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The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维织增生;HE染色可见B、C、D三给药后四周及八周时硬膜内外均有炎细胞浸润,纤维细胞轻度增生,硬膜外神经根内有炎细胞浸润及炎性渗出;特殊染色B、C、D三同A相比未见有脊髓及神经根的改变;免疫化染色,给药后四周及八周时,硬膜内外均有Ⅱ型胶原纤维增生;固兰染色B、C、D三未见有明显脱髓鞘改变,与A相比无明显异常改变;电镜观察B、C、D三在给药后的四周及八周时,表现为硬膜外神经根内髓鞘厚薄不一,形状不规则,可见成纤维细胞,神经内膜中可见有巨噬细胞;粗大的有髓神经纤维变化明显,无髓神经纤维受累较轻;八周时电镜下D改变较B、C两为轻。

Our experiment establish OA animal mode by causing high pressure in bone, and the therapy is the three united remedy that combined by the fumigate of Chinese materia medica、 the terminal stimulus and the massage.

采用随机的方法将56只4—10月龄健康日本大耳白兔分为7个,正常、模型、三联法、熏洗、功能锻炼、手法弹拨、扶他林,每8只。

Can accelerate the bone marrow cell from G1 phase into S phase when we measure it's Cell cycle, it make S phase rate was increased .thus it promoted DNA synthesize and repaired. Mice irradiated at dose of 5.5 Gy gamma-irradiation showed timed-related decreases and restores in peripheral blood picture at day 1 through day 21, the counts of WBC of these group had taken drug has various degree to increase than irradiation control group in day 1. Grugs failed to protected the peripheral blood cell at lowest point, but seems promote their rescovery.9803 treated group was distinct. At 7days after mice were radiated in 3.5 Gy. we observed drug can Significantly hold back the decreased number of hematopoietic progenitors colony forming radiation induced. These findings indicated 9803 have a certain radioprotective activity against gammer irradiation in mice.

给药的 CFU-S 数量较照射对照明显增加,3.5Gy 照后第七天观察给药9803对小鼠骨髓造血细胞集落生成能力的影响,给药的造血祖细胞集落的形成能力强于照射对照,并且9803明显优于523。5.5Gy 照射引起的小鼠的外周血细胞数量的降低以 WBC 发生最早,照后24小时给药的 WBC 数量均比照射对照有不同程度的升高,以9803各给药最为明显,药物未能使照射引起的外周血细胞最低值升高,而有降低的趋势,但给药对照射后期外周血象的总体恢复有较好的促进作用。7.5Gy 照射后第七天测定小鼠骨髓细胞周期的测定中发现9803具有促进骨髓细胞由 G1期进入 S 期的效应,致使 G0/G1期细胞显著减少, S 期细胞比率显著增加,这利于促进 DNA 合成修复,即促进骨髓细胞增殖,骨髓细胞 DNA 含量给药显著高于照射对照

The comparison of blood loss and operation score between two neighboring groups both showed no significance. Also, we found significant difference between group4 and group 7, as well as group5 and group 7 in their operative time comparison (P=0.045 and 0.028, respectively). No complications but a slight subcutaneous emphysema happened on the 21 cases.3. When the electronic pulse-wave mode insufflator is working, IAP of patients may reach 40mmHg, more than twice of the setting point, 15mmHg.

相邻两的手术时间比较为第5与第6间差异有显著性(P=0.034),相邻两的出血量比较差异均无显著性(P值分别为0.273,0.607,0.731,0.494,0.863,0.494),相邻两的手术得分比较差异均无显著性P值分别为0.761,0.729,0.344,0.873,0.074,0.816非相邻别的手术时间比较为第4与第7、第5与第7间差异有显著性(P值分别为0.045,0.028。

At the same time,the morphological variations of the ampulla and ithmus of the oviducts were observed and measured by light microscopy. Results The serum levels of estradiol were E>PE>ME>DE>OVX.The differences between the two groups were significant.The height of the oviductal epithelia was E>PE>DE>ME.After ovariotomy,the epithelia of the oviduct were atrophied and regressive.

结果 大鼠血清雌二醇浓度依次为:动情期>动情前期>动情后期>动情间期>去卵巢,各间差异均有显著性;输卵管粘膜上皮高度在动情周期中变化为:动情期>动情前期>动情间期>动情后期;去卵巢后,输卵管粘膜上皮萎缩。

Methods seventy-two 15-week-old sd rats were divided into six groups,included control group and five ovariectomized model groups.the model groups were fed with different-dose lycopene[10,15,20 mg/] for 12weeks.then related indexes including length bone mineral density,bone mineral,serum estradiol,sertum alp and uterus index were analyzed.results compared with model group,three different dose groups and nilestriol[1.05mg/] group showed increased bone mineral density,bone mineral,serum estradiol and uterus index,but decreased serum alp (p.05).conclusion as a female sex hormone-like component,lycopene may ameliorate the bone quality and inhibit osteoporosis in ovariectomized rat.

