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线粒体状的

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The results showed as follow:①Chinese astringent persimmon were climacteric respiration fruit, the respiration climacteric stage occurred at speediness softening stage. Ethylene climacteric stage and respiration climacteric stage were almost occurred at one time.②The activities of EXP, PG, PE, CX all had increased, and EXP increased early with PG, PE, CX in turn in persimmon fruit during ripening and softening.③Concomitancy to fruit softening, ultrastructural changes in the mesocarp tissue were occurred at speediness softening stage, showed as disaggregation of middle layer of cell wall, appearance of fibre-like structures, disorganization of chloroplast and distortion of some mitochondria.

研究结果显示:①中国涩柿属呼吸跃变型果实,在其快速软化期出现呼吸跃变;乙烯跃变与呼吸跃变几乎同期出现;②在成熟软化过程中,EXP、PG、PE、CX的活性均有上升,EXP活性上升最早,随后依次是PG和PE、CX;PG活性上升幅度最大;③伴随果实软化,果肉组织超微结构在快速软化期中胶层解体、丝状体出现,叶绿体解体、部分线粒体变形;④1-MCP可延缓柿果实硬度下降,常温下延长保硬期5-7天。

The influences of INA were as follows: The mulcher of pollen parietes was disintegrated or disappeared in certain section and even the entire extine was deleted. The injuries of organelles in the pollen were demonstrated by plastosome peplos imperfect, vesicle sterility; concentric circles of the endoplasmic reticulum or fold arranged, to form the membranal inclusions by enwrapping part of the cytoplasm, and decrease of ribosome amount.

INA细菌对花粉的超微结构的影响表现为:使花粉壁的覆盖层在某些区域解体消失或整个花粉外壁缺失;使花粉中的细胞器受到破坏,包括线粒体被膜不完整,嵴呈囊泡状;内质网呈同心圆卷绕或折叠状排列包围部分细胞质而形成膜内含物;核糖体数量明显减少等。

Mitochondria was relatively little in size. Round primary lysosome with high electron-densed granules and secondary lysosome with high or low electron-densed granules were seen frequently. DCs contained many rough endoplasmic reticulum, the Golgi apparatus and ribosomes. The vacuoles with flocculent electron-densed granules were rare. Some special granules in cytoplasm were seen, whose surface like earphone were covered with a membrane. High electron-densed contents in the granules were near one side and the other side was bright. The nucleus became markedly small in volume, nephroid or hoofed in shape. The nucleus had little euchromatin and lots of heterochromatin under nuclear membrane.

子宫内膜癌组织DC超微结构特征如下:细胞形态不规则,与正常子宫内膜组织DC相比,胞膜较光滑,胞膜表面树突状胞浆突起显著减少,部分突起呈粗短状;胞质中线粒体相对少,圆形而电子密度高的初级溶酶体和不规则形且电子密度高低不一的次级溶酶体多见;高尔基体、粗面内质网、核糖体丰富;含微量絮状电子致密物的胞饮小泡显著减少;胞质中可见形态特殊的颗粒,该颗粒外周膜包裹,略呈圆形,中间部位稍弯曲,如耳机状,颗粒中由高电子致密物居于一侧,而另一侧则呈透亮状;胞核显著减小,居于胞质一侧,常呈肾形或马蹄形,核内常染色质较少,异染色质多边集于核膜下。

The influences of INA on ovule ultrastructure were also researched. The plasmolysis phenomenon occurred in the nucellar cells, a great deal of bubble structures existed in the cytoplasm, the sterility of plastosome was disarranged or the structure was entirely damaged and turned transparent.

INA细菌对胚珠的超微结构的影响表现为:使珠心细胞发生质壁分离现象,细胞质中存在大量泡状结构,线粒体的嵴紊乱或结构完全被破坏,呈透明状。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果:1、哇巴因作用于晶状体钠泵后,光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙,电镜下全层细胞空泡化、线粒体肿胀出现髓样结构,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后,光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离,电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后,光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整,电镜下细胞器丰富、细胞功能旺盛,RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后,光镜下晶状体质膜完整、细胞排列紧密规整,电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝,RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后,光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化,电镜下囊侧膜内有高电子密度带、细胞核仁丰富,RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后,光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏,电镜下质膜变性、线粒体肿胀,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

Results By using zinc-iodide-osmium staining, the tubulo-vesicular system of parietal cells of fundic gland was manifested as numerous fibrous structures under HVEM. The nematolysosomes was often demonstrated as branching in shape and anastomosed with each other making a network system in cytoplasm under the visual field of the three dimensional electron microscopy combined with AcPase cytochemistry.

结果高压电镜下显示,锌-碘-锇酸染色方法制备的标本胃底腺壁细胞的微管泡系统呈无数纤维状结构;AcPase结合三维电子显微镜观察显示,线性线粒体在胞质中常呈分支状并互相吻合成网。

The sex pheromone-producing gland of Ancylis.sativa Liu is amodified intersegmental membrane as a dorsal bag between eighth and ninthabdominal segments.

对枣粘虫雌蛾性信息素腺体的扫描和透射电镜观察表明,枣粘虫性信息素腺体是由第八、九腹节间的节间膜特化而成,是位于背部的一个囊状结构,它由两部分构成,前部的方形囊状体和后部的三角形囊状体,二者之间有一突起的脊;雌蛾静止时,腺体随第八和第九腹节一起嵌缩于第七腹节内,求偶时,腹部末端外伸,腺体细胞表皮外露,释放性信息素;腺体细胞呈方形,其表面覆盖几丁质表皮,表皮顶端无孔;细胞核很大,呈椭圆形,由双层核膜包被,位于细胞中下部,内有丰富的染色质;细胞质内有丰富的内质网,其内还有大量的大小不等的脂肪滴,以及线粒体和溶酶体等细胞器。

The results showed that with the extension of AVM treatment time, the relative neuron survival rate and the mitochondria fumaric reductase activity decreased, the percentage of neuronal apoptosis increased, the mitochondria swelled, the mitochondrial structure was damaged, cristae were broken and dissolved and there were vacuoli in base.

结果显示,随着染毒时间的延长,细胞的相对存活率下降;线粒体琥珀酸脱氢酶活力降低;PI-/Rh123-细胞百分数增加;线粒体肿胀,结构不清晰,嵴断裂、溶解、基质中有片状空腔现象。

By electron microscope we can find the Shenqi group's cancer nuclear chromatin is partly condensing and a few with screen opening changing, the membrane is invaginating , the mitochondrias are mostly swelling and the number is decreased, with the cristae'number decreased or breakon even diminished and destructured in different degree, parts of the mitochondrias are bubbling changing.

细胞膜内陷,线粒体数量减少,多数肿胀,嵴减少或断裂,甚至消失,有不同程度的变性,部分线粒体呈空泡状。

Before treatment,there are abundant microvtlli on the cell surface,many filoplodia on the cell rim,few of endoplasmic reticulums , Golgi complexes and mitochondrions with abnormal structure and lots of free ribosomes in the cytoplasm. The shape of nucleus is irregular with high nuclear-cytoplasmic ratio, many dark pellets of heteromatin and a few of nucleoluses in which there ar...

经1.5×10~2g/L地塞米松处理后,细胞表面微绒毛和丝状伪足显著减少或消失,出现皱褶状和小泡状结构;细胞核形规则,核质比值减小,异染色质团块减少,核仁数目减少、结构致密;细胞质中粗糙型内质网、高尔基体、线粒体数量增多,形态结构典型,游离核糖体减少,这些变化说明地塞米松改变了MGc80-3细胞的超微结构特征,具有明显的诱导分化作用。

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