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3NE、MTT、LDH、ET-1、ICAM-1 and Tie-2experiment show: in the process of oxygen glucose deprivation/reoxygenation ,BMEC have subjected great damagement, I/R groups have significant differences compared with NC groups (p.05);polymorphonuclear leukocyte can aggregrate this injury, I/R+PMN groups have significant differences compared with I/R groups (p.05); after Sivelestat Sodium treatment, the effect of I/R injury has been decreased, I/R+PMN+Siv groups have significant differences compared with I/R+PMN groups (p.05), and NE、MTT、LDH experiment show that the three dose groups there was a significant dose-effect relationship.

3测定NE、MTT、LDH、ET-1、ICAM-1、Tie-2 mRNA均显示:氧糖剥夺/复氧复糖过程能对BMEC造成损伤,I/R与NC比较有明显的统计学差异(p.05);中性粒细胞能加重对BMEC的损伤,I/R与I/R+PMN之间比较亦有统计学差异(p.05);西维来司钠处理后,血管内皮细胞的损伤明显减轻,I/R+PMN与I/R+PMN+SIR比较有统计学差异(p.05),并且NE测定以及MTT、LDH实验提示在西维来司钠三个剂量之间存在明显的量-效关系,其对BMEC的保护作用有剂量依赖性。

At endpoint, every cardiac function parameter in group MSCs and TXL was not significantly different compared with group control (all P>0.05), however, the number of dyskinetic segments (P=0.039) and infarcted size (P=0.0061) in group simvastatin, and the number of dyskinetic segments (P=0.04) in group atorvastatin, were remarkably decreased compared with group control.

终点时,MSCs、通心络超微粉和对照无显著差异(47.3±13.2%,44.8±13.9%vs 47.8±11.1%,P>0.05),而辛伐他汀和阿托伐他汀灌注缺损面积均小于对照(39.3±12.4%,38.7±13.1%vs47.8±11.1%,P<0.05);尤其是通心络超微粉+MSCs、辛伐他汀+MSCs、阿托伐他汀+MSCs的灌注缺损面积进一步显著减小(27.6±7.4%,28.3±7.7%,29.3±9.0%vs 47.8±11.1%,P<0.05)。

Compared with control,①the mean tumor weight of H22 of SXKA Granules three dose groups were decreased significantly(P.01,P.05),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 30%;②the mean tumor weight of S180 of SXKA Granules three dose groups were decreased significantly(P.01),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 32%;③the mean tumor weight of EAC of SXKA Granules three dose groups were decreased significantly ( P.01, P.05),and the mean inhibition rates of SXKA Granules 20 g/kg dose groups were above 38%;④the mean tumor weight of Lewis carcinoma of SXKA Granules three dose groups were decreased significantly(P.01, P.05),and the mean inhibition rates of SXKA Granules 20、10、5 g/kg dose groups were above 36%;⑤the mean tumor weight of W256 of SXKA Granules three dose groups were decreased significantly ( P.01, P.05),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 32%;⑵Compared with control,SXKA Granules 20、10 g/kg dose groups had extended the survial time of the P388-bearing mice respectively(P.01),and the mean prolong rates of SXKA Granules 20、10 g/kg dose groups were above 50%;⑶Compared with S180-bearing group, SXKA Granules 20、10 g/kg dose groups could increase the weight of thymus and spleen, Spleen index and thymus index were increased, SXKA Granules 5 g/kg dose group could increase thymus index(P.05);⑷As Compared with control group, SXKA Granules 20、10 g/kg dose groups could improve mouse serum half hemolysis value depressed by transplanted tumor dramatically(P.01), which revealed the SXKA granules could improve the mouse humoral immunity system;⑸SXKA Granules 20 g/kg dose group could increase of englobe indexαon S180-bearing mice remarkably(P.01), which indicated the SXKA Granules could improve their cellular immunity system.

对荷W256大鼠,生兴克癌冲剂20、10、5 g / kg三的平均瘤重明显低于对照(P.01,P.05),生兴克癌冲剂20、10 g / kg的平均肿瘤抑制率均大于32 %;⑵与空白对照相比,生兴克癌冲剂20、10 g/ kg能显著地延长移植小鼠白血病P388小鼠的存活天数(P.01),生兴克癌冲剂20、10 g/ kg对荷白血病P388小鼠生命延长率均在50%以上;⑶与S180荷瘤相比,生兴克癌冲剂对荷瘤鼠的免疫器官重量、胸腺指数和脾指数有一定的提高趋势,其中生兴克癌冲剂5 g / kg对荷瘤小鼠的胸腺指数有一定的提高作用(P.05);⑷与S180荷瘤相比,生兴克癌冲剂20、10 g /kg可提高由荷瘤引起的小鼠血清半数溶血素值的降低(P.01),表明其可提高荷瘤小鼠体液免疫功能;⑸与对照相比,生兴克癌冲剂20 g /kg可提高荷S180肉瘤小鼠的免疫吞噬系数α值(P.01),表明其可提高荷瘤小鼠细胞免疫功能。

Deference was significant. 2. Levels of serum TC, TG and LDL-C in D group were higher than those in C group. Difference was significant. Levels of serum TC, TG and HDL-C in ErD group were higher than C group.

