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组氨酸

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Anti-histidine-tag antibody is usually used for identification of expression of the recombinant protein expressed using pRSET-A. A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence.

质粒pRSET-A是一个常用的高效原核表达载体,编码一N端含组氨酸标签(6×His)的34aa前导肽序列,以方便利用抗组氨酸标签抗体鉴定或纯化所表达的重组蛋白。

A series of L-histidine Schiff bases that include Sal-His (H2L^1), Br-Sal-His(H2L^2), NO2-Sal-His (H2L^3) and their Cu complexes were synthesized, and the catalytic behavior of Schiff-base-Cu for the oxidation of β-ionone to 4-oxo-β-ionone as well as the effects of substitutents on these properties were examined.

合成了芳环上含不同取代基的Schiff碱配体(水杨醛缩组氨酸(H2L^1)、5-溴水杨醛缩组氨酸(H2L^2)、5-硝基水杨醛缩组氨酸(H2L^3)及其铜配合物,研究了其作为催化剂应用于催化分子氧氧化β-紫罗兰酮制备4-氧代-β-紫罗兰酮中的行为。

Shanghai Kyowa Amino Acid Co., Ltd. is a Sino-Japan joint venture found ed by Guanshengyuan Co.,Ltd and Kyowa Hakko Kogyo Co.,Ltd.

现公司最新供应以下产品: L-丙氨酸、L-精氨酸、L-盐酸精氨酸、L-门冬氨酸、L-谷氨酸、L-组氨酸、L-盐酸组氨酸、L-盐酸赖氨酸、L-脯氨酸、L-丝氨酸、L-苏氨酸等。

Comparing with the structure of heme group that covalently linked to the polypeptide chain in natural heme proteins, we simulated the covalent modification of artificial Fe-salophen complex with amino acid histidine or dipeptide cysteinyl-histidine, with the intention of providing useful information for design of metalloproteins containing nonnatural prosthetic group.

通过比较天然血红素蛋白中血红素与多肽链进行共价连接的结构,我们对组氨酸或二肽半胱氨酰-组氨酸共价修饰人工配合物Fe-salophen进行了计算机模拟,旨在为设计包含非天然辅基的金属蛋白分子提供有用的信息。

The gold electrode is available in determination of purine and pyrimidine bases.

实验优化了组氨酸在氧化铝修饰碳糊电极上吸附及进行还原反应的条件,探讨了组氨酸在氧化铝修饰碳糊电极上的还原机理。

The cancers studied here were throat cancer, aute non- lymphoblas He leukem ia and acut lymphoblastic leukemia. The result s indicated that,(1)the levels of Leu, Lys, Ser Asn,Ile,Gly,and Thr were obviously decreased whea lse the levels of orn, Trp evidently increased in cultural liquid of throat can cer cell line indicating that the growth of HepⅡ cell consumed 7 amino acids and releaced 2 amino acids into cultural liquid.(2)the levels of Glu, Gls, Leu, Phe, Tyr and Trp were evidently increased while the levels of Thr , His, Ala decreased in plasma from patient with ANLL. These r esults were consistent with the previous report.(3)the levels of Gly, Trp and Phe in plasma from patients with ANLL evidently de creased and the levels of Ala, His increased after therapy indicating that cance r cells were growing in an anaerobic catabolism state.

这些癌肿包括喉癌HepⅡ细胞,急性非淋巴细胞白血病和急性淋巴细胞白血病,结果表明:(1)喉癌细胞株培养过程中亮氨酸、赖氨酸、丝氨酸、天冬酰胺、异亮氨酸、甘氨酸以及苏氨酸等水平明显降低,而色氨酸水平明显增加,说明喉癌细胞的生长繁殖必须依赖以上7种氨基酸同时释放了色氨酸;(2)急性非淋巴细胞白血病患者血浆中的谷氨酸、甘氨酸、亮氨酸、苯丙氨酸、酪氨酸和色氨酸等水平明显升高,而苏氨酸、组氨酸、丙氨酸等水平明显降低,这些结果与国际报道相一致;(3)经治疗后,ANLL患者血浆中甘氨酸、色氨酸和苯丙氨酸等水平明显降低,而丙氨酸、组氨酸等水平明显升高,表明肿瘤细胞处在无氧代谢。

The enzyme histidine decarboxylase catalyses the conversion of histidine into histamine by removing the a-carboxylate group of the substrate.

这种酶组氨酸脱羧酶催化成通过消除组胺的基板1 -羧酸组的组氨酸转化。

Sheep tibetan antelope by wool horn albumen the composition of amino acid and goat, sheep by wool fibre identical; but there is certain difference on the content scale of all sorts of amino acid, acid content of ammonia of group of cashmere tibetan antelope is 5.88%, all the acid content of ammonia of content; guang of acid of ammonia of the group in prep above cashmere wool and continous abb is 10.60%, alkalescent and amino acid and acidit

藏羚羊被毛角蛋白氨基酸的组成和山羊、绵羊被毛纤维相同;但在各种氨基酸的含量比例上有一定的差异,藏羚羊绒组氨酸含量为5.88%,均高于山羊绒和绵羊毛中组氨酸的含量;胱氨酸含量为10.60%,高于其它所测山羊的绒和毛;碱性氨基酸和酸性氨基酸含量接近(14.87%,15.23%);藏羚羊绒促螺旋蛋白含量低于降螺旋蛋白。

It was observed that not only histidine, but also histidine-Co and histidine-Ni could induce and substitute some Cu and Zn from Cu_2Zn_2SOD, eventually influence the activity of this metalloenzyme. Histidine- metal ion complexes have stronger ability than histidine to displace the bound metal ion from SOD. In addition, the amount of foreign metal ion binding to the SOD was less than that of metal ion lost from SOD. It obviously means that there was apoenzyme in the solution.

实验结果表明: Cu2Zn2SOD酶不仅与组氨酸,而且还可以与组氨酸-CoⅡ)和组氨酸-Ni(Ⅱ相互作用,Cu2Zn2SOD酶中的部分Cu和Zn被诱导和置换出来,并相应地影响了酶的活性;组氨酸-金属离子配合物的作用比组氨酸强,酶活性降低也更明显;酶中结合的外来金属离子的量比从酶上被诱导和顶替下来的金属离子的量要少,说明在溶液中有脱辅基酶的存在。

To detect recombinant MUC1 with histag by flat-bed isoelectric focusing and provide reference for purification of recombinant MUC1 without histag by ion exchange chromatography.

目的采用薄层等电聚焦方法检测含有组氨酸尾的粘蛋白表位疫苗(简称重组MUC1),为不含有组氨酸尾的粘蛋白表位疫苗的下一步离子交换层析纯化蛋白提供参考依据。

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