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组氨酸

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Histidine – The main sources of histidine are radishes, carrots, cucumber, beetroots, celery, garlic, onions, turnips, alfalfa, spinach, pineapples, apples, pomegranates and papaya.

组氨酸-的主要来源氨酸是萝卜,胡萝卜,黄瓜, beetroots ,芹菜,大蒜,洋葱,萝卜,苜蓿,菠菜,菠萝,苹果,石榴和木瓜。

The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物,经过扩增前导序列10~34aa基因序列,并重新克隆入质粒pRSET-A构建串联二聚体后,再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点,经一系列简单的酶切和连接,快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因,并成功表达其六聚体重组蛋白。

Results:Urine excretions of 3-MH increased,and protein and glutamine concentrations of skeletal muscle decreased significantly when sepsis occurred.

结果:感染后尿3-甲基组氨酸排出量明显增加,同时肌肉蛋白及谷氨酰胺含量明显下降。

The recombinant proteins were purified with affinity columns by the 6 histidines tag or Glutathione-S-Transferase they were fused to.

并根据所表达融合蛋白的6个组氨酸或谷胱甘肽-S-转移酶标记进行亲和纯化。

Coli. The protein was expressed in a soluble form and as a fusion protein. There was a 6 histidines tag at its amino acid terminus. It was purified by immobilized metal ion affinity chromatography under native conditions.

所表达的蛋白是一种可溶性的融合蛋白,其氨基端带有6个组氨酸的标签,因此可以用固定化金属离子亲和层析法在非变性条件下进行纯化。

The HμOR overexpressed in this study, with a tag of six consecutive histidines at its carboxyl terminus, will facilitate the future studies on exploration of reconstitution and structural basis of ligand recognition of HμOR.

我们在HμOR的C-端锚定六个连续的组氨酸(6His),将有利于HμOR的分离纯化,研究HμOR的重组和配体识别的结构基础。

It is a very basic, single-chain protein with molecular weight about 14 kD. It shares 33% sequence identity with bovine pancreatic RNaseA and has structurally equivalent counterparts for the two histidines and one lysine that comprise the catalytic residues for ribonucleolytic activity.

它是由123个氨基酸组成的分子量约为14kD的单链碱性蛋白质,与牛胰核糖核酸酶有33%的同源性,两个组氨酸和一个赖氨酸残基组成了血管生成素核糖核酸酶活性中心。

There were 6 histidines linked to the N-terminal of targeted protein which was expressed by pRSETA-B7-2-PE40KDEL vector, which facilitated the purification process by using Ni-NTA affinity chromagraphy.

由于本研究构建的pRSETA-B7-2-PE40KDEL表达载体所表达的目的蛋白N端融合有6个组氨酸残基标记,这十分利于通过Ni-NTA金属鏊和层析来进行表达产物纯化。

The codons encoding a tag of six consecutive histidines was added to 3'-end of human μOR cDNA by polymerase chain reaction with primer pair 5'-AATACG ACTCACTATAG-3' and 5'-AAAGGTCGACTAATTATCATTAGTGATGGTGATGG TGATGGGGCAACGGAGCAGTTTCTGC-3'.

本研究通过PCR方法在人μ阿片受体cDNA的3'端锚定编码六个连续组氨酸的密码子,PCR产物亚克隆人质粒载体pBluescriptⅡKS+/-的Hind Ⅲ/Sal Ⅰ位点。

Objective: Construct a lentiviral expression vector containning a scavenger receptor that binds uniquely phosphatidylsedne and oxidized lipoprotein with the six histidines tag in order to investigate the function of SR-PSOX in atherosclerosis.

目的:通过构建结合磷脂酰丝氨酸和氧化脂蛋白的清道夫受体与6个组氨酸融合基因慢病毒表达载体,诱导其表达,为研究SR-PSOX在动脉粥样硬化中的作用提供技术平台,探讨该基因是否通过TNF-α而参与AS的形成过程。

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