组

After the operation, the Didangtang group is treated with Didangtang gastric clysis; the Danshen group was treated with Danshen gastric clysis; while the model group and the normal control group were both treated with physiological brine gastric clysis.

抵当汤组动物和丹参组动物的肝重与模型组动物比较均有显著性差异(抵当汤组P＜0.05，丹参组P＜0.01)；二者的瘤结节数目与模型组比较亦有显著性差异(抵当汤组P＜0.05，丹参组P＜0.01)。

Six weeks after drug intervention,we found,in ADM+Res group,the mean tumor volume of MCF-7/ADM cells implanted tumor was 1.203±0.338 cm~3 with statistical significance to compare with other groups(P＜0.05),decreased about 50.58%compared with the mean tumor volume of ADM alone group1.979±0.307 cm~3the result of the mean tumor weigh was alike to the mean tumor volume; compared with the control,Res could relieve weigh loss(P＜0.05,and the toxicity of treatment was not obvious;Microscopic examination showed that there was massive area of cytoclasis and apoptosis in the tumor tissue of the combination group comparing with other groups.

实验结果提示用药6W后，ADM+Res组移植瘤体积(1.203±0.338)cm~3明显小于其余各组，抑瘤率为50.58%，与单用ADM组(1.979±0.307)cm~3相比有差异P＜0.05肿瘤重量的结果与瘤体结果类似；未观察到用药后的毒副作用；经不同处理后，单独注射Res组小鼠体重较生理盐水组及ADM组有所增加(P＜0.05，而ADM+Res组治疗前后体重无差异P＞0.05HE结果显示与其他组相比，ADM+Res组肿瘤组织中出现大片坏死区域。

The biomechanical tests showed that two kinds of artificial bones had not significant difference on compressive strength and Young\'s modulus(P＞0.05),while the flexural strength of nano-nacre artificial bone was less than the control group(P＜0.05).3.The results of CCK-8 showed that the difference were not significant in each group,the proliferation of osteoblast reached the peak at the 5th day;7 days after being co-cultured,the total protein content of study group was higher than control group and blank group(P＜0.05),while the difference between control group and blank group was not significantP＞0.05The difference of alkaline phosphatase activities among three groups was not significant(P＞0.05The SEM view showed that osteoblast attached and grew well in two kinds of artificial bone.4.X-ray photography showed that two kinds of powder started to degrade in 2 weeks;this phenomenon became more appear in 4 weeks,nano-nacre powder degraded faster than micron-nacre powder,while the hole shadow was easy to be found;in 8 weeks,all the femoral holes recovered and returned to normal bone mineral density in all groups.Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone grew fastest around the bone defect area in study group,while most slowly in blank groupP＜0.05 SEM(scanning electron microscope observation showed that nano-nacre powder degraded more quickly.The same result can be found through the demineralized sections morphometric analysis,and both of the composite artificial bones made from those two kinds of nacre powder had the good connection with the adjacent tissue in rats body without apparent inflammatory response.5.X-ray photography showed that rabbit\'s bone defects healed faster in study group since NNAB implanted than in control group since MNAB implanted.At 24 weeks after operation,bone density in radial defects had nearly accessed to the normal area,while lower in control group,and turned up nonunion in blank group;The checking of BMD showed that results in study group were higher than those in control group at 8,16 and 24 week(P＜0.05), and the difference between the BMD values in study group at 24 week and those in blank group was not significant(P＞0.05).The gross specimens showed satisfactory histocompatibility both in study group and in control group,with bone tissue growing from two sides into the center of implanted materials; Normal slices in HE stain and hard tissue grinding slices in Stevenel\'s blue/Van Geison\'s picro-fuchsin stain showed that the bone growth tendency was better in study group than that in control group,and the medullary cavity had been penetrated to the implanted materials in study group at 24 week;Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone in both groups grew fastest 8 weeks after surgery,while slow down at 16 week.

纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨分别与成骨细胞共培养后，其各时间点CCK-8法检测值与空白对照无显著差异(P＞0.05)，成骨细胞均在第5天达到增殖高峰期；培养7天后，实验组细胞蛋白含量高于对照组及空白组(P＜0.05)，后两者之间则无显著差异P＞0.05碱性磷酸酶活性在三组间均无显著差异(P＞0.05电镜下可见成骨细胞在两种人工骨上都有良好生长贴附能力。4.X-ray显示两种粉体在大鼠股骨骨洞植入第2周时都开始出现了降解，第4周时更为明显，纳米珍珠层粉较之微米珍珠层粉降解更快，而空白对照组骨洞阴影仍可见，至8周时，则所有组骨洞均己闭合修复，X-ray下已不可见原钻孔痕迹，恢复正常骨质密度；硬组织磨片四环素荧光双标记结果显示纳米珍珠层粉植入组较其余两组在骨缺损区周围新骨生长速度更快，空白组速度最慢P＜0.05电镜观察及常规脱钙切片亦可见到纳米粉体降解较快；由以上两种原材料制得的纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨在大鼠体内均与周围组织结合良好，无明显炎症反应。5.X-ray显示纳米珍珠层/消旋聚乳酸复合人工骨植入兔桡骨缺损区后其骨愈合速度较对照组微米珍珠层/消旋聚乳酸复合人工骨植入的快，至植入术后24周，实验组骨缺损区接近正常骨密度，对照组骨缺损区密度较低，空白组则呈现骨不连状态；骨密度测量结果显示术后8周、16周、24周实验组的骨密度值高于对照组(P＜0.05，24周实验组的骨密度值与术前所测得的正常值无显著性差异P＞0.05动物取材大体所见均显示组织相容性良好，骨组织逐渐由植入材料两端向中央生长；常规切片HE染色及硬组织磨片Stevenel\'s blue/Van Geison\'s picro-fuchsin联合染色均可见实验组骨缺损区长势优于对照组，至术后24周，实验组骨髓腔与材料已呈相交通状；硬组织磨片荧光显微镜下观察，两组材料在术后8周处于骨生长最快速时期，16周时速度开始减慢，术后4、8、16周时实验组的新骨生长速度均较对照组的快

Methods: Thirty male SpragueDawley rats were randomly divided into 5 groups, group A 3 days after L5 VRT, group B 7 days, group C 14 days, group D 21 days and group E of sham operation, whose left spine cords were exposed by hemilaminectomy. Rats in each group were respectively perfused with paraformaldehyde and materials were taken from L5 dosal root ganglions and the changes of expression of P2X3 receptor were observed with immunofluorescence staining.

