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There has some disadvantages using these methods, including scarcity of donor site, keratinization and hair growth in transplanted skin, donor site morbidity.

组织工程为口腔粘膜缺损修复开辟了新途径,即应用人体少量的组织细胞经体外培养扩增后接种在支架材料上,移植到组织缺损处恢复组织的完整性。

The leafstalk, chief vein, bud, the cane top on side branch, bast and xylem of type B had type A and C tow components, belonging to chimeric tissues. The leaf pulp and thin leaf vein had no components of type C, belonging to pure tissues of type A. Type B plant had two kinds of color roots, the rufous roots belonged to type A tissue, the yellow roots belonged to type C tissue through the SSR identification.

深州杨B型的叶柄、叶主脉、芽、侧枝茎尖、韧皮部、木质部同时检测到深州杨A、C型两种成分存在,属于嵌合组织,B型叶肉及细叶脉中检测不到深州杨C型成分,属于深州杨A型纯合组织;深州杨B型植株的地下部分含有红褐色和土黄色两种根,经检测,红褐色根是属于深州杨A型的纯合组织,土黄色根是属于深州杨C型的纯合组织

The type and the size of epidermis cell, epidermis hair, stomata apparatus and inside blade tissue;Ascertaining by measuring the epidermis cell thickness of the stem, the type and density of the epidermis hair,the density of lenticel; the thickness of cortex, phellogen, vascular tissue and pith;And calculate the ratio of cortex and radius, the ratio of vascular tissue and radius, the ratio of pith and radius…….

测定出叶片表皮细胞、角质层和厚角组织厚度,表皮毛、气孔器和叶内组织的类型、大小等;测定出茎的表皮细胞厚度,表皮毛的类型和密度,皮孔密度,皮层、栓皮层、维管组织和髓的厚度,计算皮层与半径之比,维管组织与半径之比,髓与半径之比等。

It reflects functional peculiarity of conducting tissue by the lignification of cell wall and the specialization of the structure of cell wall in perforation,sieveplate ect.

细胞壁是包被在植物细胞原生质体外面的一个薄壁组织、机械组织、输导组织。各组织细胞细胞壁坚韧外壳,是植物细胞特有的

Methods The differentially expressed genes between liver tumor and non-tumor tissue were cloned by using suppression subtractive hybridization. Differentially expressed genes were sequenced and analized for homology in Gene Bank. In situ hybridization was performed to identify special expression of differentially expressed genes in livre tumor and non-tumor tissues.

方法通过抑制消减杂交法克隆土拨鼠HBV引发的肝癌组织和非癌组织中差异表达的基因;对差异表达基因进行DNA序列测定后于Gene Bank内分析其同源性;应用原位杂交确定差异基因在肝癌组织和非癌组织中的特异性表达。

B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.

体细胞胚胎发生研究的结果表明:(1)成熟过程中的合子胚是诱导水曲柳体细胞胚胎发生的最佳外植体材料;(2)在所试验到的MS、MS1/2(将MS的所有成分均减半)、WPM、DKW、B〓、SH等六种基本培养基中,MS1/2是最适合诱导水曲柳体细胞胚胎发生的基本培养基;(3)水曲柳的体细胞胚胎发生以直接发生为主,体细胞胚主要来自于从合子胚分离的完整子叶;(4)培养基中的激素组合对水曲柳的体细胞胚胎发生有显著影响,诱导直接体细胞胚发生较好的激素组合有NAA 1mg/L+IBA 2,5,10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0,2mg/L+TDZ 0.05mg/L;(5)合子胚分离的下胚轴具有最强的愈伤组织诱导潜力,少数愈伤组织可以分化出体细胞胚;(6)愈伤组织的诱导也受培养基和激素配比的显著影响,最适宜诱导的培养条件为MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2,5mg/L+TDZ0.05mg/L;(7)采种时间对体细胞胚胎发生有显著影响。7月末到8月中旬的合子胚具有较强的体细胞胚发生潜力,此时种子尚未成熟,胚乳已呈固态,种胚的各个部分已分化完全,种胚体积占胚腔的大约2/3;(8)在各自综合的最适条件下,完整子叶的体细胞胚诱导率可达33.68%,下胚轴的愈伤组织诱导率可达90.7%。

Of,relating to,or resembling lymphatic glands or lymphoid tissue.

淋巴组织的淋巴腺或淋巴组织的,与淋巴腺或淋巴组织有关的,类似于淋巴腺或淋巴组织

The positive expression both of p14ARF and mtp53 protein in NSCLC tissues were significantly different from that in nonmal lung tissues and tissues adjacent to the cancer (P.01).The expression of p14ARF protein was correlated with pathological degree(P<0.01),but it was uncorrelated with pathological staging,lymphaden metabasis and vessel invasion(P>0.05).The expression of mtp53 protein was correlated with clinical pathology staging,pathological degree and vessel invasion(P<0.05 or P<0.01),but it was uncorrelated with lymphaden metabasis(P>0.05).(2) The coexpression of p14ARF and mtp53 was 36.7%,their consistency was 38.8%,but the expression of p14ARF protein were negatively correlated with the expression of mtp53 protein(r=-0.3553,P<0.001).Conclusion The results suggest that the decrease of p14ARF and the increase of mtp53 protein may play an important role in the genesis and development of NSCLC.

结果 (1)在正常肺组织、癌旁组织和NSCLC中p14ARF蛋白的阳性表达率分别为93.3%、83.3%、55.1%,mtp53蛋白的阳性表达率分别为6.7%、44.4%、79.6%;p14ARF蛋白和mtp53蛋白在NSCLC中的表达与正常肺组织和癌旁组织比较均有显著性差异(P<0.01);p14ARF蛋白表达与临床病理分期、有无淋巴结转移、有无脉管浸润无关(P>0.05),而与病理分化程度有关(P<0.01);mtp53蛋白表达与临床病理分期、病理分化程度(P<0.01)及有无脉管浸润(P<0.05)有关,而与有无淋巴结转移无关(P>0.05);(2)在NSCLC中,pl4ARF与mtp53蛋白共表达为36.7%,一致性为38.8%,二者表达呈负相关(r=-0.3553,P<0.001)。

One gene was methylated in 12 of HCC(30%). Two genes were methylated in 5 of HCC(12.5%).

CHFR基因在癌组织和癌旁组织的甲基化率分别为52.5%和27.5%,癌组织中的甲基化率高于癌旁组织(P.05)。

The plastic embedding techniques and the Leica SP1600 saw microtome could be widespread utilized in the study of histomorphology and the osseointegration surface of the oral implant.

塑料包埋技术适用于不脱钙硬组织包埋,制作含金属硬组织切片,可以广泛应用于含金属硬组织组织形态学研究和口腔种植体的骨整合界面研究。

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If you have any questions, you can contact me anytime.

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