血脂代谢:D大鼠TC、TG、LDL-C均高于C,差异具有显著性;ErD大鼠TC、TG、HDL-C均高于C,差异具有显著性;LDL-C水平:D>ErD>C,三之间差异均具有显著性。3。

Three basin prototype s are generated in the southeast of Junggar Basin: filling basin in the post-rift sequenceP_2-T_(2-3,foredeep basin in the post-rift sequenceT~~3__3-J_(1-2 and intracontinental foreland basin(J_3-E).

准噶尔盆地东南部发育了3套盆地原型,即P2—T2-3的后裂谷期充填盆地、T33—J1-2的裂谷后前渊盆地、J3—E的陆内前陆盆地的并列叠加作用;发育了3套主力生油岩系,即上二叠统芦草沟、红雁池和上三叠统黄山街、郝家沟及下、中侏罗统八道湾、三工河、西山窑,并赋存于阜康凹陷、吉木萨尔凹陷、五彩湾凹陷、黄草湖凹陷之中,形成了多个供油中心。

Methods]ninety femal sd rats,weight 230~280 gram,were rangdonmized into three groups: qiangguyin group,miacalcic group and control group.all rats were variectomized.after 10 weeks,the osteoporosis model succeed,the treatment group,contrast group were given qiangguyin,miacalcic respectively.the treatment group used qiangguyin everyday,1ml/g.the contrast group used miacalcic 0.72u/kg,everyday hyodermic.and 3,4.5,6 months after the treatment,kill 10 rats to explore.the neck of femur were cut and stined with masson golder trichrome for bone histomorphomemetric contrast group analysis.

方法]健康雌性sd大鼠90只,体重230~280g。随机分为3,分别为治疗、对照和空白,每30只。分别采取切除双侧卵巢方法进行骨质疏松造模,10周造模成功后,开始给药,治疗用自拟强骨饮灌胃,每日一次,每次1ml/g,对照用密盖息,皮下注射,每日1次,每次0.72u/kg。空白,不做处理。在给药后3、4.5、6个月每各取10只大鼠进行检测,先测量体重,空气栓塞处死,分离股骨颈,获得股骨颈样本,经切片等处理后,显微镜下作骨形态计量学检测。

It was found by means of protein fluorescence detection that the number of MAP-2 masculine neurone in the hippocamp of AD rat in model group and zinc reinforce post-AD group decreased distinctly. There was not distinct difference in the MAP-2 expression and the number of MAP-2 masculine neurone between normal group and zinc reinforce pro-AD group.

模型和AD后补锌大鼠海马区MAP-2蛋白表达与正常大鼠相比显著下降,MAP-2蛋白荧光检测发现模型和AD后补锌大鼠海马区MAP-2阳性神经元数量明显减少,AD前补锌大鼠海马区MAP-2蛋白表达和MAP-2阳性神经元数量与正常相比差异无显著性。

Results: Both Pur and Hup-A improved learning memory ability of I/R mice, the effect of Pur group was better and the effect of combination group was the most significant. Both Pur and Hup-A decreased neuron apoptosis and raised content of monoamine nervous transmitter of I/R mice. The cortex neuron apoptosis rate in combination group was lower than that in Hup-A group,the homovanillic acid level of cerebral cortex in combination group was higher than that of Pur group.

结果:Pur(120 mg/kg)或Hup-A(400 μg/kg)都可提高I/R小鼠的学习记忆成绩,Pur的作用优于Hup-A,联合用药(Hup-A 200 μg/kg+Pur 60 mg/kg)作用最强;各用药都可降低大脑皮层细胞凋亡率,升高大脑皮层某些单胺类递质水平,其中,联合用药的大脑皮层细胞凋亡率低于Hup-A,大脑皮层高香草酸水平高于Pur

Results The total effective rates of treatment group and control group were 82.9% and 63.3% respectively, with a significant difference between them (P.05); the hydroperitoneum extinction in treatment group is earlier than that in control group (P.05); the levels of serum ALT, T-BIL Alb and A/G had significant differences between the two groups.

结果 治疗临床疗效总有效率为82.9%,对照为63.3%(P.05);2治疗后腹水消退时间比较,治疗优于对照(P.05);2治疗后血清ALT、TBIL、Alb以及A/G值比较,治疗优于对照(P.05, P.01);1年后腹水复发率治疗为22.8%,对照为56.6%,治疗优于对照(P.01)。

Results: At five weeks, the radiographically and manually determined fusion rate for autologous bone graft was 50%(4/8animals), compared with 100% and 50%(8/8 and 4/8 anilmals) for groups in which fusions were done by using allograft and xenograft augumented with rhBMP-2. Xenograft+HA used in posterolateral intertransverse arthrodesis yielded a fusion rate of 0%(0/8 animals).

结果:手动试验示:1、自体髂骨即对照融合率为50%:2、各实验融合率分别为:同种冷冻干燥异体骨+rhBMP-2为100%;异种冷冻干燥骨+rhBMP-2为50%;异种冷冻干燥骨+HA为0%。X软片示:1、自体髂骨即对照X线评分均值为2.75分,融合率为62.5%;2、各实验结果分别为:同种冷冻干燥异体骨+rhBMP-2X线评分均值为4分,融合率为100%:异种冷冻干燥骨+rhBMP-2X线评分均值为2.62分,融合率为62.5%。

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