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In DM group, decreasing of density of axoplasm, deposition of fat droplets, swelling and vacuolization of mitochondria and separation or disintegration in lamellar of myelin sheath of the sciatic nerve fiber could be observed, which could be partially improved by EA and KBK.

电镜形态学分析见造模两个月的糖尿病大鼠坐骨神经髓鞘板层有分离现象,线粒体嵴紊乱,有的线粒体嵴完全消失形成空泡线粒体,轴浆内有大量脂肪颗粒沉积;经电针和渴痹康治疗后能减轻这些病理改变。4。无论是电针治疗还是渴痹康治疗均无明显降血糖作用。

In the present paper, we determined the mitochondrial DNA sequence 539bp of the high variable I D-loop regions(mtDNA HVS-I) for 243 domestic fowls 19 were Wugu (or black-bone chicken and 8 non-wugu breeds/races and 38 red jungle fowls (Gallus. Gallus. spadiceus and Gallus. Gallus. jabouillei). We also sequenced 289bp mitochondrial DNA of Cyt-b for 5 red jungle fowls; D-loop region (mtDNA HVS-I)for 19 individuals Guinea fowl and 18 individuals mule chickens (Guinea fowl $ x Wugu chickens^ ) respectively; mtDNA HVS- II of D-loop region for 34 individuals of domestic fowls and red jungle fowls in 690bp and the MC1R code gene in 945 bp.

本文共测定了19个乌骨鸡品种及8个非乌骨鸡品种的243个家鸡和38个原鸡个体(属于Gallus.gallus.spadiceus和Gallus.gallus.jabouillee亚种)的线粒体D-环高变区(mtDNA HVS-Ⅰ)539bp的部分序列,另外还测定了5个红原鸡个体线粒体细胞色素b的289 bp序列、19个珍珠鸡个体、18个骡鸡个体的线粒体D环高变区序列、30个家鸡个体线粒体D环非高变区的690bp序列以及34个家鸡及原鸡个体的MC1R基因编码区的945bp。

Results: The main ultrastructural changes after Cd treatment included swelling or vacuolation of mitochondria, increase of myelin figures in GCT cells, and swelling of mitochondria in the endothelial cells of peritubular capillaries. The changes above were most prominent at 7~15d, and swelling of the cytoplasm and slight karyolysis were also found. The morphology of GCT cells didn't recovery to normal yet after 30d.

结果:镉注射后24h,GCT细胞内出现不同程度的线粒体肿胀和空泡化,胞质和线粒体内可见髓样结构,GCT周围毛细血管内皮细胞线粒体肿胀。7~15天后上述改变明显加重,部分GCT细胞见胞质广泛肿胀和轻度核溶解,30天后细胞形态仍未恢复正常。

METHODS: Using Rhodamine123 (Rh123) as fluorescent prober to measure mitochondrial membrane potential and to record mitochondrial respiratory parameters with a Clark electrode ,the effect of diazoxide on mitochondrial membrane potential and mitochondrial respiration were investigated.

以罗丹明 12 3为荧光探针测定线粒体膜电位,氧电极法测线粒体呼吸,观察二氮嗪对正常大鼠心肌线粒体跨膜电位及呼吸的影响。

From using the same cervida for the Hydropotes intermis mitochondrial gene and its ratio of cytb,Detected 140 bp Variable sites,Insertion and deletion were not found.By comparison.

本文测定了麋鹿线粒体cytb基因全序列,并对麋鹿线粒体cytb基因全长1140bp进行了分析,利用同为偶蹄类鹿科的獐子线粒体cytb基因与其比对,检测到了140个变异位点,未发现插入和缺失。

To obtain more information of magnesium homeostasis in mitochondria, mTn-lacZ/LEU2 transposon library was transformed into mrs2 deletion mutant to screen for suppressor genes of MRS2. YMR166C, a member of mitochondrial carrier family, was identified as a suppressor gene of MRS2. Deletion of YMR166C gene can rescue the defects of mrs2 deletion mutant such as the decrease in mitochondrial magnesium concentration, Group II RNA splicing defect and growth defect on nonfermentable carbon source. For the first time we demonstrated YMR166C is involved in mitochondrial magnesium homeostasis.

为了增进对线粒体镁离子代谢调控基因的了解,利用酿酒酵母mTn-lacZ/LEU2转座子文库筛选MRS2的抑制基因,发现线粒体载体家族成员YMR166C基因的缺失可以挽救MRS2基因缺失的突变体的生长缺陷、II型内含子剪接缺陷,并可以调节线粒体镁离子浓度,首次发现YMR166C是线粒体镁代谢相关基因。

The result shows why mitochondrial suppression is so important to tumours: when they are unsuppressed, the tumour they are in stops growing.

为何线粒体抑制对肿瘤如此重要从试验结果可见一斑:当线粒体未受到抑制时,线粒体所在的肿瘤就会停止生长。

Ultrastructural examination revealed swelling and disorganization of cristae in myocardial mitochondria in traumatic group, and only slight swelling of mitochondria was found in intervention group.

电镜观察显示,创伤组心肌线粒体肿胀、嵴断裂,可见较大的线粒体,而干预组线粒体仅轻度肿胀。

Upon induction of apoptosis by diverse stimuli, Bax undergoes a conformational change and translocates to mitochondria, where it oligomerizes and forms pores that allow the release of cytochrome c and other cytotoxic foctors.

在被不同刺激诱导后,Bax的构象发生改变并转移到线粒体上。在线粒体膜上,它形成低聚物并使线粒体膜形成孔,使细胞色素c和其它细胞毒性因子可以排出。

Methods Mitochondria were isolated from rat cerebral cortex and purified with a homogenizing and centrifuging program.

组织匀浆梯度离心法分离并纯化大鼠脑皮质线粒体,氧电极法鉴定线粒体氧化呼吸及其偶联活性;体外无细胞线粒体3H UTP和3H Leucine掺入法分别测定细胞器的转录与翻译活性。

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