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The heart,liver,spleen,lung,kidney,stomach and intestines of one agonal Cistoclemmys flavomarginatas were examined under transmission electron microscope.The results showed that a kind of short-stick bacteria were detected in heart,liver,spleen and kidney.

利用电子显微镜,对一只濒死黄缘盒龟的心、肝、脾、肺、肾、胃和肠道进行了检查,在心、肝、脾、肾均发现一种短杆状细菌感染,受染器官均出现明显的病理变化,如线粒体、高尔基体和内质网均肿大变形。

Results: The concentrations of sIL-2R, IL-6 and ET in serum in asthma group were higher than that in control group (P.01), Manifestation of the lung tissue under electron microscope: the lung organism of the animal was infiltrated lamellarly by eosinophile granulocyte in the model set.

结果:模型组血浆中sIL-2R、IL-6和ET的含量与正常对照组比较明显增高(P.01),肺组织光镜和电镜下观察显示:模型组动物的肺组织可见嗜酸性粒细胞片状浸润,Ⅱ型细胞部分线粒体脊突消失、膨胀,板层小体减少,有较多排空现象。

Identification of GC: Distinctive ultramicrostructure of GC can be found by transmission electron microscope: tubiform chondrosome, SER and adipoid is abundant, and the quantity of GCs reach 80%-85%.

透射电镜下可看到所收集细胞中颗粒细胞达80%~85%,其特征性的超微结构:胞质内出现管状嵴线粒体,滑面内质网丰富,出现电子密度较高的类脂滴。

We observed marophages pre-treated with ConA spread more rapidly on the well bottom compared with PBS injected controls under phase-contrast microscope. SEM of macrophages treated with ConA showed marked larger size and many surface ridges or ruffles and fine fingerlike processes or filopodia in comparison to controls, surface of which were smooth.

结果表明:①ConA处理后,倒置显微镜下可见Mφ粘附性增强;扫描电子显微镜下见其体积增大,指状突起增多;透射电子显微镜下见Mφ内线粒体等细胞器增多,伸出伪足包绕结核分枝杆菌并内吞,ConA活化的Mφ吞噬结核分枝杆菌数量增多,菌体的完整性受损。

In pituitary and thyroid of kidney Yang deficiency group, there were ultrastructural damages of rough endoplasmic reticulum dilation, mitochondria vacuolation, cells transformation and nucleus pycnosis.

正常组肾上腺束状带细胞排列成束,细胞内含有大量的线粒体和大而圆的脂滴,睾丸曲细精管生精上皮依次排列着不同生精细胞-精原细胞、初级精母细胞、精细胞、精子。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上,活细胞单层生长,胞浆较丰富,细胞核大小一致,有核仁×400 图2 SHEE培养细胞细胞核椭圆形,核仁较大,胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状,有伪足贴壁,表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁,呈星状或多角形,有丰富伪足和胞浆突,核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞,胞核和胞浆PI染色呈红色或淡红色,蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞,染色质凝集呈块状,右上角有一固缩细胞核TdT标记×400

These abnormal changes mainly occurred at two stages:(1) at meiotic prophase, almost half of Peiai 64S microsporocytes exhibited aberration, with sparse free ribosomes, underdeveloped mitochondria and many swollen endoplasmic reticula.

这些变化主要发生在两个阶段:(1)减数分裂前期,约半数的小孢子母细胞的胞质出现异常,游离核糖体稀少,并具有不发育线粒体和大量泡状内质网。

At the age of 19 weeks, we have observed ciliated-mucous cells, some cells presented with mucous granules in the supranuclear cytoplasm and a ciliating border with short growing shafts, while the other cells presented with large mucous granules as well as compound cilia.

小粘液颗粒细胞呈柱状,胞浆顶部含有少量球形的粘液颗粒,细胞表面有短小的微绒毛,细胞核大呈椭圆形,胞浆电子密度高,胞浆下部有粗面内质网和线粒体,核上部有高尔基体。

According to the observed of TEM,mitochondrial of the epithelial cells were vacuolized, the cristae and membrane had disappeared. Rough endoplasmic reticulums were expansion.

透射电镜观察上皮细胞线粒体空泡化、嵴和膜消失,粗面内质网扩张,杯状细胞内含有大量的粘原颗粒。

There are some little cells with diameter 4.5~8.3 μm in molecular layer. Purkinje cell is like a pear in Purkinje cell layer, it's base width is 17.8~25.6 μm, it's height is 18-30 μm. There are lots of rough endoplasmic reticulum, free ribosome, mitochondria, golgi body, lysosome, etc. in Purkinje cell. Granular cell ratio of nucleus to cytoplasm is high in granular layer.

分子层含少量小细胞,细胞大小在4.5~8.3μm; Purkinje细胞层细胞体呈梨状,底宽在17.8~25.6μm之间,高约为18~30μm,细胞中含有丰富的粗面内质网、游离核糖体、线粒体、高尔基体、溶酶体等;颗粒层的颗粒细胞细胞核大,细胞质少。

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