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Eighty SD rats were randomly divided into four groups: acute spinal cord injury groups ( SCI groups, n=20);administration of Ulinastatin (U groups, n=20), use Ulinastatin after SCI at half an- and 4- and 8- and 24- and 48- hour.;administration of Methylprednisolone (M groups, n=20), use Ulinastatin after SCI at half an- and 4- and 8- and 24- and 48- hour; administration of Ulinastatin and Methylprednisolone(M+U groups, n=20), use Ulinastatin and Methylprednisolone after SCI at half an- and 4- and 8- and 24- and 48- hour;Allen's weight drop model of acute spinal cord injury was prepared.

本课题利用Allen重物打击模型,选用SD大鼠80只,随机分为四:脊髓损伤(SCI,n=20);使用乌司他丁(乌,n=20);使用甲基强的松龙(甲,n=20);乌司他丁+甲基强的松龙联合用药(乌+甲,n=20),所有给药在脊髓损伤后(半小时、4小时、8小时、24小时、48小时)给药。

The expression of basic fibroblast growth factor in aneurismal wall was determined by immunobistochemical analysis. Results Two weeks after the beginning of the experiment, there was no difference in size of aneurysm among the 3 groups.

结果 各动脉瘤的大小在2周时无明显差异,8周时A明显大于B和C(P.01),B和C差异不显著。2周时,各bFGF均呈轻度表达,8周时AbFGF表达强度明显高于B和C

Results By incubating cardiomyocytes with bFGF, ERKs was activated and the cellular viability 〔(70.0±4.6)%in bFGF 10 ng group vs.(53.0±4.5)%in H/R group, P<0.01) and the ATP content 〔(23.1±2.3) nmol/106 cells in bFGF 10 ng group vs.(12.3±2.1) nmol/106 cells in H/R group, P<0.01〕 were increased, also the LDH leakage 〔(257.3±51.0) U/L in bFGF 10 ng group vs.(372.5±69.2) U/L in H/R group, P<0.05〕, was decreased, then attenuation of H/R injury to cardiomyocytes was observed. PD098059, an inhibitor of upstream kinase of ERKs completely abolished the protective effects 〔viability (57.0±5.8)%,ATP content (15.1±2.6) nmol/106 cells,LDH activity in medium (325.5±59.0)U/L in PD098059 group vs. bFGF 10 ng group, P<0.01, respectively〕.

结果 bFGF激活ERKs,并呈剂量依赖性减轻H/R所致心肌细胞损伤,表现为细胞存活率〔bFGF 10 ng(70.0±4.6)%,H/R(53.0±4.5)%,P<0.01〕和细胞内ATP含量〔bFGF 10 ng(23.1±2.3)nmol/106细胞,H/R(12.3±2.1)nmol/106细胞,P<0.01〕升高,细胞浆酶LDH漏出〔bFGF 10 ng(257.3±51.0)U/L,H/R(372.5±69.2)U/L,P<0.05〕减少;ERKs上游激酶抑制剂PD098059完全消除上述保护作用〔PD098059细胞存活率(57.0±5.8)%,ATP含量(15.1±2.6)nmol/106细胞,培养液中LDH活性(325.5±59.0)U/L,与bFGF 10 ng比较均为P<0.01〕。

RESULTS: Compared with acupuncture and Chinese herb used separately, acupuncture and herb combination significantly decreased the level of reduced viscosity in whole blood, plasma viscosity, blood sedimentation and erythroagglutination index..Significant increases of TMPH in the heart, liver and hind paw tissue except kidney were observed after the combined treatment, which also raise cAMP level in heart and liver.

结果:针药大鼠的全血还原粘度、血浆粘度、血沉、红细胞聚集指数均较单纯针刺、单纯药物显著降低;单位时间内针药大鼠体内(心脏、肝脏、病灶局部织)盐酸川芎嗪含量明显高于单纯药物,肾脏中却无明显增高;同时针药大鼠心脏、肝脏中的cAMP含量明显高于单纯针刺和单纯药物

Results: by the end of 4 weeks, there was significant difference in efficacy between A、C groups and B groups(P<0.05),by the end of 6 weeks, there was significant difference in efficacy between c group and A、B groups ,there was no significant difference in efficacy between A and B group(P>0.05),and the doses of imipramine and the TESS scores of C group were significantly lower than that of B group.

