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Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖,琼脂0.7%,pH5.8 培养基中25℃暗培养20d 后继代一次,诱导产生Ⅱ型胚性愈伤组织,用镊子将其夹碎成2mm2大小的小块,置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中,侵染时间为45min,共培养3 天后,转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天,再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中,遮光培养20d后,置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化,卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%;同时以甘蔗心叶愈伤组织材料,通过循环水式真空泵,产生负压,设定不同的负压值,在农杆菌菌液中感染5min 后,在负压条件下继续侵染15min 后,筛选出抗性愈伤组织并获得转化植株,其中在负压为-0.05 时转化率达到最大值,其卡那霉素抗性愈伤组织获得率平均为37.5%。

Several regulations are provided in Taiwan to be against those crimes, such as "The Law of Racketeer Influenced and Corrupt Organizations Act","The Money Laundering Control Act.","Communications Surveillance Act". But in fact, these regulations are not as appropriate as they supposed to be. Accordingly, the aim of this dissertation is to review these regulations and provide some suggestions for emending these regulations in Taiwan with comparative studies preferring the related regulations of United State and Japan.

我国对於组织犯罪活动的防制已订有组织犯罪防制条例、洗钱防制法、通讯保障及监察法等相关规定,惟相关法制的运作仍有诸多不甚妥适之处,因此,本论文拟仅就我国现行法制下抗制组织犯罪所采行之通讯监察、洗钱防制与扩大没收等制度,进行一系列之回顾与检讨,此外,本论文亦将参酌美、日诸国抗制组织犯罪之相关法制经验进行比较研究,并藉此提出相关法制修正建议,以期强化我国抗制经济型组织犯罪之成效。

"The three basic weave s are plain or tabby (weft threads go over one warp thread, then under one), twill, and satin. Fancy weave s, such as pile, Jacquard, dobby, and leno, require more complicated looms or special loom attachments."

织物的三种基本组织是平纹、斜纹和缎纹,花式组织有起绒组织、提花组织、小提花组织和纱罗等,这些需要更复杂的织布机或特殊的附件才能完成。

L-citrulline was determined with liquid scintillometer, and the activity of NO synthase(induced NOS, total NOS, constitutive NOS) in pulmonary and right ventricular homogenates was counted.

采用比色法测定血浆、肺组织、右心室组织一氧化氮代谢产物(NO2-/NO3-)的含量,液体闪烁仪测定[3H]-瓜氨酸的生成量,计算肺组织、右心室组织组织型NOS(tissue NOS,tNOS)、诱导型NOS(induced NOS,iNOS)和原生型NOS(constitutive NOS,cNOS)的活性。

Histological and cellular research on rachis of healthy and inoculated spikes of the mutants at the different stages showed that, structure and developed tendency of cortical sclerenchyma tissue and green tissue and fibrovascular tissue in the rachis were alike between the mutants and their donors at anthesis stage, which also showed no apparent difference in the inoculated spikes'rachis between the mutants and their donors, major difference was that the mutants had less hyphae number than their donors.

对抗赤霉突变体的健康穗及接种穗的穗轴细胞组织学研究结果显示,抗性突变体与其感病亲本的花期健康穗轴在皮层厚壁组织、绿色组织及维管组织结构上基本一致,不同发育时间的变化趋势也一致;接种处理后,抗性突变体与其感病亲本的穗轴组织在各方面的变化趋势相一致,变化量也无明显差异。

Healthy spikes of high-resistant varieties had thicker cell wall and tissue of cortical sclerenchyma, more number of cortical sclerenchyma layers, more number of fibrovascular bundles, smaller area of green subcutaneous tissue in rachis, and theses differences, except number of fibrovascular bundles, enlarged over time. Considering inoculated spikes, cell wall and tissue layers of cortical sclerenchyma displayed different, and to how much extent the relationship between this difference and anti-extension ability of variety had not determined. 6. On the analysis of agronomical characters and molecular marking technology, more than 30 alien anti-Gibberella wheat materials and two mutants coming from this experiment were evaluated their hereditary multiplicity.

实验结果还证明,高抗品种和感病品种的穗轴组织结构确实存在一定差异,在健康穗中,主要表现在高抗品种的皮层厚壁细胞壁和皮层厚壁组织较厚,皮层厚壁细胞层数较多,维管束数目较多,穗轴表皮下绿色组织面积较小,抗、感品种间的差异达显著水平,随发育时间延长,高抗品种的皮层厚壁细胞壁厚度、厚壁组织厚度和厚壁细胞层数增加的幅度较大;在病穗中,主要表现在高抗品种的皮层厚壁组织细胞层数和厚壁细胞壁厚度增加的幅度较大。

The result showed that the expression of Bmhsp19.9 was detected in all kinds of tissues, but fluctuated greatly in different tissues. The expression was abundant in lively tissues, such as testis, ovary, silk gland and pupae.

Bmhsp19.9在各组织中均有一定量的表达,但不同组织间的表达差异达10多倍,表达量丰富的组织是生长发育旺盛的组织,如精巢和卵巢,然后是丝腺、蛹等组织

Related theories and practices show that only starting from the double-win of both organizations and members can real member identification be motivated. The system of member stockholding is important to improve member identification and organizational administrative integration.

相关理论和实践表明,只有以组织和员工双赢为出发点的组织管理整合才能激发真正的员工组织认同,员工持股制度则是促进员工组织认同和增强组织管理整合有效性的重要制度。

After 24 weeks,the operation area of group A was more smooth,and the surrounding normal cartilage naturally straight flush,transparent form new cartilage,subchondral bone formation in good condition;Group B restoration surgery the basic integrity of the cartilage tissue, center is not yet fully integrated,there was slight depression;CollagenⅡimmunohistochemistry of cartilage that was new brown area.Group C has no formation of articular cartilage.The growth and the intergration of subchondral bone of group A and B were better.

术后24周取材,见A组山羊手术区关节表面较为光滑,与周边正常软骨自然连续平齐,透明的新生软骨组织形成,软骨下骨形成完好;B组山羊手术区修复的软骨组织基本完整,中心部位仍未完全融合,有微小凹陷;Ⅱ型胶原免疫组化示新生软骨组织呈棕黄色。C组术区关节凹陷,无关节软骨组织形成。A组和B组,软骨下骨的生长及与周围组织的结合均较好,无植入物脱落现象的发生。

By the method of empirical research, the relationship was analyzed among the leadership behavior, organizational learning capability, and organizational performance. The conclusions could be drawn as followed:(1)The three kinds of leadership behavior of TRC model have significant effect on the organizational learning capability, in which the most important is change-oriented, the second is relation-oriented, and the third is task-oriented according to the significant.(2) Organizational learning capability has a complete mediate effect on the relationship between relation-oriented, change-oriented leadership behavior and organizational performance.

本文通过对领导行为、组织学习能力与组织绩效三个不同层面的变量之间关系进行实证研究,得出如下主要结论:(1)企业家的TRC三种导向领导行为均对组织学习能力有显著影响,其中变革导向第一,关系导向行为第二,工作导向行为第三;(2)组织学习能力在关系导向、变革导向领导行为与组织绩效的关系起完全中介作用。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。