雄性SD大鼠30只，随机分为5组（n=6），A组（L5 VRT术后3 d）、B组（L5 VRT术后7 d）、C组（L5 VRT术后14 d）、D组（L5 VRT术后21 d）模型组和E组，E组仅行半椎板切除术暴露左侧脊髓，分别在各自的时间点灌注取材L5背根神经节，用免疫荧光染色方法观察P2X3受体表达的变化。

And more, ERK contents between group A and B, group C and D were not dramatically different. The pathological lesions indicated that intestinal tissue necrosis was severe in group C and D, which were graded 3 points, but obviously lessened in group A and B, which was graded 1 point, with ITF interfering. Conclusion: Intestinal inflammation was ameliorated after ITF was injected hypodermically or intraperitoneally.

结果：A、B组组织匀浆中ERK的面密度值各为87.68±19.24、82.65±21.35，较C、D组(30.23±7.79、30.74±8.19)明显升高(P＜0.01)，C、D组与E组(5.41±1.46)比较也有升高(P＜0.05)；并且随著ERK的激活伴随著明显的核转位。A、B组间及C、D组间ERK含量无显著差异(P＞0.05)；病理切片显示C、D组HE染色切片见肠壁损伤轻重不一，可见全肠粘膜绒毛坏死，病理评分的中位积分为3分；A、B组肠上皮细胞少量脱落，顶端绒毛坏死，病理评分的中位积分为1分。

Methods: the present study improved isolation of LDL, undertook one-step of density gradient centrifugation, generate E-LDL by treatment LDL with multiple enzymes, direct fluorescent label CRP and LDL, and investigate whether CRP play as intermedial mediator in atherosclerosis. Experiment consists of 8 groups: control group, N-LDL group, ox-LDL group, E-LDL group, CRP+N-LDL group, CRP+ox-LDL group, CRP+E-LDL group.

本研究参考文献改进LDL提取方法，通过一次性密度梯度超速离心，分离LDL，并且通过复合酶处理以及铜离子氧化法分别生成E-LDL和ox-LDL，并直接对CRP及LDL进行荧光标记，通过与内皮细胞共孵育，利用荧光显微镜检测CRP及LDL与内皮细胞结合，实验分为8组：空白对照组、CRP组、N-LDL组、ox-LDL组、E-LDL组、CRP+N-LDL组、CRP+ox-LDL组、CRP+E-LDL组。

Gln group received pure Gln through a jejunostomy tube, IN group received indomethacin, G+I group received Gln and indomethacin.

材料与方法：将SD大鼠随机分成四组：谷氨酰胺组（Gln组，n=34）；吲哚美辛组（IN组，n=34）；谷氨酰胺+吲哚美辛组（G+I组，n=34）和SAP组（n=34）。

At 4-week, GAG content in JG as significantly lower than that in RG and CG, while dead cell ratio in RG significantly higher than those in JG and CG. Mankin scores in RG and JG ere significantly higher than that in CG. At 8-week, GAG content in RG as significantly lower than that in CG, while dead cell ratio and Mankin scores ere significantly higher than those in CG.

结果 显微观察显示两组训练动物在训练4周时便开始有膝关节软骨过劳性损伤早期改变，8周时损伤改变进一步加重。4周时，蹦跳组GAG含量明显低于对照组及跑步组，跑步组死亡细胞率明显高于对照组以及蹦跳组，跑步组和蹦跳组Mankin评分明显高于对照组；8周时，跑步组GAG含量明显低于对照组，死亡细胞率与Mankin评分明显高于对照组。

Results:The rate of tumor shrinkage and necrosis was 46.8% and 20.7%,while the contrast group was 78.9%,49.2% respectively(P＜0.05).There were significant difference(P＜0.05)for 2year survival rate in singlelesion group,nondistant metastasis group and combinedtherapy group.

结果：单纯TACE组的肿瘤总缩小率和肿瘤完全坏死率分别为46.8%和20.7%，联合治疗组分别为78.9%、49.2%（P均＜0.05），单病灶组、无转移灶组及联合治疗组的2年生存率高于多病灶组、有转移组和单纯TACE组（P均＜0.05）。

However, the Oestradiol level of RSA patients was lower than non-pregnancy women obviously. CD4+CD25+Treg of normal pregnancy women (early pregnancy, mid-pregnancy and late pregnancy) were higher than non-gestation women obviously. CD4+CD25+Treg were at peak in mid-pregnancy. The CD4+CD25+Treg were obviously lower in RSA patients than non-pregnancy women.

早、中、晚孕组外周血CD4+CD25+Treg细胞数均明显高于正常非妊娠组（t值分别为2.22，8.9，5.91，P均＜0.05），中孕组明显高于早孕组（t=6.31， P＜0.05），晚孕组略低于中孕组，但差异无统计学意义；反复自然流产组明显低于正常非妊娠组（t=8.07， P＜0.05）。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。