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In order to know the nemsticifal activities substance and the mechanisms of Datura metel Linn, total alkaloids from the leaf of Datura metel Linn was extracted and identified, and studied the mechanism of it by determining the activity of Ache and the Ach content of Meloidogyne incognita.

为了解白花曼陀罗的杀线活性物质及其作用机理,对白花曼陀罗叶的总生物碱进行了提取鉴定,并通过测定乙酰胆碱酯酶活性及乙酰胆碱含量研究其作用机理,结果表明:生物碱是白花曼陀罗的重要杀线活性物质,可抑制线虫乙酰胆碱酯酶活性,提高线虫乙酰胆碱含量,直接作用于线虫的神经系统。

Nematicidal Activity to Different Nematodes of Basidiomycetes from Shenyang CHEN Li-jie, ZHANG Guo-dong, DUAN Yu-xi, LIU Bin, WANG Yuan-yuan (Plant Nematology Laboratory, Shenyang Agricultural University, Shenyang 110161, China) Abstract: The 94 kinds wild macro-fungi of Basidiomycetes were collected from the east of Shenyang around mountains and Shenyang Agricultural University, China. The nematicidal activity of extraction for the fungi were tested with 5 speceies of nematode, including Heterodera glycines, Meloidogyne incognita, Ditylenchus destructor, Aphelenchoides basseyi and Caenarhabditis sp..

沈阳地区大型真菌对不同种类线虫的毒力陈立杰,张国栋,段玉玺,刘彬,王媛媛(沈阳农业大学植物保护学院线虫学研究室,沈阳 110161)摘要:从沈阳市东部山区及沈阳农业大学周边环境采集到94种野生大型真菌,并将采集到的大型真菌制成浸提液,以胞囊线虫、根结线虫、腐烂茎线虫、水稻干尖线虫和小杆线虫为靶标,初步测定了大型野生真菌对5种不同种类线虫的活性作用。

According to the predacity of the nematode-trapping fungi and the growth habit of pine wood nematode Bursaphelenchus xylophilus, this paper tested the B. xylophilus that cultured by the fixed time and quantities. Trying to find out the quantificational test method of trapping rate, by the change of the quantities of B.

本文依据捕食线虫真菌的捕食特性以及松材线虫的生长习性,对松材线虫进行了定时、定量的测定试验,试图通过培养后线虫的种群数量变化,来摸索定量测定捕食率的方法。

There were no significant differences in soil bacterial and fungal biomass, total nematode density and diversity, herbivore density, bacterivore density and omnivore-predator densities between the trenched and control plots in both mineral and humus soil layers.

切根处理对腐殖质层和矿质土层的细菌和真菌生物量、线虫总密度和多样性、植食性线虫、食细菌线虫和捕食与杂食性线虫的密度均没有显著影响。

According to sequence cluster analysis of the the tested samples, it indicates that the gene distance of RKN-9, RKN-8, RKN-21, RKN-11, RKN-18 , RKN-20, RKN-17, RKN-22, RKN-23, RKN-24 and RKN-25 to Meloidogyne incognita and Meloidogyne arenaria was very close with homology of 100% and 99%, respectively.

根据25个根结线虫种群序列比对分析和聚类分析可知,河北廊坊的番茄根结线虫、安徽宿州的黄瓜和番茄根结线虫、安徽太和的桔梗根结线虫、安徽和县的番茄根结线虫、浙江杭州的黄瓜根结线虫、四川成都的番茄根结线虫、安徽泗县的黄瓜根结线虫和中国农业大学四个不同番茄品种上的根结线虫的序列之间同源性为100%;它们与花生根结线虫和南方根结线虫(M.incognita)的遗传距离最近,序列同源性分别达到100%和99%。

Structure index was significantly (P.05) different among three plant species whereas channel index and enrichment index were not. Plant species not only modified abundance of plant-feeding, omnivorous and predatory nematodes, but also percentage of each trophic group. Bacterivorous and fungivorous nematodes were affected most by the differences in plant species.

研究表明在沙地生态系统中,3种植物根际土壤线虫群落的多样性、丰富度、均匀度和线虫总数虽然都存在一定的差异,但不显著;功能多样性指数中结构指数存在显著差异,而通道指数、富集指数不存在显著差异;3种植物对根际土壤线虫群落中各营养类群线虫的比例有显著影响。

The predominant worm species were Haemonchus contortus, Oesophagostomum columbianum, Bunostomum trigonocephalum, Trichostrongylus sp.

羊胃肠道线虫的优势虫种是捻转血矛线虫、哥伦比亚食道口线虫、羊仰口线虫、毛圆线虫和毛首线虫。

The result indicated the basic culture had poisonous effect to nematodes, and significant virulence to different nematodes was detected, the sequence of virulence to nematodes was Caenarhabditis sp., Heterodera glycines, Meloidogyne hapla, Ditylenchus destructor.

结果表明,该发酵液呈微碱性,不同浓度发酵液对各种线虫均有较好的防治作用,并且毒力作用具有明显差异,对4种线虫的毒力大小顺序分别为小杆线虫、大豆胞囊线虫、根结线虫和腐烂茎线虫。

The dominante race of SCN is still race 3 in Heilongjiang Province. But the SCN race 3 changed into race 4 and 14 in Daqing City and Anda City, indicating some changes happened in Heilongjiang. A distribution map of soybean cyst nematode in Heilongjiang was drawn , which made clear the style and distribution of SCN race in Heilongjiang, and provide the basis for soybean breeding for SCN resistance species and the rational distribution of resistant cultivars.2. Soybean cyst nematode, Heterodera glycines Ichinohe, populations were selected on eight soybean Glycine max L.

大豆胞囊线虫3号生理小种仍然是黑龙江省大豆胞囊线虫的优势小种,大庆市和安达市由原来SCN3号生理小种分别变为4号和14号生理小种,表明黑龙江省SCN生理小种稳中有变;绘制了黑龙江省大豆胞囊线虫分布图,明确了黑龙江省SCN生理小种的类型和分布,为抗线育种及抗线品种合理布局提供依据。2。

Some strains of the nematode-bearing bacteria were bioassayed for their parasitism. All these bacteria can totally not grow or grows badly on the media incorporating the extracts of pine trees, indicating some substances existing in the trees will harmful to the bacterial strains, which is further convinced by preliminary inoculation showing that single bacterial species will not infect Pinus thugbergii. Hence, the growth and reproduction of the nematode-carrying bacteria, which could produce toxic substances causing the death of the tree cells, is closely related to the nematodes, when they first enter to the trees. In the same time, the activity of bacteria improved the circumstances for which nematodes are living, which in turn will beneficial to the growth of nematodes. Thus, there is a complicated interaction among nematodes, bacteria, and pine trees.

对松材线虫携带的细菌的部分菌株进行了寄生性测定,表明这些细菌在添加松树提取液的细菌培养基中不能生长或基本不能生长,这说明松树体内可能存在某些不利于细菌生长的物质,同时初步的接种试验证实,单独的细菌接种不能感染健康的黑松,因此,线虫携带的致病细菌在最初进入树体时,其生长和繁殖与线虫密切相关,结合细菌产生的毒性物质可造成黑松细胞死亡的情况,说明细菌的生长与线虫有关,同时,细菌的活动改变了线虫在松树内的生长环境,使环境变得有利于线虫的生活,因此,线虫、细菌和松树三者存在着复杂的生物关系。

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