组氨酸

Objective To study the relationship between aberrant FHIT transcripts and hepatocellular carcinoma.

目的 研究脆性组氨酸三联体基因异常转录与肝细胞癌的相关性。

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

Because the shell albuminoid can decompose histidine,lysine etc 18 types of amino acids and taurine through the biological enzyme of digestion liquid and sweat liquid in human body (equivalent to the chemical catalyst).

因为壳角蛋白通过人体中的消化液和汗液中的生物酶的作用可分解为组氨酸、赖氨酸等18种氨基酸及牛磺酸。

Materials and methods: The composite was respectively prepared by blending Lysine, Histidine, Arginine with PLGA, while the composite respectively prepared by blending Algin, chitosan, NaHCO3 with PLGA as the comparison and the pure PLGA as the control. All composites were degradated in tri-distilled-water at 37℃ for 7 weeks. The degradation property of PLGA with the addition of basic additives was investigated by using gross observation, the pH change of degradation solution and the mass loss ration of the composite.

材料方法：将赖氨酸、组氨酸、精氨酸分别各按5%和10%的比例与PLGA制成复合物，于体外在37℃下在三蒸水中进行降解2个月；通过总体观察、降解液PH的变化、复合物的失重率等指标来考察碱性氨基酸对PLGA体外降解特性的调节作用，同时将碱性氨基酸的调节效果与海藻酸钠、壳聚糖、碳酸氢钠的进行对比。

The levels of all identified metabolites are notably higher at day 11 and day 14. At day 21, the amounts of Asn and His were decreased, but Val, lactate, Ala, malate, succinate, GABA, choline,β-glucose, fructose, allantoid, Phe and formate were all increased. Enteric bacteria must overcome the acid environments in host organs to colonize the host.

结果表明：根瘤菌处理5d后苹果酸含量显著增加，7d时丙氨酸浓度降低而异亮氨酸、苹果酸、琥珀酸、GABA、胆碱、磷酸胆碱、β-葡萄糖、延胡索酸、组氨酸、苯丙氨酸、色氨酸含量显著增加。11d和14d的差别极为显著，所有已鉴定代谢物的含量都大大增高。

Irreversibly inhibits trypsin but not chymotrypsin by alkylating the histidine residue in the active site of the enzyme.

通过乙酰化酶活性位点剩余的组氨酸，不可逆的抑制胰蛋白酶。

Under optimal conditions mixtures containing eight inorganic anions, six amino acids such as arginine, histidine, cysteine, methionine, tryptophan and proline with positive or negative charge and four basic proteins such as lysozyme, cytochrome c,α-chymotrypsinogen A and myoglobin were separated completely.

经优化，用中等浓度的中性缓冲液分别成功地分离了八种不同的阴离子Br〓、NO〓、NO〓、SCN〓、MoO〓、BrO〓、WO〓和IO〓；六种带不同电荷的氨基酸如精氨酸、组氨酸、半胱氨酸、甲硫氨酸、色氨酸和脯氨酸；四种碱性蛋白质如溶菌酶、细胞色素c、抗凝乳蛋白酶原A和肌球蛋白。

Catalytic autoxidation of AA was inhibited by stable Cuamino acid complexes; histidine, having the highest conditional stability constant for its Cu-complex, showed the strongest inhibitive effect.

稳定之含铜氨基酸复合物会抑制抗坏血酸之自氧化；组氨酸对铜离子复合物有很高的稳定性，且具有很强之抑制作用。

To detect recombinant MUC1 with histag by flat-bed isoelectric focusing and provide reference for purification of recombinant MUC1 without histag by ion exchange chromatography.

目的 采用薄层等电聚焦方法检测含有组氨酸尾的粘蛋白表位疫苗（简称重组MUC1），为不含有组氨酸尾的粘蛋白表位疫苗的下一步离子交换层析纯化蛋白提供参考依据。

Total RNAs from KAx-3 cells and AK127 cells(developed for 14h) were isolated. After the reverse transcription and PCR reaction, two distinct differential fragments were acquired., fragment A was from KAx-3 cells and fragment C was from AK127 cells. After retriving and reamplifying the differentially expressed fragments, white-blue plaqueselection, the fragments were purified. Northern blot proved that fragment A was from KAx-3 cells and fragment C was from AK127 cells. The results of sequencing and researching for NCBI database have been showed: part sequence of fragment A shows 91% similarity to the gene encoding DhkA, 92% similarity to the gene encoding DhkF, 91% similarity to the gene encoding STATc, 97% similarity to the homoeobox gene encoding protein. These genes play important part in controlling cell differentiation and cell proportion in Dictyostelium discoideum.

本研究通过提取盘基网柄菌发育14小时的野生型KAx-3细胞和突变型AK127细胞的总RNA，运用mRNA差异显示法分离出了两条明显的差异表达片段，其中片段A来自野生型KAX-3细胞，片段C来自突变型Ak127细胞；并通过凝胶回收差异片段、对差异片段进行再次PCR、蓝白斑筛选克隆、提取质粒、酶切电泳纯化差异片段；接着进行Northern杂交的结果表明，片段A只与野生型KAx-3细胞的总RNA有杂交信号，片段C只与突变型AK127细胞的总RNA有杂交信号，这就排除了差异片段假阳性的可能；最后通过测序，搜索NCBI BLAST数据库发现：片段A的小部分序列与编码组氨酸激酶DhkA基因中一段序列的相似性高达91%，与编码组氨酸激酶DhkF基因中的一段序列相似性高达92%，与编码STATc蛋白基因的一段序列相似性达91%，以及与编码同源框蛋白的基因中的一段序列相似性达97%，这些基因在盘基网柄菌细胞分化和细胞比例调控过程中起着相当重要的作用，这些数据进一步说明了突变细胞不能完成发育的原因。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。