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E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.Results:Myocardial collagen in model group increased obviously compared with normal group from the results of electron microscope、H.E、Sirus red in supersaturated picric acid solution.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型,随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组,并设正常对照组,用药四周后处死动物通过透射电镜观察心肌细胞超微结构;HE染色、苦味酸天狼星红染色观察心肌胶原数量;放免法测定血清透明质酸的含量;免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

Methods:Establish the model of myocardial fibrosis in chronic stage of viral myocarditis by injecting CVB3 into Balb/c rats,divide these rats into model group、Tong xin luo low dose group、Tong xin luo medium dose group、Tong xin luo high dose group and set up normal group,after four weeks of giving medicine execute the animals and observe the ultrastructure of myocardial cell with tramission electron microscope,the quantity of myocardial collagen was observed by staining with H.E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型,随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组,并设正常对照组,用药四周后处死动物通过透射电镜观察心肌细胞超微结构;HE染色、苦味酸天狼星红染色观察心肌胶原数量;放免法测定血清透明质酸的含量;免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

[Objective]To study the relativity during polyethelene debris cytokine and loosening of hip prosthesis.[Methods]Thirty eight patients with loosening of hip prosthesis were divided into three group:total hip arthoplasty,double hip arthoplasty and integrated arthoplasty.The inflammative reaction was analyzed with light microscopic observation arrording to the half-ration standard of Joseph,and the content of TNF in samples of interface membranes was determined.

[目的]研究聚乙烯微粒、细胞因子与人工髋关节松动的相关性[方法]根据假体的类型及假体中是否含有聚乙烯将38例人工髋关节翻修病例分 3组:全髋置换组、双动头组和体式单动头组,对各组术中松动假体周围界膜组织进行光镜下炎性细胞计的半定量分析和肿瘤坏死因子的测定,分析人工髋关节无菌性松动与聚乙烯微粒、细胞因子之间的相关关系。

Methods The SAD group and non-SAD group were measured with LASA by clinician-scoring and self-scoring respectively.

设SAD组和健康人组,分别进行LSAS的他评和自评,对有关数据进行t检验、x^2检验,重点比较两组因子分的性别差异。

MethodsThe transplanted tumor model of the mouse S180 sarcoma was established.Fifty mice were randomly divided into five groups,the control group,the CTX group,the Gecko group.They were treated respectively with oral administration of saline,and intraperitoneal injection of CTX 100 mg/kg only one time,oral administration of Gecko in doses of 13.5,9,4.5 g/kg,one time a day.After 14 days,the anti-tumor activity was evaluated by tumor tissue weighing. The impact on immune organ was detected by accounting thymus index, spleen index and the number of peritoneal macrophage which phagocytose chicken red blood cells.The protein express of vascular endothelin growth factor and basic fibroblast growth factor were detected by SABC immunohistochemistry.

方法建立移植瘤小鼠S180肉瘤模型,将50只雌鼠随机分为对照组、环磷酰胺组、壁虎高组、中组、低组共5组,分别给于生理盐水灌胃1次/d,CTX(100 mg/kg)腹腔注射1次,壁虎高、中、低组(13.5,9,4.5 g/kg),每天灌胃1次。14 d后,称取荷瘤小鼠瘤重、胸腺重、脾脏重,计算抑瘤率、胸腺指数、脾脏指数,观察腹腔巨噬细胞吞噬鸡血红细胞实验指标;用SABC免疫组化法检测血管内皮生长因子、碱性成纤维细胞生长因子的蛋白表达,TUNEL方法检测细胞凋亡率。

Objective To study the mechanism in which the personality, copying style influenced survival time by changing immunity function in the patients with gastric cancer Methods The Minnesota Multiphase Personality Inventory, coping style questionnaires and immunity function were measured in patients with gastric cancer on late stage, gastric cancer patients with longer survival time, and normal control Results Psychopathic deviate, social introversion subscale scores of MMPI in groupⅡ were significantly lower than that in the other two groups ( P 05) Statistical difference were found between groupⅠand groupⅡ in problem saving factor and help seeking factor of CSQ ( P 05) Natural killer cell level in groupⅡ was significantly higher than in groupⅠ( P 05) There was negative correlation between the NKC and depression, psychopathic deviate, paranoia, psychasthenia, schizophrenia subscale scores of MMPI, there was positive correlation between NKC and SP, coping type in patients with gastric cancer Conclusion The gastric cancer patients with longer survival time are more extroversive and have more mature coping styles Their NKC level is relatively higher The results may be advantageous to improve the prognosis of gastric cancer