结果:三病人在治疗后4周时,A、C两减分与B减分差异有显著性(P<0.05);治疗6周时A、B两差异无显著性(P>0.05),C效果显著优于A、B两(P<0.01),且C丙咪嗪剂量和TESS评分明显低于B

SEM examination showed that the experimental group has regular spongiest structure,complete bone lacuna structure,partial broken os-teone and visible bone canaliculus, while the sham control group has disorderly structure, broken osrteone, little bone lacuna and total invisibility of bone canaliculus.

结果 X线片示A右侧股骨头骨密度增高,透光区较小,B骨密度减低,有大小不等透光区;ECT扫描示A静态放射性分布中等浓聚,而B静态图像放射性分布呈炸面包圈样改变,欠均匀;织学检查显示A骨空陷窝率、血管数、骨髓腔内脂肪细胞直径较B明显改善(P<0.01);扫描电镜检查显示A松质骨结构较规则,骨陷窝结构完整,部分骨板断裂,骨小管可见。B结构紊乱不清,骨板断裂,骨陷窝少,骨小管消失。

Results The incidence of postcoital vaginal bleeding is lower in postmemoposal wemen than premenopausal wemen, the stages ,the incidences of pelvic lymph nodes metastasis, the results of the Pap smear cytology tests and HPV tests are not significantly different in two groups.

结果:绝经与未绝经比较,绝经阴道接触性出血较未绝经发生率低(P〈0.01),两临床分期、淋巴结转移率、宫颈涂片细胞学检查阳性率及HPV检测阳性率无显著差异(P〉0.05),绝经官颈腺癌发生率占5.2%,未绝经占28.8%(65/226),两中腺癌发生率差异显著(P〈0.01)。

Results: the incidence of postcoital vaginal bleeding was lower in postcoital wemen than that in remenopausal wemen, the stages, the incidences of pelvic lymph nodes metastasis, the results of the pap smear cytology tests and hpv tests were not significantly different in two groups. the incidence of cervical adeocarcinomai was lower in postmenopausal wemen than that in prmenoposal wemen. conclusion: the cervical cancer screening is necessary in postmenopausal wemen and they are helpful for preventing the development of cervical cancer.

结果 绝经与未绝经比较,绝经阴道接触性出血较未绝经发生率低(p<0.01),两临床分期、淋巴结转移率、宫颈涂片及细胞学检查阳性率差异无统计学意义(p>0.05),绝经宫颈腺癌发生率占4.2%,未绝经占28.8%,两中腺癌发生率差异有统计学意义(p<0.01 结论绝经后妇女宫颈癌发病期别通常较晚,绝经后宫颈病变筛查是必要的,定期妇科检查、宫颈涂片细胞学检查可帮助绝经后妇女预防宫颈癌。

It establishes guidance group for subject competition, speech group, training group of scientific innovation, aeromodelling group, sound, figure and beauty training group, training group of broadcast and host, media interview group of television station on campus, training group of information technology,"Prospect" newspaper office, editorial unit of League Sound, dancing group, choir and wind band which largely enrich the life of students.

开设了学科竞赛辅导、演讲、科技创新培训、航模、音体美培训、播音与节目主持人培训、校园电视台新闻采访、信息技术培训、&展望&报社、团委之声编辑部、舞蹈、合唱团、管乐队,大大丰富了学生的生活。

CGRP content in myocardium gradually enhanced from normal group to controls group, and model group's was the highest. We found immunopositive neurons and nerve fibers of nNOS and CGRP by immunochemistry in normal myocardium. They were mainly round-shaped and oval-shaped, also tadpole-shaped、 shuttle-shaped、triangle-shaped、ring-shaped and irregular-shaped. Nv of neurons and nerve fibers in atria were more than in ventricle in some degree; Those in right part of heart were more than in left. Morphology of CGRP neurons changed in model group, the number of ring-shaped neurons increasing, while the round-shaped and oval-shaped decreasing, and Nv and Sv of model group were markedly less than normal. Expression of nNOS mRNA was overly higher than normal group, but CGRP was opposite.

心肌织CGRP的含量由正常、实验对照至CHF逐渐升高,差异显著;免疫化研究表明心肌织中存在nNOS和CGRP免疫阳性神经细胞及纤维,细胞形态多样,正常多以圆形和椭圆形为主,还有梭形、蝌蚪形、三角形、指环形及不规则形,细胞密度心房高于心室,右心高于左心;CHFCGRP神经细胞形态有一定改变,淡染的指环形细胞增多,而深染圆形或椭圆形细胞减少,其面、数密度均明显低于正常,差异显著;用RT-PCR测得CHFnNOS mRNA极度表达,显著高于正常,而CGRP表达明显低于正常

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