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Three methods, which were SDH activity, specific fluorescent probe(Rh123) and mitochondrial 16S rDNA gene amplification, were used to detect sperm mitochondria of Eriocheir sinensis, and three tissues——muscle, branchia and testis, were investigated as control.

以中华绒螫蟹肌肉、鳃和精巢为对照,从线粒体标志酶SDH活性、线粒体特异性荧光探针(Rh123)以及线粒体16S rDNA PCR扩增3个途径,探讨了该蟹精子线粒体的存在状况。

Methods A total of 144 healthy Wistar rats were randomly divided into three groups, ie, sham operation group, obstructive jaundice and biliary tract recanalization and obstructive jaundice and biliary tract recanalization plus rhALR to respectively observe, at selected time points, the changes of the liver and mitochondrial function as well as morphologic changes of the mitochondriae by electroscope.

144只健康Wistar大鼠随机分为SHAM组、BDO??RBF组、BDO??RBF??rhALR组,分别检测其线粒体功能及肝功能的变化情况,电镜观察各组大鼠肝细胞线粒体的形态学改变。结果胆道梗阻后,大鼠线粒体功能及肝功能均出现明显损伤(P<0.05或P<0.01),BDO??

The following five reviews published in this volume summarize recent discoveries in this area: DNA Replication and Transcription in Mammalian Mitochondria, Mitochondrial-Nuclear Communications, Translocation of Proteins into Mitochondria, The Machines that Divide and Fuse Mitochondria, and Why Do We Still Have a Maternally Inherited Mitochondrial DNA?

这一卷发表的以下五篇综述总结了这个领域的最新发现:哺乳动物线粒体中的DNA复制和转录,线粒体-细胞核通信,蛋白质到线粒体的迁移,分离和融合线粒体的机制及为什么我们仍然有一个母系遗传的线粒体DNA?

At the early stage of 〓 incubation, the mitochondrias were hyperplasia and swelling. After that the mitochondrias became vacuolation.

砷剂孵育早期即可见线粒体增生和肿胀,线粒体的改变早于细胞核染色质的变化。

Objective To clarify the relationship between oocyte maturation and mitochondria distribution by investigating the distribution of mitochondrial in immatured and matured human oocytes.

目的研究人未成熟和成熟卵母细胞中线粒体的分布,进一步阐明卵母细胞成熟和线粒体分布的关系。

The results indicate that idebenone improves mitochondrial oxidative metabolism in the brain and suggest a therapeutic potential of idebenone for MELAS.

本结果指明艾地苯醌改善了患者大脑线粒体的氧化代谢,同时並提示艾地苯醌具有治疗MELAS(线粒体脑肌病伴高乳酸血症和卒中样发作)的潜在效应。

Mitochondria have a small amount of their own DNA, which is called as mitochondrial DNA.

线粒体在自己的被叫做线粒体的DNA中数量很少。

The mtDNA defects and the role of mitochondrial lesion in the pathogenesis of RTT were investigated using mtDNA transfer by cybrid technique.

采用PCR,PCR-RFLP及DNA测序方法筛查MeCP2基因突变,采用融合细胞技术进行线粒体的转移实验探讨线粒体DNA缺陷以及线粒体损伤在RTT发病中的作用。

In addition, we studied the distribution of mitochondria in metaphase Ⅱ rabbit oocytes, zygotes and early embryos by two active mitochondria dyes and revealed the change of active mitochondria during early development of rabbit embryos. We also found that the distribution of microfilaments and microtubules have similar patterns to that of active mitochondria, which is an important basis for further development of animal cloning study using the recipient oocytes derived from rabbit.

此外,我们应用两种线粒体荧光染料研究了日本大耳白兔体内成熟卵母细胞、正常交配后收集的受精卵及早期体外发育胚胎的活性线粒体的位置变化,首次揭示了兔早期胚胎中活性线粒体的变化特征及其与微丝微管变化的相关性,为进一步开展以兔卵母细胞作为核移植受体的动物克隆研究打下了重要的基础。

There are abundant mitochondrion, they often locate near and enclosure chloroplast in alpine plants.

在所研究的4种高山植物中,线粒体的数量均较多,线粒体在细胞中的分布表现出不均一,且常分布在叶绿体附近,二者靠的很紧,常常可以观察到5~6个线粒体将叶绿体包围起来的现象。

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