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RESULTS : The hypertrophic cardiomyocyte NTPase Vmax values at 1 week group and 4 week group were all increased significantly in both of ATP and GTP as substrates with much higher argumental extent in early hyper group; their Km values were kept constant in early hyper group and were decreased only in late hyper groups.

结果:早、晚期肥大心肌细胞核被膜NTPase最大反应速率以ATP为底物时较对照增加25%~62%(P<0.01),以GTP为底物时增加8%~44%(P<0.01),其中晚期肥大NTPase最大反应速率增加幅度低于早期。早期肥大细胞核被膜NTPase Km值不变,晚期NTPase Km值下降,以ATP和GTP为底物时晚期肥大Km值分别下降22%和86%。

Methods] Healthy SD rats were divided randomly into 6 group, including: normal control group(sham-operation), ischemia for 45 min (reperfusion 0h, n=6), reperfusion 2,4,12 and 24 hr group. Renal ischemia reperfusion injury models were founded in other five groups, opened abdominal and placed atraumatic vascular champ in dual kidney pedicle for 45 minutes,followed by reperfusion 2,4,12 and 24 hr.

方法] 将SD大鼠随机分成正常对照、单纯缺血、再灌注2 h、4 h、12 h、24 h,制备双侧肾蒂夹闭模型后,腹主动脉取血测血肌酐、尿素氮,行肾脏织病理学检查,免疫化法检测TGFβ1 蛋白,RT-PCR法检测TGFβ1 mRNA。

Methods: Screens 983 example metabolism syndrome patient from the immortal river area healthy physical examination staff, the blood sugar unusual 675 examples will carry on the grouping, branches out the pure IFG group, the IFG+IGT group and diabetes group through the OGTT experiment (has explicit diabetes medical history not to carry on the OGTT experiment), conducts the intervention research to the pure IFG group 117 examples, divides into the life style intervention group, the life style add two armor biguanide intervention group and the control group, after the research intervention treatment, this part of patient's extension to turn over to the situation.

从仙河地区健康查体职工中筛选出983例代谢综合征患者,将其中血糖异常的675例进行分,通过OGTT试验分出单纯IFG、IFG+IGT和糖尿病(有明确糖尿病病史者不进行OGTT试验),对单纯IFG117例进行干预研究,分为生活方式干预、生活方式加二甲双胍干预和对照,研究干预治疗后这部分病人的转归情况。

Methods Healthy rats were divided into 3 groups, group A as control group; group B as caecal ligation plus puncturegroup and group C as trioxymethylanthraquinone group.

将健康大鼠随机分为三,A:假手术;B:盲肠结扎穿刺;C:CLP大黄素治疗

The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 130dB infrasound treatment In 1 day group, Bcl-2mRNA and P53mRNA were expressed lightly in CA1-CA3 areas in hippocampus and dentate gyrus. Blood vessels dilated lightly. In 7d group, Bcl-2mRNA and P53mRNA were more expressed in areas mentioned above. Dilation of capillaries and small vessels was more obvious. Bcl-2mRNA and P53mRNA were strong positive in endothelial cells of vessels. In 14d group, Bcl-2mRNA and P53mRNA expression was obviously increased in pyramidal cells of hippocampus and granular cells of dentate gyrus, showing deep blue staining. a The endothelial cells in some vessels were also strong positive for Bcl-2mRNA and P53mRNA. Dilation of capillaries and small vessels was lessened. In 21d group, Bcl-2mRNA and P53mRNA expression was decreased in pyramidal cells. In 28d group, little change about the expression and distribution of Bcl-2mRNA and P53mRNA was observed. Lots of Bcl-2mRNA and P53mRNA were scattered in cortex of temporal lobe around hippocampus and callosal gyrus area. Dilation of capillaries and small vessels was lessened obviously. The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 90dB infrasound treatment.

次声作用后Bcl-2mRNA和P53mRNA在海马的表达,16Hz、130dB次声作用1d:两者在海马〓区和齿状回轻度表达,海马内血管轻度扩张;7d:两者表达在上述区域较1d增多,微血管和小血管扩张较显,血管内皮细胞内可见呈强阳性表达的Bcl-2mRNA和P53mRNA;14d:海马锥体细胞和齿状回颗粒细胞内Bcl-2mRNA和P53mRNA阳性表达明显增多,呈深兰色,在部分血管内皮细胞内两者强阳性表达,微小血管扩张状态较7d减轻;21d:锥体细胞胞浆内Bcl-2mRNA和P53mRNA表达减弱;28d:上述表达及分布与21d相比无明显改变,海马周围颞叶皮层和扣带回区域亦可见大量散在分布的Bcl-2mRNA和P53mRNA表达,血管扩张状态已明显减轻。90dB次声作用1d-7d后,较对照无明显变化,14d-21d后轻度表达,28d后,Bcl-2mRNA和P53mRNA表达较强。

Results①In non-vibration group, the peak of temperature appeared at operation 3min; in vibration group,the temperature were increasing gradually as the peak appeared at evacuating 3min .②vibration group had better significant and persistent calorigenic effect than non-vibration group. ConclusionVibration massage apparatus has the validity of heightening temperature.

结果①不同测量时间对受试者局部温度有影响(P.05),空置中温度升高在空置3 min后即刻尤为明显,尔后温度呈逐渐下降趋势,而振动中随着时间的增加,背部温度不断升高,在振动后撤离仪器3 min达到峰值;②空置和振动都能使局部皮肤温度有不同程度的升高,但振动比空置产热效应更显著,而且更为持久。

Results:the time of vim convalescence, draining, antibiotics use, fervescence, intention, hospitalization were all shorter in the presacral space clysis group than that in the tradional treatment group.the times of dressing wound and the number of secondary suture and c healing were all smaller in the presacral space clysis group than that in the tradional treatment group.and the infection rate of the former was 0.136 times of the latter.

结果:骶前冲洗的精神状态恢复时间、血浆引流管保留时间、抗生素使用时间、体温升高时间、伤口愈合时间、住院时间均短于传统处理,骶前冲洗的换药次数、伤口二期缝合例数、伤口丙级愈合例数均少于传统处理,以及骶前冲洗的伤口不良愈合几率是传统处理的0.136倍。

The result of observation under microscope: Model group has a little inflammation nature cells to soak; Astragali many candy high dosage have inflammation nature cell soak, can see angle take layer; When Chinas dosage heals, the epidermis structure has already basically taken shape, has had a large number of inflammation nature cells; It is collagenic that there are new students in the low dosage group.

综上所述,创伤6d以后,对照的伤口面积要明显小于模型,愈合率要高于模型,中级量的愈合率要高于模型,中剂量愈合速度最快,高剂量在12d愈合率也高于模型

Methods Eighteen majority medical fitness mongrel canines were randomly divided into three groups: Group A (gastrojejunostomy or colocolic anastomosis + CHIP), Group B and group C (only gastrojejunostomy or colocolic anastomosis).

将成年健康杂种家犬18只随机分为三:A(胃空肠吻合术或横结肠吻合术+腹腔热灌注)、B和C,每6只。

Methods:108 cases of 7-day-old sprague-dawley rat were divided at random into 3 teams : hypoxic-ischemic-team (hi team,36 cases),false-oprational control team(control team 36 cases), hypoxic-ischemic-gm1(gm1 team,36 cases).deligation of cephalic artery and breathing in low-oxygen gas mixture was applied to prepare hibd mode.

选择新生7日龄sprague-dawley大鼠108只,随机分为三:缺氧缺血(hi,36只),假手术对照(control,36只),缺氧缺血+神经节苷脂(gm1,36只)。

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