组因子

METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

无菌抽取的骨髓经肝素化后，采用密度梯度离心法及贴壁筛选法分离获得人骨髓间充质干细胞，传至第5代按1× 108 L-1接种于6孔板内，设立2组，依达拉奉组细胞达50%融合时用含碱性成纤维生长因子、胎牛血清的L-DMEM预诱导24 h，PBS洗涤后再用20 mg/L依达拉奉无血清L-DMEM诱导24 h；空白对照组始终用含体积分数为10%胎牛血清的L-DMEM培养，不加任何预诱导剂和诱导剂。

The morphological changes: The morphological observation of the aorta section showed that, in model group, space in subendothelial layer area widened, and there presented many small vacuoles and even large vacuoles in some areas.

血清中炎性因子含量的变化：CRP和TNF-α是早期炎症反应的两种较为敏感的指标，本实验观察到，随着实验天数的增加，模型组和预防组大鼠血清CRP含量均急剧上升。与实验前相比，实验开始后10天、17天以及24天的CRP含量均明显升高（P.05）。

Cells in the induction group were treated with L-DMEM consisting of 10% fetal calf serum and 10 μg/L basic fibroblast growth factor for 24 hours, then induced with L-DMEM consisting of 2% dimethyl sulphoxide and 200 μmol/L butyl hydroxy anisol for 1, 3 and 5 hours.

设立2组，诱导组用含10%胎牛血清和10 μg/L成纤维生长因子的L-DMEM培养基预诱导24 h后，再以含2%二甲基亚砜和200 μmol/L丁羟基茴香醚的L-DMEM无血清培养基诱导1，3，5 h。

Groups of 16,20mg significantly increased the level of hormones of serum, such as insulin,growth hormone, triiodothyronine(T3),thyronine(T4except the group of 16mg),insulin-like growth factor-1 (IGF-1),but there was no significant effect on the level of serum glucose.3 , All dosage can significantly increase the crude protein(except the group of 20mg) and significantly decrease crude fat (p.05);and they had significanteffect on some parameters of body stature, such as length of body slope, length of chest bone, girth of chest, pelvic breadth, metatarsus length, metatarsus girth.

不同剂量的G98均能提高肉鸡的激素水平，其中16、20mg/Kg的两个剂量组能显著性提高血清中胰岛素、生长激素、类胰岛素生长因子-1(IGF-1)、三碘甲腺原氨酸(T_3)、甲状腺素(T_4，16mg/Kg剂量组除外)的水平；但对血糖水平没有显著性影响。

The cells survival rates of every group were determined by MTT technique.Results The tumor cells without any anti-glioma factor treatment were growing better and no change was observed in morphology.After 2-3 days,cell axons disappeared,cell bodies crimpled,nucleoli crinkled and cracked in each treated group.

结果 未加任何抗癌因子处理的C6细胞组肿瘤细胞生长旺盛，细胞形态无明显改变；各处理组的肿瘤细胞2～3天后开始逐渐出现细胞突触消失，细胞变圆，胞体皱缩，核仁固缩、裂解、甚至消失，继而逐渐死亡。

To observe the morphologic changes of deciduas and progesterone receptor expression in deciduas of early unexplained spontaneous abortion patient with immunohistochemistry staining.

同时，在体外培养细胞时，再加孕酮刺激的为P组，未加孕酮刺激的为NP组，用于观察孕酮在不明原因自然流产时对患者PBMC产生细胞因子的作用。

METHODS 10 mm long segmental defects were created in the diaphyses of both radii in 16 New Zealand rabbits. The defect was bridged with a silicon tube. Human recombinant aFGF was instilled into the tube on the experimental side, while the contralateral tube was instilled with saline as control group.

16只新西兰白兔分为四组，每组 4只，造成兔双侧桡骨干10mm节段性骨缺损，以硅胶管桥接骨缺损，实验侧管内置入人基因重组酸性成纤维细胞生长因子24μg，对侧管内注入生理盐水作对照。

In all clinical features, there were no significant differences between the De-Bil and In-Bil groups by the chi-square test.

胆红素下降组和胆红素上升组在所有的临床因子上，都没有显著的差异。

Using single-factor design, 300 AA broilers, 1-day-old, were randomly divided into 6 groups with 5 repetitions.

方法］采用单因子设计试验，选用300只1日龄AA肉鸡，随机分为6组，每组设5个重复。

METHODS: After the mouse PBMCs were isolated and cultured in wells, which contained glass coverslips and ivory dentine slices, macrophage colony stimulating factor and osteoprotegerin ligand were added.

将外周血单核细胞分组培养，在A组中加入巨噬细胞集落刺激因子和OPGL ，B组中再加入地塞米松。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。