组

It means that decreased expression of Adipo R2, Krs, TNF-αmight be relateted to the action of lipid metabolism by EA treatment, and the down-regulation of Utr gene expression was might be involved in cell damage after simvastatin treatment. To confirm the effect of simvastatin on the changes of gene expression and the state of cell growth, C2C12 was treated in 0, 1, 3, 10, 30 and 100μmol/L of simvastatin.

在正常组、高脂组、辛伐他汀组、针治组、针预组、针非组6个组中，AdipoR2和Krs在针治组表达量最低，TNF-α在针非组中最低，Utr在他汀组中最低，与差异基因的筛选结果一致；同时也说明AdipoR2、Krs、TNF-α在针刺组中下调表达可能与其降脂作用有关；而Utr基因在他汀组中表达减少，可能与该药对肝细胞的损伤作用有关。

No significant difference indrinking capacity was found between HS and FD and its non-early satiety subgroup.Ⅱ. Pathological findings in FD fundic mucosa and its relationship with WLT.(1) significant difference was found in 5-HT positive cell counting between early satietyand non-early satiety subgroups(P.05). but no difference in mast cell counting, activity,and H.

6HS组与FD组及FD的早饱和非早饱亚组在半排时间、排出量和排出率存在显著差异(P.05)，HS组的半排时间、排出量和排出率显著低于FD组及FD早饱和非早饱亚组：HS组与FD的早饱亚组之间的阈值饮水量和饱足饮水量存在显著差异(P.05)，HS组饮水量显著高于早饱组：HS组与FD组及HS组与FD的非早饱亚组之间的阈值饮水量和饱足饮水量均不存在显著差异。

Methods: animals were randomly classified into 5 groups: group a, gaster poured by physiological solution; group b, gaster poured by ethanol; group c, gaster poured by ethanol after hypophrenic vagectomy; group d, gaster poured by ethanol after big small splanchnicotomy; group e, gaster poured by ethanol after hypophrenic vagotomy and big small splanchnicotomy.the distribution of c fos positive neuron in central nervous system was displayed and immunohistochemical staining method was used.

取30只成年雄性wistar大鼠，随机分为5组，每组6只。a组：单纯生理盐水灌胃组；b组：单纯乙醇灌胃组；c组：膈下迷走神经切断乙醇灌胃组；d组：内脏大、小神经切断乙醇灌胃组；e组：膈下迷走神经和内脏大、小神经均切断乙醇灌胃组。运用免疫组化技术显示fos阳性神经元在大鼠中枢神经系统中的分布。

Simulate the condition of temperature and energy control in percutanous MWA for HCC to ablate SMMC-7721 cell strain suspension.There were five experiment groups:A.control group,B.45℃10 min group,C.50℃3min group,D.54℃3min group and E.60℃instant group.Detection for apoptosis was performed at 30 minutes and 2 hours after ablation.2.Thirty-two C57 mice were undertaken hypodermic inoculation of Hepal-6 tumor piece at right groin as ablation tumor and at left groin as observation tumor one week later.The mice with double tumors were separated into four groups:A.control group with no treatment,B.MWA group treated with MWA only,C.mDC group treated with MWA and subcutaneous injection of mature dendritic cells sensitized by neoantigen of Hepal-6 cell strain,D.iDC group treated with MWA and peritumoral injection of immature dendritic cells without neoantigen sensitization.

模拟肝癌微波治疗中常用消融区边缘的温控和能量条件，消融人肝细胞癌株SMMC-7721细胞悬液，分为五组：A、对照组，B、45℃10min组，C、50℃3min组，D、54℃3min组，E、60℃即刻组，消融后30分钟及2小时进行凋亡检测。2.32只C57小鼠左侧腹股沟皮下接种Hepa1-6鼠肝癌细胞瘤块作为消融瘤，间隔1周，右侧腹股沟皮下接种瘤块作为观察瘤，成瘤后分为四组：A组，对照组，不行治疗；B组，微波组，仅微波消融小鼠消融瘤；C组，成熟DC组，微波消融小鼠消融瘤，并于小鼠双侧腋下注射肿瘤抗原致敏的树突状细胞；D组，未成熟DC组，微波消融小鼠消融瘤，并于消融瘤周注射未成熟树突状细胞。

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周，炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节，另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg，20分钟后踝关节照光，激光波长627.sum，功率密度 100mwcm'，照射时间1000秒，能量密度100）／。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径，治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后，治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗，随机治疗一侧膝关节，另一侧作自身对照。兔耳静脉注入I'arrainrelomg／Kg，20分钟后，膝关节用金蒸气激光照射，激光能量密度100）儿旷。24 ／J'时后取膝关节滑膜作病理检查，并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果： 1。模型观察：CIA大鼠炎症高峰期滑膜下炎细胞浸润明显，滑膜细胞明显增殖，炎症达到高峰后二周，血管缀形成，并侵蚀和破坏软骨和骨， CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生，滑膜下炎细胞浸润，也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明，各组织对HMME 的吸收速度和吸收量不同，荧光值一时间曲线不同，滑膜组织比皮肤和软骨对 HMME的吸收多，在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明：PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性，但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好，统计学检验差异有显著性。。3＿军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少，图像分析结果表明面密度（阳性染色的面积总和与统计视野面积的比值）治疗组小于对照组，统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多，图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组，统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小，与对照组相比，统计学检验差异有显著性。结论：二。CIA、AIA的病理改变类似人类RA，可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同，滑膜组织比皮。

