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Along with the fluconazole solution density rise,the experimental two kind of strain various glucose density is higher,showe d the glucose consumption are less,takes the logarithmof the medicine de nsity,discovered the logarithm the medicine density and each glucose den sity presents the linear relations;Carries on the analysis comparison to under the fluconazole function two kind of strain linear relations,disc overed the relations of the two strains has the nonuniformity.3 Compare the fluconazole induction reaiatance SC5314 strain and sens itive strain compares,its difference gene expression mainly concentrates in:The code proteinase body and the protein hydroltyic enzyme gene,in the code sugar fat metabolism process is connected the protein gene,the cell cycle correlation gene,the duplication and the translation adjustme nt correlation gene,the stress response correlation gene,the line plast ochondria correlation gene,the cell wall function related gene.4 Candida albicans SC5314 induction resiatance strain was processed b y Xianglian solution,its expression change gene mainly is:Code stress re sponse family protein gene,biomembrane relevant gene,a code proteinase body gene race,code cell cycle related protein gene,duplication and tra nslation adjustment related protein gene.5 The clinical reaiatance strain Candida albicans was processed by Xi anglian solution,its expression change gene mainly is:Codes the hot sho ck protein gene,the serine/threonine protein activating enzyme gene,the proteinase body family gene,the regulation copies and translates the ge ne.

随着氟康唑药液的浓度上升,试验的两种菌株各孔葡萄糖浓度越高,说明葡萄糖消耗越少,经过药物浓度取对数后进行分析,发现取对数后的药物浓度和每孔中葡萄糖浓度者呈现线性关系;对氟康唑作用下的两种菌株的线性关系进行分析比较,发现对两种菌株作用具有不一致性。3氟康唑诱导的耐药SC5314菌株与诱导前的敏感株相比,其差异基因表达主要集中在:编码蛋白酶体及蛋白水解酶的基因,编码糖脂代谢过程中相关蛋白的基因,细胞周期相关基因,转录及翻译调节相关基因,应激反应相关基因,线粒体相关基因,细胞壁功能相关基因。4白念珠菌SC5314诱导耐药株经香莲外洗液作用后,其表达变化的基因主要是:编码应激反应家族蛋白的基因,生物膜相关性基因,编码蛋白酶体基因一族,编码细胞周期相关蛋白基因,转录及翻译调节的相关蛋白基因。5白念珠菌临床耐药菌株经香莲外洗液作用后,其表达变化的基因主要是:编码热休克蛋白基因,丝氨酸/苏氨酸蛋白激酶基因,蛋白酶体家族基因,调控转录及翻译基因。

Results showed that TDI induced toxic action on spermatogenic cell,while the chondriosome was its target within the length and concentrations.

结果表明:在本实验染毒时间和染毒剂量范围内,TD I对雄性的生殖毒性主要表现为对各级生精细胞均有一定的毒性作用,作用的靶点是细胞内的线粒体;TD I通过影响各种酶的活性,干扰了睾丸组织的有氧代谢和无氧供能,抑制了细胞对能量的利用,损伤了各级生精细胞,导致生精上皮不可逆的损害,从而造成了其对雄性生殖系统的损伤;TD I对雄性生殖细胞的核酸代谢以及DNA合成有一定影响。

Because chronic obstructive inflammation of airway severely influences the ventilation and exchange function of COPD patients, The motor ability of patients is limited .Despnoea and fatigue limit patients to sedentary,because of this, skeletal muscle began to analosis,oxygen uptaking capacity of skeletal muscle descends,and concentration of chondriosome degrades.Function limitation of skeletal muscle causes oxygen needs increase,so vicious cycle emerges,and further to restrict patient's respiratory and motor function.

由于气道的慢性炎症性阻塞,严重影响了慢阻肺患者的通气及换气功能,使得患者的运动能力明显受限,因喘息和疲劳而限制了患者的运动能力,使患者出现久坐,由此出现骨骼肌的萎缩,骨骼肌摄氧能力下降,线粒体氧浓度降低,骨骼肌的功能受限又会引起通气量不足,进而引起低氧血症和高碳酸血症,使患者的摄氧需求增加,由此出现恶性循环,进一步制约了患者的呼吸和运动功能。2001年正式提出肺康复作为中重度COPD患者稳定期非药物治疗的首选管理方案。

Beijingensis under three droughttreatments, normal, moderate and severe stresses. The results showed:(1) the canker disease ofboth cultivars was serious gradually with increased drought;(2) the bark tissue cells sufferedplasmolysis, more evident with the severity of drought stress;(3) under the drought andinoculation with B. dothidea, cells of two cultivars damaged at different degree, mailyrepresented in the changes of organelles, such as chloroplast swollen and distorted, number ofmitochondria increased and membrane system indistinct; then organelles suffered furtherdamagement with inoculation time, thinned mitochondrias stroma, decreased cristae, crumpledand partly broken membrane of chloroplasts with stroma exosmosis. At last, the chloroplastspartly disorganized;(4) the hyphae growed mainly intercellular in resistant cultivar and notonly intercellular but also intracellular in susceptible cultivar, which directly caused thenecrosis of cells;(5) under the severe drought, the damage of cells enhanced the infection ofpathogen and drought and pathogen stressed together and promoted the disease development;the damage from pathogen on cells was more serious than that from drought.