作者中文名:王健;邹义壮;唐丽丽;李杰;张东;金山;刘娟摘要:目的研究胃癌患者的生存期与个性特征、应付方式等心理因素,以及免疫功能的关系。方法对胃癌晚期患者、胃癌较长生存期患者及正常健康人分别进行明尼苏达多相个性测验、应对方式问卷、免疫功能测试,并做相关分析。结果(1)MMPI:胃癌Ⅱ组人格障碍评分(42.0±10.5)及社会内向评分(42.2±11.6),低于其他两组[胃癌Ⅰ组为(49.3±11.2)分和(48.3±9.4)分,对照组为(48.6±10.9)分和(39.9±11.5)分;P均<0.05];(2)CSQ:解决问题和求助因子分,胃癌Ⅰ组[分别为(0.68±0.27)分和(0.42±0.18)分]均低于胃癌Ⅱ组[分别为(0.82±0.18)分和(0.65±0.21)分;P<0.05和P<0.01],胃癌Ⅱ组更多采取成熟的应对方式;(3)自然杀伤细胞:胃癌Ⅱ组水平(21.4±9.2)高于胃癌Ⅰ组[(14.2±8.8);P<0.05];而NK细胞水平与MMPI的抑郁(r=-0.35)、人格障碍(r=-0.39)、偏执(r=-0.29)、精神衰弱(r=-0.30)及精神分裂(r=-0.30)等量表呈负相关,与CSQ的解决问题因子(r=0.35)及应付方式类型(r=0.34)呈正相关。结论与胃癌Ⅰ组比较,胃癌Ⅱ组更为外向,负性情绪较少,更多采取成熟的应对方式,其NK细胞水平较高,对胃癌的预后产生了积极的影响。

Two defects ofarticular cartilage were made in the trochlear groove of each rabbit. The defect of groupA was filled with stabilized fibrin-chondrocyte constructs that had BMP-2 and that ofgroup B was filled with the stabilized fibrin-chondrocyte constructs.

在A组、B组和C组的关节软骨缺损中分别植入有生长因子BMP-2的细胞改良FG支架复合物、无生长因子的细胞改良FG支架复合物以及无细胞和生长因子的单纯改良FG支架,D组为空白对照组。

Whippets with a single copy of this mutation are generally very fast; those with two copies have disproportionately large musculature and are known as "bully whippets".

有一组因子突变的惠比特通常奔跑非常迅速。而那些两组因子突变的惠比特就会发育出不成比例的硕大肌肉组织,而被成为&公牛惠比特&。

Thirty SD rats were randomly divided into five groups: control, 1 week, 2 week, 3 week and 4 week and stimulating the right triceps surae was stimulated by electrical stimulator. Using real-time quantitative PCR measurement to measure two alternative splices of IGF-1(IGF-1Ea and MGF) and MyoG mRNA expressing of gastrocnemius and right biceps brachii.

SD大鼠30只,随机分为5组,每组6只,分别为对照组和1、2、3、4周电刺激组,刺激部位为右侧腓肠肌,实时荧光定量PCR法分别检测双下肢腓肠肌和前肢肱二头肌的胰岛素样生长因子-1(IGF-1)不同变构体肝型胰岛素样生长因子(IGF-1Ea)和机械力生长因子以及成肌分化因子(Myogenin, MyoG)mRNA表达。

Following successful modeling, rats of bFGF group were intratracheally injected with 400 U bFGF and rats of VEGF group with 2 μg VEGF, once a week for three times. MSCs group was injected 1 mL suspension of 4×109/L MSCs into tail vein. MSCs+VEGF group was injected MSCs into tail vein and intratracheally injected VEGF (2 ug, three times) at the same time. Model control and normal control groups were intratracheally injected with equal volume of sodium chloride.

成功造模后,碱性成纤维细胞生长因子组气管内注入400 U碱性成纤维细胞生长因子,血管内皮生长因子组气管内注入2 μg血管内皮生长因子,1次/周,共3次;单纯细胞移植组于尾静脉注入4×109 L-1骨髓间充质干细胞悬液1 mL;血管内皮生长因子+细胞移植组气管内注入血管内皮生长因子的同时,尾静脉注入骨髓间充质干细胞;模型对照组、正常对照组给予相同体积的生理盐水。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。