Pathological observations showed that the bone marrow and spleen of non-treated IMAA mice demonstrated typical aplastic anemia pattern, including bone marrow failure, marked splenatrophy, but the bone marrow and lymphoid tissues in the IMAA mice of irradiation-treated group were recovered to normal at day 28 after treatment.

结果表明：①IMAA组小鼠活存率为12.5%，平均存活时间为27.4 13.4天，照射治疗组活存率为100%，平均存活时间60天以上，单纯照射组无死亡；②外周血白细胞数：IMAA组呈进行性下降，直至死亡，照射治疗组第10天与免疫再障组相似，以后开始缓慢回升，基本上达到治疗前水平；③红细胞比容：未治疗IMAA组于14天后呈进行性降低，至第35天比实验前减低2/3，照射治疗组与单纯照射组一样，第14天有轻度减低随后升高，至35天接近正常；④骨髓有核细胞数：IMAA组呈进行性减低，无恢复趋势，照射治疗组于一过性减低后迅速增加，第28天达到正常水平；⑤骨髓和淋巴组织病理形态观察：IMAA组小鼠呈典型再生障碍性贫血病理改变，骨髓造血衰竭，脾脏明显萎缩，而照射治疗组于第28天骨髓和脾淋巴脏组织基本上恢复正常。

The expressions of VEGF mRNA in renal cortex in B and group C increased greatly compared with A group at 8th week(P.01), And the expression was decreased more in C group than that in B group at the 8th week;④The light microscopes results showed that no pathological changes in group A; pathological changes were much obvious in group B:glomerular capillary lumen tumbling,lumens blocked,mesangial region widened,basal lamina thicking,mesenterium base inceased,the volume of glomerulus become large,cell population increased,renal tubule vacuolization, renal interstitium was infiltrated by lots of lymphocyte and mononuclear macrophage; pathological changes in group C was light,only see glomerular capillary lumen lightly stegnosis,few lymphocyte infiltrating.

免疫组化结果显示第8周B组大鼠肾皮质VEGF蛋白含量较A组显著增加(P.01)，C组VEGF含量较B组有明显减少(P.01)，C组较A组表达量仍然增加(P.01)；③第8周B组肾皮质VEGF mRNA表达较A组有明显上调(P.01)，与B组相比，辛伐他汀可以明显减少C组肾皮质VEGF mRNA表达；④光学显微镜下A组肾小球毛细血管腔均匀一致，无狭窄，肾小管-间质无炎症细胞浸润。B组则病变较明显：大鼠肾小球毛细血管袢塌陷，管腔闭塞，系膜区增宽，基膜增厚和系膜基质增多，肾小球体积增大，出现玻璃样变；肾小管尤其是近区小管肿胀、变性、空泡形成，肾间质可见大量淋巴细胞和单核巨噬细胞浸润。C组病变较轻，可见肾小球毛细血管管腔轻度狭窄，肾小管-间质见少量淋巴细胞浸润。

There were no significant differences for the firing rates in the site of contralateral TNC neurons among during pre-CSD,CSD,and post-CSD (P＞0.05).For flunarizine group,the firing rates in the site of ipsilateral TNC neurons during pre-CSD were higher as compared with during CSD(P＜0.05).2.1 There were statistical differences on palasma levels of CGRP and SP among the three groups(P＜0.05).The levels of CGRP and SP in CSD group were higher than control group(P＜0.05).No significant differences on the levels of CGRP and SP in ipsilateral trigeminal ganglia were found among the three groups(P＞0.05).2 The number of neurons with positive CGRP and SP immunoreactivity was statistically different in right-sided trigeminal ganglia among the three groups (P＜0.05).The number in fight-sided trigeminal ganglia in CSD group was higher as compared with control group(P＜0.05).The number in right-sided trigeminal ganglia was statistically higher than that in left-sided trigeminal ganglion in CSD group(P＜0.05).3.1 Altered ReHo in ipsilateral pons and other brain regions response to pain such as basal nuclei,thalamus,cingulated gyms and prefrontal cortex was detected during the acute spontaneous attack as compared with during headache remission(P＜0.05,corrected by Monte Carlo simulation). 2 Positive functional connectivity was detected between ipsilateral pons and other brain regions related to pain within pain state and within non-pain state (P＜0.05,corrected by false discovery rate,FDR).Increased functional correlation between ipsilateral pons and other pain-related brain regions such as ipsilateral prefrontal cortex and contralateral subcallosal gyrus was detected during the acute spontaneous attack as compared with during headache remission(P＜0.05,corrected by Monte Carlo simulation).