结果显示:(1)随着干旱胁迫程度的增加,2种杨树溃疡病害发生渐趋严重;(2)干旱胁迫下,杨树树皮组织细胞发生质壁分离,并随胁迫程度的增加而严重;(3)干旱胁迫下接种病原菌,2种杨树细胞发生不同程度的损伤,主要表现为细胞器发生较大变化,如出现叶绿体肿胀变形、线粒体数量增多,质膜模糊不清等现象;随接种时间的延长,细胞器受到进一步损伤,叶绿体被膜折皱,严重时局部破裂,基质外渗,并部分最终解体;(4)毛白杨中的菌丝主要在细胞间隙中穿行,而北京杨的菌丝除在细胞间隙中生长之外,侵入细胞内部也较多,直接导致细胞的解体;(5)干旱胁迫下细胞的损伤促进了病原菌的侵染,干旱和病原菌的双重胁迫加剧了病害的发生程度,并且病原菌侵染对细胞的破坏程度大于水分胁迫。

To date, only the structures of prokaryotic succinate:ubiquinone oxidoreductases, which share a similar enzymatic function with mitochondrial SQR, have been reported, including QFR from E.coli, QFR from Wolinella succinogenes and SQR from E.coli.

在此之前,只有原核生物的琥珀酸泛醌氧化还原酶的结构得到了解析,这包括大肠杆菌的 QFR , Wolinella succinogenes 细菌中的 QFR 和大肠杆菌的 SQR ,这些蛋白有着与线粒体复合物 II 相类似的酶功能。

Silkies were the mutation type of nomal feather Wugu chicken for it had more haplotypes and shared the major haplotypes with silkies.4. The sub-species of G. g. spadcieus and G. g. jabouillee in China were at the same clade with the G. g. gallus in and around the Thailand area, but were in difference clade with G.

在中国的两个红原鸡亚种的线粒体D环高变区序列与在泰国等地分布的红原鸡亚种的此段序列差异不大,可归为一类,而与爪哇岛上分布的红原鸡亚种的此段序列差异较大。

Among them multi-types of body colour and special types of hosts are the most universal phenomena. In our experiment, methods of microsatellite primer PCR molecular marker and partial gene sequence analysis of mitochondrial DNA COⅠ-Ⅱwere used to analyze genetic diversity of two body colour bio-types (red bio-type and green bio-type) of green peach aphid from different plants and relationship between green peach aphid and host plants by separately selecting individuals of aphid from rape, Chinese cabbage and tobacco. The main results as follows: 1. Selecting An Ruisheng'method of extracting DNA from small sized insect, using this method can extract DNA well and steadily.

本文采用微卫星引物PCR 分子标记和线粒体COⅠ-Ⅱ部分序列分析的方法,分别选取十字花科植物中的甘蓝和油菜,茄科植物中的烟草,对不同寄主上桃蚜的红色和绿色两种体色生物型的遗传多态性及其与寄主植物的关系进行了研究,取得的主要结果如下: 1、选取安瑞生(2002)小型昆虫DNA 提取方法,能很好且比较稳定的提取桃蚜基因组DNA。

In most species they are composed of roughly equal amounts of protein and ribosomal RNA , the ribosome consists of two unequally sized rounded subunits arranged on top of each other like a cottage loaf, eukaryotic cells have larger ribosomes than prokaryotic cells but the ribosomes in mitochondria and chloroplasts are about the same size as prokaryotic ribosomes.

在大多数的物种中,蛋白质的量和 rRNA 量是大体相等的。核糖体包括两个大小不同的亚单位,上部结合形成小屋状结构。真核生物细胞中的核糖体要比原核细胞的核糖体大,但是线粒体和叶绿体中的核糖体与原核细胞的核糖体大小相当。

Gamma interferon-inducible protein 10 induces HeLa cell apoptosis through a p53-dependent pathway initiated by suppression of human papillomavirus type 18 E6 and E7 expression . Mol Cell Biol.

另外,体外研究也发现[10],在CVB3感染后的HeLa细胞中P53的水平明显升高,而P53的上调可使Bax表达增高,从而导致线粒体cyt-c的释放和caspase-3的激活,诱导细胞的凋亡。

PCI2 cells were treated under glucose deprivation as model group. Method Morphological and cytoflowmetric methods (the mitochondrial transmembrane potential; MEF method) were applied to evaluate the protective effect of SAB. The RNA levels of grp75 were measured by RT-PCR and immunocytochemistry were performed to investigate the expression level of PC12 cells under glucose deprivation and treated by SAB.

以PCl2细胞(大鼠肾上腺嗜铬细胞瘤细胞)的无糖培养建立细胞缺糖损伤模型,MTT法检测细胞的存活率;应用流式细胞仪检测亚二倍体细胞的比率及线粒体跨膜电位的改变;应用免疫细胞化学、RT-PCR法研究细胞内应激蛋白grp75的表达变化,激光共聚焦显微镜观察SAB作用下Ca(上标 2+)的变化。

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