment are six key components of competitive power for regional paper industry. Among them, quality of enterprise, financing ability and the structure of industrial organization are the key elements for regional competition in paper industry; 3 the assessment of the competitiveness power of Fujian paper industry has showed that during 1999-2005, the competitiveness power of Fujian paper industry was stable; 4 financing ability and the structure of industrial organization have positive impact on the competitiveness power of paper industry; 5 The SWTO analysis shows that the advantage of Fujian paper industry is resource abundance, however, the disadvantages are financing ability and the structure of industrial organization.

主要结论有：①资本密集、资源密集、规模效益显著、污染型、产业关联度大等5个方面是造纸产业最为重要的5个特性；②从理论上分析，区域造纸产业竞争力的主要构成要素包括资源禀赋、融资能力、企业素质、产业组织结构、关联产业与制度环境等6个要素，其中企业素质、融资能力与产业组织结构是区域造纸产业的核心要素；③从福建省造纸产业综合竞争力的纵向测评结果来看，1999-2005年福建省造纸产业综合竞争力的表现较为稳定；④融资能力与产业组织结构对区域造纸产业竞争力具有显著的正向影响作用，它们是区域造纸产业竞争力最重要的影响因素；⑤根据SWOT分析结果表明，福建省在资源--对区域造纸产业竞争力影响有限的因素—拥有竞争优势，而在融资与产业组织结构等—对区域造纸产业竞争力有重要影响的因素-处于竞争劣势。

Results: IL-8 protein was located in the epithelium of viii. The expression of IL-8 was significantly increased in RSA group than that in the control group. Positive IL-8 cells was shown in decidua of RSA group, and its IL-8 level was higher than that in the control group. By hematoxylin-eosin staining. trophoblastic layer was found to get thinner, cells of trophoblastic layer denatured or necrotized, and turned acidophily, and the fibration of villous axis increased in RSA; decidual cells lost connection, a part of decidual cells appeared cytoclasis and turned more acidophilic, and nuclei disappeared in RSA.

结果：在绒毛组织中，IL-8蛋白定位于绒毛上皮细胞的细胞质内，且病例组的表达明显高于对照组；在蜕膜组织中；病例组蜕膜细胞的胞质内可见IL-8蛋白表达，且高于对照组；H-E染色可见病例组绒毛组织的滋养层变薄，细胞变性甚至坏死、嗜酸性增强，绒毛中轴纤维化程度增强；蜕膜组织中蜕膜细胞失去细胞间连接，部分蜕膜细胞解体、核消失。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！