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You solubilize the ammonium sulfate pellet containing the mitochondrial proteins and dialyze it overnight against large volumes of buffer ed (pH 7.2) solution.

你把含线粒体蛋白的硫酸铵沉淀溶解,用大体积的缓冲了的(pH7.2)溶液透析过夜。

Here, we examine the phylogenetic relationships of the Clemmys complex of the freshwater turtles using 985bp of the DNA encoding the mitochondrial genes ND4 and adjacent tRNAs gene. These data contain 235 parsimony informative characters that we use to erect hypotheses of relationships for the Geoemydidae and Emydinae turtles.

本文测定了四眼斑水龟和黄喉拟水龟线粒体基因组的ND4基因,用部分ND4基因及相邻tRNA基因共985bp的序列(包含简约信息位点235bp)讨论了它们的系统进化关系。

The phylogenetic relationships of Fagales were investigated by means of molecular systematics, which was based on six DNA sequences from three genomes, including chloroplast DNA trnL-F, matK, rbcL, and atpB, nuclear 18S ribosomal DNA, and mitochondrial gene matR.

本文采用来自三个基因组--叶绿体、线粒体和核基因组的六个DNA序列片段,对壳斗目的系统发育进行了重建,主要探讨了壳斗目八个科的科间系统演化关系及不同基因系统树间存在冲突的原因。

Codon partition schemes were compared in phylogenetic analyses at subfamily level for Gyrinidae and at family level for Hydradephaga based on partial mitochondrial COⅠ gene sequences.

利用PAUP和MrBayes软件,对线粒体COⅠ基因序列3个密码子位置的数据模块分别进行了豉甲科和水生肉食亚目在亚科或科水平上的系统发育学分析,结果表明第二密码子数据模块获得了理想的分析结果。

Results show that these sequences are homological to Brassica napus and Arabidopsis mtDNA genome partially. But they are not homological to CMS gene reported before.

结果表明,所获得的特异片段均与拟南芥和甘蓝型油菜的线粒体基因组序列部分同源,但与已发表的不育基因序列没有同源性。

The result ofpairwise Fst and Neutrality tests revealed significant genetic differentiation amongfour populations,and evolution of mtDNA COI gene incompliance with the Neutralitytheory.The genetic distance was the biggest between HEB and JXL,and was thesmallest between SDT and JXL among the 4 populations.

遗传多样性研究采用了线粒体DNA COI基因序列、RAPD和PCR-RFLP三种分子标记研究大猿叶甲4个地理种群的遗传结构和群体遗传多样性。1、对长约335bp的mtDNA COI片段进行序列分析,在4个群体64个个体中,共发现37个单倍型,总变异位点35个,多态位点29个,含量(63.68%)大于含量(36.32%)。4个地理种群的核苷酸多样性指数分别为,0.082、0.035、0.099、0.186。

In order to analyze proteins which may interact with AIF, interceptive AIF gene (mitochondrial localization sequence, MLS, was deleted) which was amplified from the total mRNA extracted from hepatoma HepG2 cells by reverse transcriptase polymerase chain reaction, was inserted into pcDNA3.1 plasmid.

为了筛选可能与截断型AIF存在相互作用的蛋白质,探讨截断型AIF诱导细胞凋亡的机制,用逆转录PCR扩增剪切掉线粒体定位信号的人AIF基因片段并克隆入pcDNA3.1载体,导入肝癌HepG2细胞。

White fat, or white adipose tissue, is the jiggly stuff that stores spare energy from food. By contrast, brown adipose tissue consumes energy to generate body heat. The tissue teems with mitochondria that metabolize food, hence its color.

白色脂肪组织用来贮存消化吸收而来的多余能量,而褐色脂肪组织则消耗能量来产生热量,它内部充满着大量的线粒体,因此看上去是褐色的。

Results: When the concentrations of artemisinin were 1×10^(-4), 1×10^(-5) and 1×10^(-6)mol/L, the growth of cells was inhibited remarkably in a dose-dependent manner. Fluorescence staining showed obvious apoptosis, such as karyopyknosis and agglutination. RT-PCR assay shows the expression of Caspase-3. Artemisinin could lead to the decrease of mitochondrial cytochrome c concentration, which in turn lead to the increase of cytoplasmic cytochrome c concentration.

结果:青蒿素的浓度为1×10^(-4),1×10^(-5),1×10^(-6)mol/L时,细胞生长受到显著抑制,并呈剂量依赖性;Hoechst33342/PI双荧光染色可观察到明显的核浓缩、凝集等细胞凋亡表现;RT-PCR检测到Caspase-3的表达;Western-blot检测1×10^(-5)mol/L药物处理细胞后线粒体细胞色素C表达水平下调,细胞浆出现明显细胞色素C蛋白条带。

The complete sequences of mitochondrial Cyt b gene in Pagrus pagrus , Sparus macrocephalus , Acanthopagrus latus and Parargyrops edi ta in Zhoushan sea area were amplified, cloned and sequenced.

克隆测序了分布于舟山海域的真鲷、黑鲷、黄鳍棘鲷和二长棘鲷4种鲷科鱼类的线粒体Cyt b 基因1 141 bp的全序列。

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