结果1。对照组未发现CSD；同侧TNC放电频率，CSD中＞CSD后＞CSD前P＜0.05对侧TNC放电频率，CSD前、中、后无统计学差异(P＞0.05氟桂利嗪组同侧TNC放电频率，CSD前＞CSD中(P＜0.05)，CSD前与CSD后及CSD中与CSD后之间无统计学差异(P＞0.05)。2.1关于放免测定，各组血浆CGRP、SP水平有统计学差异(P＜0.05)，CSD组高于对照组(P＜0.05)，CSD组与氟桂利嗪组、对照组与氟桂利嗪组之间均无统计学差异P＞0.05各组之间同侧三叉神经节中CGRP、SP水平未见变化(P＞0.05.2关于免疫组化研究，右侧三叉神经节CGRP、SP免疫阳性细胞数三组之间有统计学差异(P＜0.05)，多重两两比较结果CSD组大于对照组(P＜0.05)，CSD组与氟桂利嗪组之间、对照组与氟桂利嗪组之间无统计学差异P＞0.05左侧三叉神经节CGRP、SP免疫阳性细胞数三组之间无统计学差异(P＞0.05CSD组中右侧三叉神经节CGRP、SP免疫反应阳性细胞数大于左侧(P＜0.05)。3.1局部一致性分析发现两组患者头痛疼痛状态较非疼痛状态脑活动发生变化的脑区有同侧脑桥以及其他疼痛相关脑区如基底节区、丘脑、扣带回、前额叶皮层等(P＜0.05，蒙特卡罗模拟校正)。2功能连接分析发现疼痛状态与非疼痛状态下主要疼痛相关脑区均与同侧脑桥有功能联系P＜0.05，false discovery rate，FDR校正疼痛状态与非疼痛状态比较，同侧前额叶皮层、对侧胼胝下回等疼痛相关脑区与同侧脑桥之间功能联系增强(P＜0.05，蒙特卡罗模拟校正。

So we selected thevinblastine optimum parameters were 0.1μg/ml and 8 hour cultured duration.The fourth, there were significantly differences percent between 10 hourgroups and other three groups in 0.1μg/ml groups; and there were no significantlydifferences between 8 hour groups and 10 hour groups, but they had significantlydifferences among other two groups in 0.3μg/ml groups; and there weresignificantly differences between 10 hour groups and other three hour groups in0.5μg/ml groups; and there were significantly differences between 10 hour groupsand other three groups in 0.7μg/ml groups on the metaphases (P＜0.05), in theexperiment of the effect on the mouse 8- cell embryo stage single blastomere ofvinblastine with different concentrations and duration, by x~2 statistical analysis.

根据实验的实际情况，适宜制备小鼠4-细胞期胚胎单卵裂球染色体标本的长春花碱的处理浓度和时间是0.1μg／ml和8小时。4、在确定长春花碱不同处理浓度和时间对小鼠8-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，阻断培养0.1μg／ml浓度组中10小时组与其他三组差异显著；阻断培养0.3μg／ml浓度组中8小时组和10小时组间差异不显著，但与其他两组差异显著；阻断培养0.5μg／ml浓度组中10小时组与其他三组差异显著；阻断培养0.7μg／ml浓度组中10小时组与其他三组差异显著(P＜0.05)。

Results: The age of onset, the proportion of smoke, the proportion of cough, expectoration, polypnea, cyanosis, rhonchi and moist rale in two lungs were higher in OS patients than OSAS group, the level of body mass index, neck circumference, the proportion of polypnea, nighttime snore, nighttime apnea, daytime hypersomnolence were higher in OS patients than COPD group, the proportion of heart、pulmonary、kidney、 encephalopathy were higher in OS patients than OSAS group and COPD group, FVC, FEV1, FEV1/FVC, MVV, PaO2, SaO2 were lower in OS patients than OSAS group, FRC, PaCO2 were higher in OS patients than OSAS group, AHI was inversely related to the FEV1, FVC, MVV in Group OS, LSaO2 in Group OS was inversely related to the AHI.

结果：OS组的发病年龄、吸烟者比例、咳嗽咳痰、气促、紫绀、肺罗音比例均高于OSAS组，BMI、NC、夜间打鼾、夜间呼吸暂停、白天嗜睡的比例均高于COPD组；OS组合并心、肺、肾、脑等疾病的比例均高于OSAS组及COPD组；OS组的FVC、FEV1、FEV1/FVC、MVV、PaO2、SaO2的值均低于OSAS组，FRC、PaCO2均高于OSAS组；OS组AHI与FEV1、FVC、MVV呈负相关，LSaO2与AHI呈负相关，LSaO2与FEV1、FVC、MVV呈正相关；OS组的最长呼吸暂停时间、S1+2、NREM均高于OSAS组，而LSaO2、REM均低于OSAS组，AHI、最长呼吸暂停时间、S1+2均高于COPD组，LSaO2、S3+4均低于COPD组。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。