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Expression of nNOS mRNA, eNOS mRNA and iNOS mRNA in the E 20 cerebral cortex: There were no obvious changes of expression of nNOS mRNA in ETU-injected and control groups. Expression of eNOS mRNA increased significantly in cerebral cortex of the encephalocoele group, And there were no obvious changes of expression of eNOS mRNA in cerebral cortex of the control group and the other ETU-injection groups.

六、不同E20胎鼠大脑皮质中nNOS mRNA、eNOS mRNA和iNOS mRNA的表达 1、实时定量PCR检测发现对照、给药无畸形、脊柱裂和脑膨出E20胎鼠大脑皮质中nNOS mRNA表达无明显改变; 2、给药无畸形、脊柱裂和对照胎鼠大脑皮质中eNOSmRNA表达无明显改变,而脑膨出胎鼠大脑皮质中eNOS mRNA表达明显增多; 3、在各未检测到iNOSmRNA表达。

In the control group, encephalorrhagia and the treated group (encephalorrhagica+nimodi-pine), premature ventricular beat were (3±5) thousands/d,(14±16) thousands/d,(7±7) thousands/d, respectively.

结果:对照、脑出血和脑出血+尼莫地平治疗3大鼠的室性早搏分别为(0.3±0.5)万个/d、(1.4±1.6)万个/d和(0.7±0.7)万个/d,尼莫地平治疗与对照比较无显著性差异(P>0.05),上述2与脑出血比较有显著性差异(P<0.05、P<0.01)。

In Comlex model group the endangium plaque were widespread, while in that of High cholesterol group there was only single small plaque. The pathological heart changes of both High cholesterol group and Complex model group weren't obvious, with mild oedema and small fat drops. The liver cells of High cholesterol group showed mild oedema, while fat drops in the liver of Complex model group were more typical.

病理显示主动脉弓变化明显,复合模型内膜斑块弥漫,而单纯高脂内膜只出现单个小斑块,单纯高脂和复合模型心脏病变区别不大,均见轻度水肿和小脂肪滴;单纯模型肝脏细胞轻度水肿,而复合模型肝脏脂肪滴明显。

Based on pre-experiment foundation of the earlier period, 60 male patients among 45-65 year old were chosed as the clinical case according to the PADAM diagnosis standard, and were divided into 3 groups according to the block stochastic principle, the observation group (acupuncture combined with BUSEHN capsule), group 1, group 2 (takes orally BUSEHN capsule group), each group had 20 cases, and each group of patients was measured by the method of radio-immunity for the serum level of testosterone, luteinizing hormoha, follicle-stimulating hormone before and after the treatment. By the contrast of the grade of PADAM symptom among three groups before and after treatment ,we can reveal the whole funcamental characteristic of acupuncture combined with herb ,and discuss a new way to enhance the curative effect of acupuncture ,and summarize the endocrinous adjustment of acupuncture for the PADAM's patients and the orchidic improvement by the treatment of acupuncture.

在前期预实验的基础上,选择符合诊断标准的PADAM诊断标准的45-65岁男性患者临床病例60例,按照区随机原则分为3,观察、对照1、对照2,每各20例,采用放射免疫法分别测定各患者治疗前后血清中血清睾酮、黄体生成素、卵泡刺激素的血清浓度的水平;通过三及三间治疗前后PADAM症状评分指数的对比研究,揭示针药结合的整体调节作用特色,探讨提高针灸疗效的新途径;总结出针刺对中老年雄激素缺乏综合征患者的内分泌的调节及针刺对睾丸的改善程度,探讨针药结合治疗本病的机制及针灸增效剂的应用前景,为今后进一步研究打下坚实基础。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. The n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule.

取健康SD大鼠60只,正常对照和正常银杏叶治疗各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水,治疗A(每日EGb 50mg/kg)、治疗B (每日EGb 100mg/kg)、治疗C (每日EGb 150mg/kg)、治疗D (每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. Then 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照和正常银杏叶治疗各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水,治疗A(每日EGb 50mg/kg)、治疗B (每日EGb 100mg/kg)、治疗C (每日EGb 150mg/kg)、治疗D (每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. The n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照和正常银杏叶治疗各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水,治疗A(每日EGb 50mg/kg)、治疗B (每日EGb 100mg/kg)、治疗C (每日EGb 150mg/kg)、治疗D (每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照和正常银杏叶各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水,治疗A(每日EGb 50mg/kg)、治疗B (每日EGb 100mg/kg)、治疗C (每日EGb 150mg/kg)、治疗D (每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数来评估神经元的情况。

Difference was significant. Serum LDL-C levels in D group were higher than thoes in ErD group.

血清胰岛素D>ErD>C>E,D与三之间差异均具有显著性。

objective to study the effect of qingshanjianxin tablet on treating arrhythmia.methods sixty wistar mouse were ransdomed into six groups, aconitine evocate arrhythmia, and continuously observe the changes of electrocardiogram and record the latent period, the lasting time of the arrhythmia and the death rate.results qingshanjianxin tablet can prolong the latent period of the arrhythmia, shorten the lasting time of arrhythmia and lower the death rate.

目的 研究青山健心片抗心律失常的作用机制。方法采用乌头碱诱发wistar大鼠心律失常,持续观察心电图变化直至出现心律失常,记录心律失常的潜伏期、持续时间及死亡率。结果青山健心片高、中剂量均能延长心律失常的潜伏期,缩短心律失常的持续时间,降低死亡率,与心律平片无明显差异(p>0.05),疗效优于低剂量、空白对照、心律宁片(p<0.05),高剂量与中剂量之间无显著差异(p>0.05)。

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