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Results For callus induction of leaf explants of C. orchioides, dark treatment gave better results compared to light treatment; among the media tested, the suitable phytohormone combinations were 2.0 mg/L 2, 4-D or 6-BA 1.5 mg/L 2, 4-D 2.5 mg/L, and 300 mg/L CH 0.2% AC was good for plantlet regeneration from leaf explants. The callus from leaf explants mainly originated from midrib. The parenchyma cells near epicuticle of midrib firstly were initiated to division. Then the parenchyma cells of vascular bundle sheath and mesophyll cells on each side of vascular bundle were also divided to form callus.

结果对仙茅叶片的愈伤诱导，黑暗的效果好于光照；在所试验的培养基成分中，适宜的激素配比是2.0mg/L2，4-D或6-BA1.5mg/L 2，4-D2.5mg/L，并且附加300mg/L水解酪蛋白和0.2%活性炭，对于仙茅叶片的离体成苗较好；培养后，愈伤组织主要由叶片的中脉产生，位于中脉上表皮内侧的薄壁细胞首先启动分裂，随后维管束鞘薄壁细胞及其外侧的叶肉细胞也启动分裂，参与愈伤组织的形成。

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