将72只15周龄sd大鼠随机分为6,空白做假手术,其余5做卵巢切除术,术后用药12周后处死,测定右侧股骨骨密度、骨矿含量、血清雌二醇、碱性磷酸酶含量及子宫湿重。结果番茄红素高[20mg/]、中[15mg/]、低剂量[10mg/]、尼尔雌醇[1.05mg/kg·bw]能对抗骨密度、骨矿含量、血清雌二醇的下降、血清碱性磷酸酶的升高,与模型比较,p.05;番茄红素各剂量、尼尔雌醇能对抗子宫指数的减轻,与模型比较,p.05。结论番茄红素可以改善骨质量,抑制大鼠去卵巢骨质疏松的发生。具有类似雌激素的效应。

Results: HE stain showed the obvious thicken of the artery, RV/ and mPAP elevated obviously (P.05) in model group in comparison with the control group, they were also elevated obviously (P.05) in the model group compared with the treatment group. The tryptase + mast cell and the chymase + mast cell obviously increased in the model group compared with the control group, also increased in the model group compared with the treatment group.

平均肺动脉压力模型动物较对照明显升高(P.05),模型较治疗也明显升高(P.05);右心室/模型较对照明显增加(P.05),模型较治疗也明显增加(P.05),肥大细胞总数(类胰蛋白酶阳性的肥大细胞)及类糜蛋白酶阳性的肥大细胞模型较对照明显增多(P.05),肥大细胞在治疗较模型明显减少(P.05)。

Methods: 50 normal Wistar male rats were randomly divided into five geoups: the sham operated group, injury model group, shenjindan capsule treat group, high dose and low dose group (duzhongyaotong pill treat, D and E)(n=10each). Autogenous nucleus pulposus was removed from the coccygeal vertebral and placed into the back of left L5~6 nerve root, The modle of the non-compressive nucleus pulposus protrusion was created by transplanting autogenous nucleus pulposus in the back of left L5~6 nerve roots. At tow weeks, the SP in dorsal root ganglion was determined by immunohistochemistry.

取健康Wistar大鼠50只,随机分为假手术、模型、对照、高剂量(D、腰腿理痛散高剂量)、低剂量(E、腰腿理痛散低剂量),每10只,将大鼠自身的尾椎髓核取出移植于左侧硬膜外腔L5、L6神经根背侧,造成非压迫性大鼠自体髓核移植硬膜外模型,通过免疫化的方法测定大鼠2周时DRG中CGRP的变化。

Methods: 1. Rats in the study were divided into six groups: normal control group, hyperlipidemia group (fed with high-cholesterol diet), atherosclerosis group (vitamin D〓 intramuscular injection, endothelium denudate and fed with high-cholesterol diet with vitamin D〓), L-arginine group (besides treatment as atherosclerosis group, 2.25% L-arginine was added in the drinking water), chitosan group (besides treatment as atherosclerosis group, 5% chitosan was added in the diet), simvastatin group (besides treatment as atherosclerosis group, 0.03% simvastatin was added in the diet). The study lasted 90 days.

1。实验大鼠分为6:正常对照、高脂血症、动脉粥样硬化(维生素D〓肌肉注射加动脉球囊损伤手术加含维生素D〓的高脂饲料饲养)、L-精氨酸(动脉粥样硬化基础上饮水中加入2.25%L-精氨酸)、几丁糖(动脉粥样硬化基础上饲料中加入5%几丁糖)、辛伐他汀(动脉粥样硬化基础上饲料中加入0.03%辛伐他汀),90天后取标本检测。2。

Portal vein pressure, size of spleen the collateral circulation of portal vein and the fluctuation of hematocyte counts were measured and recorded at the preconcerted time.

实验犬25只,随机分为A(正常,n=5)、B(对照,n=10)、C(实验,n=10)。A仅予以剖腹探查,B予以门静脉主干部分结扎建立模型,C则通过门静脉主干部分结扎附加3周后脾静脉缩窄及其属支结扎的方法建立模型,建模后术前及术后每周动态观察两动物血象的变化,B、C在预定时间点(术后第5、第9周)分别随机选择5只动物开腹观测门静脉自由压、脾脏大小、门体侧支循环形成情况,并切取脾脏行织病理学检查,此外,术前及术后预定时间点了解骨髓增生情况变化。

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