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Methods Rat calvaria osteoblasts were cultured in the presence of 10 μg/mL-500 μg/mL nHA or nAg-HA/TiO2 for 2 h, 6 h, 8 h, 24 h, 72 h and 120 h. Cell proliferation, vitality and ultrastructure were tested using cell counting chamber, MTT assay, and transmission electron microscope.

将成骨细胞分别在添加了不同浓度(10μg/mL~500μg/mL)的nHA或nAg-HA/TiO2的培养基中培养2、6、8、24、72及120h,通过透射电镜观察、细胞计数及MTT法检测细胞超微结构、细胞增殖及线粒体活性,并计算单位细胞呼吸率。

The purpose of this study was analysis and tracking the heredity stability of Alpaca through amplifying special fragment from mitochondrion genome. A pair of primer was designed according to those sequences of Camelidae animal submitted in GaneBank and a fragment of 1014 bp was cloned.

摘 要:本试验对GaneBank中登录的部分骆驼科动物线粒体12srRNA/tRNA-Val/16rRNA基因序列进行同源性比较,并借助DNAstar软件设计引物,扩增并进行序列分析,旨在揭示其遗传变异的规律。

Results The electron microscopical observation demonstrated that intracellular lanthanum could be found,most of which entered the hepatocytes and its mitochondria. The lanthanum granules were also found to deposite in the zone of tight junctions of bile canaliculi, indicating that the tight junctions had been damaged.

结果 镧示踪标记法显示镧颗粒进入了细胞及线粒体内;毛细胆管紧密连接区也出现了致密的镧沉积,并与肝细胞间隙的镧沉积物相连,表明毛细胆管紧密连接已开放。

The phylogenetic relationship of raptors and nighthawks were estimated by mitochondrial rRNA gene sequences in this study by comparing the partial basepair fragments of 12S rRNA and 16S rRNA genes among 5 species of Falcoiformes, 3 species of Strigiformes and 1 species of Caprimulgiformes sequenced in this experement, together with the homologous segments of mtDNA in other 17 species of birds downloaded from GenBank.

本文测定5种隼形目鸟类、3种鸮形目鸟类和1种夜鹰目鸟类的部分线粒体12S rRNA基因和16S rRNA基因,结合从GenBank下载的17种鸟类同源基因进行系统进化研究。

Reactive oxygen species scavenger N-2-mercaptopropionyl glycine, protein kinase C antagonist chelerythrine, and mitochondrial KATP channel closer 5-hydroxydecanoate each blocked protection from 2 minutes of acidosis as they did for postconditioning.

活性氧簇清道夫N-2硫丙酰甘氨酸、蛋白激酶C拮抗剂白屈菜红碱、线粒体KATP通道关闭剂5-hydroxydecanoate都可以阻断两分钟的酸中毒和后条件的保护作用。

The nucleotide composition and the phylogenetic relationship among 14 species in Coreidae based on 412bp sequences of Cyt b gene were studied. The results showed: the average contents of T, C, A and G were 34.2 %、11.4 %、35.7 % and 18.7 %, respectively; The content of A+T (69.9 %) was obviously higher than that of G+C (30.1 %), and come up to 82.8% at the third position of codon.

以线粒体细胞色素b基因作为分子标记,首次对缘蝽科4亚科14种昆虫进行序列测定,获得Cytb基因412bp的序列片段,该片段中碱基T、C、A、G的平均含量分别为34.2%、11.4%、35.7 %和18.7 %,A+T平均含量为69.9 %,明显高于G+C含量(30.1 %)。

The interaction of Bcl-2 family proteins has regulatory effect to the apoptosis mediated by cytomicrosome.

Bcl-2家族蛋白成员间的相互作用对线粒体介导的细胞凋亡起调控作用。

This article gave an introduction to the discovery,construction,function of Bcl-2 family proteins and its roles in apoptosis induced by cytomicrosome.

现就Bcl-2家族蛋白的发现、结构、功能以及其对线粒体介导细胞调亡的作用机制做一下综述。

Cytomicrosome is the center of energy and metabolism of eukaryon.It is also a cellular organ with the critical adjusting function in processes of apoptosis signal.

线粒体是真核生物能量和代谢的中心,也是在细胞凋亡、信号转导中起关键调节作用的细胞器。

Methods Westernblot was employed to detect the intracellular expressions of caspase-9, hTERT, Bcl-2 and Bax, and mitochondrial and cytoplastic cytochrome C in HepG2 cells transfected by pSliencer 3.1-H1 neo-shTERT.

收集第20代hTERT基因RNAi真核表达载体论定转染细胞、对照细胞及未转染HepG2细饱,采用Western blot检测线粒体,胞质细胞色素C的表达及细胞内caspase-9、Bcl-2、Bax和hTERT的表达。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

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Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。